Restriction map for
pFN21AE6114
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BamHI BanII BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaWI
BseAI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI Eam1105I EarI Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HinfI HpaI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MfeI MluI MroI MseI MslI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 298 GGCGCC G[GA]^CG[CT]C
AlwI 78 GGATC GGATC(4/5)
Aor51HI 185 AGCGCT AGC^GCT
AspI 84 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 100 CCCGAG C^[CT]CG[GA]G
AvaII 384 GGACC G^G[AT]CC
BalI 372 TGGCCA TGG^CCA
BbeI 298 GGCGCC GGCGC^C
BglI 58 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 15 GCTGAGC GC^T[ACGT]AGC
BmyI 449 GTGCCC G[AGT]GC[ACT]^C
BpmI 355 CTGGAG CTGGAG(16/14)
Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC
BsaBI 210 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 298 GGCGCC G[GA]^CG[CT]C
BsaI 145 GAGACC GGTCTC(1/5)
BsgI 181 GTGCAG GTGCAG(16/14)
BsiEI 222 CGGCCG CG[GA][CT]^CG
BsoBI 100 CCCGAG C^[CT]CG[GA]G
Bsp1286I 449 GTGCCC G[AGT]GC[ACT]^C
BsrBI 471 CCGCTC CCGCTC(-3/-3)
BsrI 375 CCAGT ACTGG(1/-1)
BstYI 78 GGATCT [GA]^GATC[CT]
CelII 15 GCTGAGC GC^T[ACGT]AGC
DsaI 225 CCGTGG C^C[GA][CT]GG
EagI 222 CGGCCG C^GGCCG
EclXI 222 CGGCCG C^GGCCG
Eco47III 185 AGCGCT AGC^GCT
Eco52I 222 CGGCCG C^GGCCG
EcoNI 117 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 305 CCAAGG C^C[AT][AT]GG
Hin1I 298 GGCGCC G[GA]^CG[CT]C
HphI 281 TCACC GGTGA(8/7)
KasI 298 GGCGCC G^GCGCC
MamI 210 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 278 TCTTC GAAGA(8/7)
MflI 78 GGATCT [GA]^GATC[CT]
MluNI 372 TGGCCA TGG^CCA
MscI 372 TGGCCA TGG^CCA
NarI 298 GGCGCC GG^CGCC
PflFI 84 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 232 CTGCAG CTGCA^G
SfcI 232 CTGCAG C^T[GA][CT]AG
SfoI 298 GGCGCC GGC^GCC
StuI 56 AGGCCT AGG^CCT
StyI 305 CCAAGG C^C[AT][AT]GG
Tth111I 84 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 78 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 240 GTAC GT^AC
262 GTAC
AlwNI 172 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
340 CAGGAGCTG
BcnI 51 CCGGG CC^[GC]GG
445 CCCGG
BsmFI 468 GTCCC GGGAC(10/14)
480 GTCCC
Cfr13I 98 GGCCC G^G[ACGT]CC
384 GGACC
DpnII 79 GATC ^GATC
405 GATC
EaeI 222 CGGCCG [CT]^GGCC[GA]
372 TGGCCA
FauI 470 CCCGC CCCGC(4/6)
258 GCGGG
HapII 51 CCGG C^CGG
446 CCGG
HpaII 51 CCGG C^CGG
446 CCGG
MboI 79 GATC ^GATC
405 GATC
MspI 51 CCGG C^CGG
446 CCGG
NciI 51 CCGGG CC^[GC]GG
445 CCCGG
NdeII 79 GATC ^GATC
405 GATC
PvuII 244 CAGCTG CAG^CTG
415 CAGCTG
RsaI 240 GTAC GT^AC
262 GTAC
Sau3AI 79 GATC ^GATC
405 GATC
Sau96I 98 GGCCC G^G[ACGT]CC
384 GGACC
SfaNI 204 GATGC GCATC(5/9)
215 GCATC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BanI BbvI BsaJI
BseRI BsiYI BslI BsmAI BssKI
BstF5I BstNI Cac8I CfoI DdeI
EcoRII Fnu4HI FokI HaeII HaeIII
HhaI HinP1I ItaI MnlI MspA1I
MvaI MwoI NlaIII NlaIV PflMI
ScrFI TseI TspRI Van91I