Restriction map for
pFN21AE1493
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvrII BalI BamHI BbeI
BbrPI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI HaeII HapII
HgaI Hin1I HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tru9I Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwNI 677 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 746 GAGCTC G[AT]GC[AT]^C
AviII 407 TGCGCA TGC^GCA
BanI 271 GGCACC G^G[CT][GA]CC
BbsI 280 GAAGAC GAAGAC(2/6)
BbvCI 251 CCTCAGC CCTCAGC(-5/-2)
BciVI 223 GTATCC GTATCC(6/5)
BclI 800 TGATCA T^GATCA
BfaI 337 CTAG C^TAG
BglI 667 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 280 GAAGAC GAAGAC(2/6)
BsaAI 439 TACGTG [CT]AC^GT[GA]
BsiHKAI 746 GAGCTC G[AT]GC[AT]^C
BsmAI 502 GTCTC GTCTC(1/5)
BsmBI 501 CGTCTC CGTCTC(1/5)
BspHI 387 TCATGA T^CATGA
BstF5I 570 GGATG GGATG(2/0)
BstYI 821 AGATCC [GA]^GATC[CT]
DraIII 400 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 189 GACAGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 275 CCATGG C^C[GA][CT]GG
EcoRI 76 GAATTC G^AATTC
FbaI 800 TGATCA T^GATCA
FokI 570 GGATG GGATG(9/13)
FspI 407 TGCGCA TGC^GCA
HincII 424 GTCAAC GT[CT]^[GA]AC
HindII 424 GTCAAC GT[CT]^[GA]AC
MaeI 337 CTAG C^TAG
MaeII 440 ACGT A^CGT
MflI 821 AGATCC [GA]^GATC[CT]
NcoI 275 CCATGG C^CATGG
NdeI 694 CATATG CA^TATG
NspI 341 ACATGC [GA]CATG^[CT]
RcaI 387 TCATGA T^CATGA
SacI 746 GAGCTC GAGCT^C
SapI 39 GCTCTTC GCTCTTC(1/4)
SfaNI 90 GATGC GCATC(5/9)
SspI 599 AATATT AAT^ATT
XhoII 821 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 76 GAATTC [GA]^AATT[CT]
829 GAATTT
AlwI 822 GATCC GGATC(4/5)
134 GGATC
ApoI 76 GAATTC [GA]^AATT[CT]
829 GAATTT
AvaII 444 GGTCC G^G[AT]CC
788 GGACC
BcgI 128 GCAAAGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
491 GCATTGTTGTCG
BpmI 235 CTGGAG CTGGAG(16/14)
727 CTGGAG
BsrDI 264 CATTGC GCAATG(2/0)
482 GCAATG
CfoI 408 GCGC GCG^C
753 GCGC
DdeI 252 CTCAG C^T[ACGT]AG
433 CTCAG
EcoT14I 96 CCAAGG C^C[AT][AT]GG
275 CCATGG
HhaI 408 GCGC GCG^C
753 GCGC
HinP1I 408 GCGC G^CGC
753 GCGC
HinfI 378 GAATC G^A[ACGT]TC
863 GATTC
PflMI 177 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
228 CCACTGCCTGG
SfcI 211 CTACAG C^T[GA][CT]AG
452 CTGTAG
StyI 96 CCAAGG C^C[AT][AT]GG
275 CCATGG
TfiI 378 GAATC G^A[AT]TC
863 GATTC
Tsp45I 115 GTCAC ^GT[GC]AC
187 GTGAC
TspRI 229 CACTG CA[GC]TG[ACGT][ACGT]^
605 CAGTG
Van91I 177 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
228 CCACTGCCTGG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI BanII BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I Cfr13I DpnII EarI
EcoRII Fnu4HI HaeIII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI Tsp509I