Restriction map for
pFN21AE3667
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 105 ACATGT A^C[GA][CT]GT
BanI 302 GGCACC G^G[CT][GA]CC
BanII 156 GGGCTC G[GA]GC[CT]^C
BbsI 213 GAAGAC GAAGAC(2/6)
BbvI 261 GCTGC GCAGC(8/12)
BglII 164 AGATCT A^GATCT
BmyI 156 GGGCTC G[AGT]GC[ACT]^C
BpmI 310 CTCCAG CTGGAG(16/14)
BpuAI 213 GAAGAC GAAGAC(2/6)
BsaI 382 GAGACC GGTCTC(1/5)
BsaWI 110 TCCGGT [AT]^CCGG[AT]
BsgI 133 GTGCAG GTGCAG(16/14)
Bsp1286I 156 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 105 ACATGT A^CATGT
BstF5I 219 CATCC GGATG(2/0)
BstXI 326 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 164 AGATCT [GA]^GATC[CT]
DsaI 175 CCACGG C^C[GA][CT]GG
EarI 466 CTCTTC CTCTTC(1/4)
EcoNI 306 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 122 CCAAGG C^C[AT][AT]GG
Fnu4HI 261 GCTGC GC^[ACGT]GC
FokI 219 CATCC GGATG(9/13)
HapII 111 CCGG C^CGG
HinfI 280 GAGTC G^A[ACGT]TC
HpaII 111 CCGG C^CGG
ItaI 261 GCTGC GC^[ACGT]GC
Ksp632I 466 CTCTTC CTCTTC(1/4)
MaeIII 394 GTGAC ^GT[ACGT]AC
MflI 164 AGATCT [GA]^GATC[CT]
MspI 111 CCGG C^CGG
MwoI 4 GCTCCCAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 77 CATATG CA^TATG
PleI 280 GAGTC GAGTC(4/5)
SexAI 140 ACCAGGT A^CC[AT]GGT
StyI 122 CCAAGG C^C[AT][AT]GG
TaqI 356 TCGA T^CGA
TseI 261 GCTGC G^C[AT]GC
Tsp45I 394 GTGAC ^GT[GC]AC
TthHB8I 356 TCGA T^CGA
XhoII 164 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AluI 260 AGCT AG^CT
328 AGCT
AvaII 138 GGACC G^G[AT]CC
323 GGTCC
BseRI 307 CTCCTC GAGGAG(10/8)
256 GAGGAG
BsrDI 349 GCAATG GCAATG(2/0)
401 GCAATG
BsrI 414 ACTGG ACTGG(1/-1)
248 CCAGT
Cfr13I 138 GGACC G^G[ACGT]CC
323 GGTCC
DpnII 165 GATC ^GATC
354 GATC
HphI 240 GGTGA GGTGA(8/7)
286 GGTGA
MboI 165 GATC ^GATC
354 GATC
MseI 55 TTAA T^TAA
169 TTAA
NdeII 165 GATC ^GATC
354 GATC
Sau3AI 165 GATC ^GATC
354 GATC
Sau96I 138 GGACC G^G[ACGT]CC
323 GGTCC
SfaNI 67 GATGC GCATC(5/9)
446 GCATC
Tru9I 55 TTAA T^TAA
169 TTAA
TspRI 249 CAGTG CA[GC]TG[ACGT][ACGT]^
332 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BsaJI BsiYI BslI BsmAI BssKI
BstNI DdeI EcoRII MboII MnlI
MvaI NlaIII NlaIV NspI ScrFI