Restriction map for
pFN21AE3588
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AccIII AclI AflII AflIII AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KasI MamI MfeI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 2086 GACGTC GACGT^C
Acc65I 1019 GGTACC G^GTACC
AccI 1921 GTCTAC GT^[AC][GT]AC
AcsI 294 GAATTT [GA]^AATT[CT]
AgeI 1016 ACCGGT A^CCGGT
Aor51HI 1559 AGCGCT AGC^GCT
ApaI 2156 GGGCCC GGGCC^C
ApoI 294 GAATTT [GA]^AATT[CT]
Asp718I 1019 GGTACC G^GTACC
AvaI 1695 CTCGGG C^[CT]CG[GA]G
BalI 1376 TGGCCA TGG^CCA
BcgI 1805 GCAGCTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1258 CTAG C^TAG
BglI 1348 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsoBI 1695 CTCGGG C^[CT]CG[GA]G
Bsp1407I 171 TGTACA T^GTACA
BspMI 1022 ACCTGC ACCTGC(4/8)
BsrDI 1942 CATTGC GCAATG(2/0)
BsrFI 1016 ACCGGT [GA]^CCGG[CT]
BsrGI 171 TGTACA T^GTACA
BssSI 505 CACGAG CACGAG(-5/-1)
BstAPI 1067 GCATCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 298 TTCGAA TT^CGAA
Cfr10I 1016 ACCGGT [GA]^CCGG[CT]
DrdI 693 GACAGATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1559 AGCGCT AGC^GCT
EcoNI 624 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2310 ATGCAT ATGCA^T
KpnI 1019 GGTACC GGTAC^C
KspI 1042 CCGCGG CCGC^GG
MaeI 1258 CTAG C^TAG
MluNI 1376 TGGCCA TGG^CCA
MscI 1376 TGGCCA TGG^CCA
NsiI 2310 ATGCAT ATGCA^T
NspI 220 GCATGT [GA]CATG^[CT]
NspV 298 TTCGAA TT^CGAA
PinAI 1016 ACCGGT A^CCGGT
PshAI 1254 GACGCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 532 CTGCAG CTGCA^G
SacI 1306 GAGCTC GAGCT^C
SacII 1042 CCGCGG CCGC^GG
SapI 450 GCTCTTC GCTCTTC(1/4)
SfuI 298 TTCGAA TT^CGAA
SspBI 171 TGTACA T^GTACA
Tsp509I 295 AATT ^AATT
XcmI 2129 CCACAAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 367 CGCG CG^CG
1043 CGCG
AcyI 1498 GACGCC G[GA]^CG[CT]C
2086 GACGTC
AocI 513 CCTCAGG CC^T[ACGT]AGG
2096 CCTCAGG
Asp700I 969 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1482 GAAGTGCTTC
AspHI 115 GTGCTC G[AT]GC[AT]^C
1306 GAGCTC
BanII 1306 GAGCTC G[GA]GC[CT]^C
2156 GGGCCC
BbvCI 1656 CCTCAGC CCTCAGC(-5/-2)
1683 CCTCAGC
BlpI 412 GCTGAGC GC^T[ACGT]AGC
1590 GCTGAGC
Bpu1102I 412 GCTGAGC GC^T[ACGT]AGC
1590 GCTGAGC
BsaHI 1498 GACGCC G[GA]^CG[CT]C
2086 GACGTC
BsaWI 1016 ACCGGT [AT]^CCGG[AT]
1432 ACCGGA
BsiHKAI 115 GTGCTC G[AT]GC[AT]^C
1306 GAGCTC
BstUI 367 CGCG CG^CG
1043 CGCG
BstXI 1223 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2048 CCAAACCCCTGG
Bsu36I 513 CCTCAGG CC^T[ACGT]AGG
2096 CCTCAGG
CelII 412 GCTGAGC GC^T[ACGT]AGC
1590 GCTGAGC
EarI 451 CTCTTC CTCTTC(1/4)
1582 GAAGAG
Eco81I 513 CCTCAGG CC^T[ACGT]AGG
2096 CCTCAGG
EcoT14I 1199 CCTTGG C^C[AT][AT]GG
1505 CCAAGG
HaeII 1559 AGCGCT [GA]GCGC^[CT]
2013 GGCGCT
Hin1I 1498 GACGCC G[GA]^CG[CT]C
2086 GACGTC
Ksp632I 451 CTCTTC CTCTTC(1/4)
1582 GAAGAG
MaeII 1883 ACGT A^CGT
2087 ACGT
MslI 205 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1224 CACTGTGGTG
MvnI 367 CGCG CG^CG
1043 CGCG
PflMI 209 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1056 CCAGCTGCTGG
SexAI 191 ACCTGGT A^CC[AT]GGT
1166 ACCTGGT
SmlI 96 CTTGAG C^T[CT][GA]AG
1467 CTTGAG
StuI 342 AGGCCT AGG^CCT
389 AGGCCT
StyI 1199 CCTTGG C^C[AT][AT]GG
1505 CCAAGG
Van91I 209 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1056 CCAGCTGCTGG
XmnI 969 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1482 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII