Restriction map for
pFN21AE4521
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BfaI BfrI BglI BlnI
BpuAI BsaBI BsaI BseRI BsgI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BssHII
BssSI Bst1107I BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
FseI FspI HincII HindII HindIII
HpaI KasI KspI MaeI MamI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 364 GACGTC GACGT^C
AflIII 1340 ACGTGT A^C[GA][CT]GT
AlwI 996 GGATC GGATC(4/5)
AlwNI 177 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 43 GGGCCC GGGCC^C
Asp700I 1122 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 139 GAGCAC G[AT]GC[AT]^C
AvaI 942 CCCGAG C^[CT]CG[GA]G
AvaII 309 GGACC G^G[AT]CC
BalI 1208 TGGCCA TGG^CCA
BciVI 1359 GTATCC GTATCC(6/5)
BclI 884 TGATCA T^GATCA
BlpI 72 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 72 GCTCAGC GC^T[ACGT]AGC
BsaAI 1339 TACGTG [CT]AC^GT[GA]
BsiHKAI 139 GAGCAC G[AT]GC[AT]^C
BsoBI 942 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1343 TGTACA T^GTACA
BsrGI 1343 TGTACA T^GTACA
BstAPI 1182 GCAATATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1004 TTCGAA TT^CGAA
CelII 72 GCTCAGC GC^T[ACGT]AGC
EagI 219 CGGCCG C^GGCCG
EclXI 219 CGGCCG C^GGCCG
Eco52I 219 CGGCCG C^GGCCG
EcoT22I 716 ATGCAT ATGCA^T
FbaI 884 TGATCA T^GATCA
HgaI 160 GACGC GACGC(5/10)
MluNI 1208 TGGCCA TGG^CCA
MscI 1208 TGGCCA TGG^CCA
NdeI 1157 CATATG CA^TATG
NsiI 716 ATGCAT ATGCA^T
NspI 533 GCATGC [GA]CATG^[CT]
NspV 1004 TTCGAA TT^CGAA
PstI 556 CTGCAG CTGCA^G
SexAI 398 ACCTGGT A^CC[AT]GGT
SfuI 1004 TTCGAA TT^CGAA
SphI 533 GCATGC GCATG^C
SspBI 1343 TGTACA T^GTACA
XmnI 1122 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1257 GGTACC G^GTACC
1540 GGTACC
AccI 367 GTCTAC GT^[AC][GT]AC
1336 GTCTAC
AccIII 339 TCCGGA T^CCGGA
1019 TCCGGA
AcsI 573 GAATTT [GA]^AATT[CT]
659 AAATTC
AcyI 160 GACGCC G[GA]^CG[CT]C
364 GACGTC
ApoI 573 GAATTT [GA]^AATT[CT]
659 AAATTC
Asp718I 1257 GGTACC G^GTACC
1540 GGTACC
BanII 43 GGGCCC G[GA]GC[CT]^C
465 GGGCTC
BglII 560 AGATCT A^GATCT
620 AGATCT
BpmI 289 CTGGAG CTGGAG(16/14)
322 CTGGAG
BsaHI 160 GACGCC G[GA]^CG[CT]C
364 GACGTC
BsaWI 339 TCCGGA [AT]^CCGG[AT]
1019 TCCGGA
BseAI 339 TCCGGA T^CCGGA
1019 TCCGGA
BsiEI 219 CGGCCG CG[GA][CT]^CG
1458 CGACCG
BsmAI 761 GAGAC GTCTC(1/5)
1428 GTCTC
BspEI 339 TCCGGA T^CCGGA
1019 TCCGGA
BsrFI 103 ACCGGC [GA]^CCGG[CT]
251 ACCGGC
BsrI 416 ACTGG ACTGG(1/-1)
764 ACTGG
BstEII 18 GGTGACC G^GT[ACGT]ACC
1026 GGTCACC
BstPI 18 GGTGACC G^GT[ACGT]ACC
1026 GGTCACC
BstXI 626 CCAACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1058 CCATATCAGTGG
Cfr10I 103 ACCGGC [GA]^CCGG[CT]
251 ACCGGC
EcoO65I 18 GGTGACC G^GT[ACGT]ACC
1026 GGTCACC
Hin1I 160 GACGCC G[GA]^CG[CT]C
364 GACGTC
KpnI 1257 GGTACC GGTAC^C
1540 GGTACC
MroI 339 TCCGGA T^CCGGA
1019 TCCGGA
MseI 1624 TTAA T^TAA
1655 TTAA
SfcI 556 CTGCAG C^T[GA][CT]AG
1283 CTACAG
SspI 684 AATATT AAT^ATT
1184 AATATT
TaqI 568 TCGA T^CGA
1005 TCGA
Tru9I 1624 TTAA T^TAA
1655 TTAA
Tsp509I 574 AATT ^AATT
660 AATT
TthHB8I 568 TCGA T^CGA
1005 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI BanI
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
TspRI XhoII