##################################
# NO CUT ENZYMES (  217 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DsaI        EaeI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       ItaI        
KasI        KpnI        KspI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        TseI        Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AcsI          309  AAATTT               [GA]^AATT[CT]
AfaI           57  GTAC                 GT^AC
AhdI           33  GACCAACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          309  AAATTT               [GA]^AATT[CT]
AspEI          33  GACCAACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BspMI          93  GCAGGT               ACCTGC(4/8)
DdeI          163  CTCAG                C^T[ACGT]AG
DrdI          142  GACAAAGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I       33  GACCAACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          205  GAAGAG               CTCTTC(1/4)
HinfI         159  GACTC                G^A[ACGT]TC
HphI          361  GGTGA                GGTGA(8/7)
Ksp632I       205  GAAGAG               CTCTTC(1/4)
MaeIII        106  GTGAC                ^GT[ACGT]AC
MboII         205  GAAGA                GAAGA(8/7)
MnlI            7  GAGG                 CCTC(7/6)
MseI          326  TTAA                 T^TAA
MspA1I        196  CAGCTG               C[AC]G^C[GT]G
MwoI           10  GCTTTCTGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII         60  CATG                 CATG^
PleI          159  GACTC                GAGTC(4/5)
PstI          224  CTGCAG               CTGCA^G
PvuII         196  CAGCTG               CAG^CTG
RsaI           57  GTAC                 GT^AC
SfcI          224  CTGCAG               C^T[GA][CT]AG
Tru9I         326  TTAA                 T^TAA
Tsp45I        106  GTGAC                ^GT[GC]AC
TspRI         222  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI           86  CCACTAGGCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AluI           29  AGCT                 AG^CT
              197  AGCT                
BfaI           70  CTAG                 C^TAG
               89  CTAG                
BsmAI         140  GAGAC                GTCTC(1/5)
              266  GAGAC               
BssKI          23  CCTGG                ^CC[ACGT]GG
              258  CCTGG               
BstNI          23  CCTGG                CC^[AT]GG
              258  CCTGG               
EcoRII         23  CCTGG                ^CC[AT]GG
              258  CCTGG               
MaeI           70  CTAG                 C^TAG
               89  CTAG                
MvaI           23  CCTGG                CC^[AT]GG
              258  CCTGG               
ScrFI          23  CCTGG                CC^[ACGT]GG
              258  CCTGG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

BstF5I      FokI        Tsp509I