##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BseAI       BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MfeI        MluI        MroI        MseI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AciI          386  CCGC                 CCGC(-3/-1)
Alw44I        348  GTGCAC               G^TGCAC
ApaI          620  GGGCCC               GGGCC^C
ApaLI         348  GTGCAC               G^TGCAC
AspHI         348  GTGCAC               G[AT]GC[AT]^C
AvaII          38  GGACC                G^G[AT]CC
BalI          532  TGGCCA               TGG^CCA
BbsI          269  GTCTTC               GAAGAC(2/6)
BbvCI         418  CCTCAGC              CCTCAGC(-5/-2)
BpuAI         269  GTCTTC               GAAGAC(2/6)
BsaI          204  GGTCTC               GGTCTC(1/5)
BsaWI         759  ACCGGA               [AT]^CCGG[AT]
BsiEI         391  CGGTCG               CG[GA][CT]^CG
BsiHKAI       348  GTGCAC               G[AT]GC[AT]^C
BsmBI         395  CGTCTC               CGTCTC(1/5)
BsrBI         386  CCGCTC               CCGCTC(-3/-3)
BsrDI         320  GCAATG               GCAATG(2/0)
BssSI         145  CTCGTG               CACGAG(-5/-1)
BstXI          10  CCACATCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         544  AGATCC               [GA]^GATC[CT]
DraIII        351  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          384  GACCGCTCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          365  CTCTTC               CTCTTC(1/4)
EcoNI         144  CCTCGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HphI          608  TCACC                GGTGA(8/7)
Ksp632I       365  CTCTTC               CTCTTC(1/4)
MflI          544  AGATCC               [GA]^GATC[CT]
MluNI         532  TGGCCA               TGG^CCA
MscI          532  TGGCCA               TGG^CCA
MslI          573  CATCCTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII        477  CATG                 CATG^
NspI          476  ACATGC               [GA]CATG^[CT]
PpuMI          37  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          683  CTGCAG               CTGCA^G
SfcI          683  CTGCAG               C^T[GA][CT]AG
Tsp45I        158  GTGAC                ^GT[GC]AC
XcmI           10  CCACATCCCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         544  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AlwNI         400  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              481  CAGACTCTG           
BpmI           62  CTCCAG               CTGGAG(16/14)
              454  CTGGAG              
BseRI         715  GAGGAG               GAGGAG(10/8)
              718  GAGGAG              
BsmAI         205  GTCTC                GTCTC(1/5)
              396  GTCTC               
BsmFI          37  GGGAC                GGGAC(10/14)
              338  GGGAC               
BsrI           95  ACTGG                ACTGG(1/-1)
              530  ACTGG               
HinfI         483  GACTC                G^A[ACGT]TC
              516  GACTC               
PleI          483  GACTC                GAGTC(4/5)
              516  GACTC               
SfaNI         450  GATGC                GCATC(5/9)
              572  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AluI        AlwI        BanII       BbvI        BcnI        
BmyI        BsaJI       BsgI        BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HpaII       ItaI        MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIV       PvuII       
Sau3AI      Sau96I      ScrFI       TseI        TspRI