Restriction map for
pFN21AE6428
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI Asp700I Asp718I AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BlnI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CpoI
DraI DraIII DrdI DsaI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HindIII KasI KpnI KspI MaeII
MamI MluI MroI NarI NcoI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Van91I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
AccI 574 GTCGAC GT^[AC][GT]AC
AcsI 286 AAATTC [GA]^AATT[CT]
AcyI 184 GGCGTC G[GA]^CG[CT]C
AhdI 663 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 596 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 880 GGGCCC GGGCC^C
ApoI 286 AAATTC [GA]^AATT[CT]
AseI 415 ATTAAT AT^TAAT
AsnI 415 ATTAAT AT^TAAT
AspEI 663 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1080 GAGCAC G[AT]GC[AT]^C
AspI 365 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 6 CCCGGG C^[CT]CG[GA]G
AvaII 709 GGTCC G^G[AT]CC
BalI 198 TGGCCA TGG^CCA
BcgI 1160 GCAACAATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 236 AGATCT A^GATCT
BlpI 99 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 99 GCTGAGC GC^T[ACGT]AGC
BsaHI 184 GGCGTC G[GA]^CG[CT]C
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsiHKAI 1080 GAGCAC G[AT]GC[AT]^C
BsoBI 6 CCCGGG C^[CT]CG[GA]G
BspDI 949 ATCGAT AT^CGAT
BspHI 1298 TCATGA T^CATGA
BspMI 744 ACCTGC ACCTGC(4/8)
BsrFI 46 GCCGGC [GA]^CCGG[CT]
BssSI 1039 CACGAG CACGAG(-5/-1)
BstYI 236 AGATCT [GA]^GATC[CT]
CelII 99 GCTGAGC GC^T[ACGT]AGC
Cfr10I 46 GCCGGC [GA]^CCGG[CT]
ClaI 949 ATCGAT AT^CGAT
DraII 879 GGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 41 CGGCCG C^GGCCG
Eam1105I 663 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 232 GAAGAG CTCTTC(1/4)
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
EcoNI 1020 CCTATGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 879 GGGGCCC [GA]G^G[ACGT]CC[CT]
FauI 176 CCCGC CCCGC(4/6)
HgaI 185 GCGTC GACGC(5/10)
Hin1I 184 GGCGTC G[GA]^CG[CT]C
HpaI 1320 GTTAAC GTT^AAC
Ksp632I 232 GAAGAG CTCTTC(1/4)
MfeI 319 CAATTG C^AATTG
MflI 236 AGATCT [GA]^GATC[CT]
MluNI 198 TGGCCA TGG^CCA
MscI 198 TGGCCA TGG^CCA
MspA1I 202 CAGCTG C[AC]G^C[GT]G
MunI 319 CAATTG C^AATTG
NaeI 46 GCCGGC GCC^GGC
NdeI 665 CATATG CA^TATG
NgoMIV 46 GCCGGC G^CCGGC
NotI 40 GCGGCCGC GC^GGCCGC
PflFI 365 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 415 ATTAAT AT^TAAT
PvuII 202 CAGCTG CAG^CTG
RcaI 1298 TCATGA T^CATGA
SalI 574 GTCGAC G^TCGAC
ScaI 1003 AGTACT AGT^ACT
SfcI 835 CTACAG C^T[GA][CT]AG
SmaI 6 CCCGGG CCC^GGG
SmlI 713 CTCAAG C^T[CT][GA]AG
SspI 1177 AATATT AAT^ATT
Tth111I 365 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 236 AGATCT [GA]^GATC[CT]
XmaCI 6 CCCGGG C^CCGGG
XmaI 6 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 866 GTAC GT^AC
1004 GTAC
BanII 25 GGGCTC G[GA]GC[CT]^C
880 GGGCCC
BbvI 138 GCAGC GCAGC(8/12)
204 GCTGC
BfaI 79 CTAG C^TAG
1138 CTAG
BsgI 205 CTGCAC GTGCAG(16/14)
975 CTGCAC
BsmAI 1049 GAGAC GTCTC(1/5)
984 GTCTC
BsmI 641 GAATGC GAATGC(1/-1)
1308 GAATGC
BstAPI 739 GCATTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
772 GCAAATCCTGC
HincII 574 GTCGAC GT[CT]^[GA]AC
1320 GTTAAC
HindII 574 GTCGAC GT[CT]^[GA]AC
1320 GTTAAC
HphI 330 TCACC GGTGA(8/7)
1289 TCACC
MaeI 79 CTAG C^TAG
1138 CTAG
MslI 331 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1149 CATTTTGGTG
NheI 78 GCTAGC G^CTAGC
1137 GCTAGC
RsaI 866 GTAC GT^AC
1004 GTAC
SfaNI 931 GATGC GCATC(5/9)
424 GCATC
TseI 138 GCAGC G^C[AT]GC
204 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI AlwI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboI MboII MnlI MseI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I