Restriction map for
pFN21AE1374
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# NO CUT ENZYMES ( 131 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BamHI BbrPI BbvCI BciVI
BclI BfaI BfrI BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp509I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 1131 GACGTC GACGT^C
AfaI 120 GTAC GT^AC
Alw44I 1075 GTGCAC G^TGCAC
AlwI 1085 GGATC GGATC(4/5)
ApaLI 1075 GTGCAC G^TGCAC
AspI 391 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 230 TGGCCA TGG^CCA
BbeI 300 GGCGCC GGCGC^C
BcgI 156 CGAGGAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 881 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1221 AGATCT A^GATCT
BlpI 279 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 279 GCTGAGC GC^T[ACGT]AGC
BsgI 490 GTGCAG GTGCAG(16/14)
BsiEI 638 CGACCG CG[GA][CT]^CG
BspMI 98 ACCTGC ACCTGC(4/8)
BstEII 472 GGTCACC G^GT[ACGT]ACC
BstPI 472 GGTCACC G^GT[ACGT]ACC
BstYI 1221 AGATCT [GA]^GATC[CT]
CelII 279 GCTGAGC GC^T[ACGT]AGC
DsaI 713 CCGTGG C^C[GA][CT]GG
EcoO65I 472 GGTCACC G^GT[ACGT]ACC
HinfI 1024 GACTC G^A[ACGT]TC
KasI 300 GGCGCC G^GCGCC
MflI 1221 AGATCT [GA]^GATC[CT]
MluNI 230 TGGCCA TGG^CCA
MscI 230 TGGCCA TGG^CCA
NarI 300 GGCGCC GG^CGCC
PflFI 391 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1024 GACTC GAGTC(4/5)
RsaI 120 GTAC GT^AC
SacI 688 GAGCTC GAGCT^C
SbfI 99 CCTGCAGG CCTGCA^GG
ScaI 119 AGTACT AGT^ACT
SfoI 300 GGCGCC GGC^GCC
Sse8387I 99 CCTGCAGG CCTGCA^GG
Tth111I 391 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 734 CCAACTTCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1221 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaI 196 CCCGAG C^[CT]CG[GA]G
256 CCCGAG
AviII 239 TGCGCA TGC^GCA
462 TGCGCA
BanI 300 GGCGCC G^G[CT][GA]CC
761 GGCACC
BbsI 865 GAAGAC GAAGAC(2/6)
978 GAAGAC
BpuAI 865 GAAGAC GAAGAC(2/6)
978 GAAGAC
BseRI 822 CTCCTC GAGGAG(10/8)
157 GAGGAG
BsmAI 359 GTCTC GTCTC(1/5)
1228 GTCTC
BsoBI 196 CCCGAG C^[CT]CG[GA]G
256 CCCGAG
BsrFI 289 ACCGGC [GA]^CCGG[CT]
1058 ACCGGC
BstXI 623 CCATCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1254 CCAGCAACCTGG
Cfr10I 289 ACCGGC [GA]^CCGG[CT]
1058 ACCGGC
DpnII 1086 GATC ^GATC
1222 GATC
FspI 239 TGCGCA TGC^GCA
462 TGCGCA
HaeII 300 GGCGCC [GA]GCGC^[CT]
446 AGCGCC
HgaI 217 GCGTC GACGC(5/10)
680 GCGTC
MboI 1086 GATC ^GATC
1222 GATC
MspA1I 424 CCGCTG C[AC]G^C[GT]G
1080 CAGCGG
NdeII 1086 GATC ^GATC
1222 GATC
NlaIII 399 CATG CATG^
948 CATG
PpuMI 729 AGGACCC [GA]G^G[AT]CC[CT]
1208 AGGACCC
PstI 100 CTGCAG CTGCA^G
754 CTGCAG
Sau3AI 1086 GATC ^GATC
1222 GATC
SexAI 88 ACCTGGT A^CC[AT]GGT
392 ACCTGGT
SfcI 100 CTGCAG C^T[GA][CT]AG
754 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrBI BsrI
BssKI BssSI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MaeIII MboII MnlI MslI
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I