Restriction map for
pFN21AB6526
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
BalI BanI BbeI BbrPI BcgI
BciVI BclI BglI BsaAI BsaBI
BsaHI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CfoI
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccIII 494 TCCGGA T^CCGGA
AcsI 760 GAATTC [GA]^AATT[CT]
AflII 868 CTTAAG C^TTAAG
AflIII 160 ACATGT A^C[GA][CT]GT
AocI 268 CCTCAGG CC^T[ACGT]AGG
ApaI 274 GGGCCC GGGCC^C
ApoI 760 GAATTC [GA]^AATT[CT]
Asp700I 429 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 993 CCTAGG C^CTAGG
BamHI 938 GGATCC G^GATCC
BanII 274 GGGCCC G[GA]GC[CT]^C
BbsI 18 GAAGAC GAAGAC(2/6)
BbvCI 536 CCTCAGC CCTCAGC(-5/-2)
BcnI 913 CCGGG CC^[GC]GG
BfrI 868 CTTAAG C^TTAAG
BglII 219 AGATCT A^GATCT
BlnI 993 CCTAGG C^CTAGG
BlpI 571 GCTCAGC GC^T[ACGT]AGC
BmyI 274 GGGCCC G[AGT]GC[ACT]^C
BpmI 586 CTCCAG CTGGAG(16/14)
Bpu1102I 571 GCTCAGC GC^T[ACGT]AGC
BpuAI 18 GAAGAC GAAGAC(2/6)
BsaI 998 GAGACC GGTCTC(1/5)
BseAI 494 TCCGGA T^CCGGA
BseRI 454 GAGGAG GAGGAG(10/8)
Bsp1286I 274 GGGCCC G[AGT]GC[ACT]^C
BspEI 494 TCCGGA T^CCGGA
BspHI 1062 TCATGA T^CATGA
BspLU11I 160 ACATGT A^CATGT
BspMI 356 ACCTGC ACCTGC(4/8)
BsrI 855 ACTGG ACTGG(1/-1)
BstAPI 231 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 348 GGTGACC G^GT[ACGT]ACC
BstPI 348 GGTGACC G^GT[ACGT]ACC
BstXI 44 CCATCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 268 CCTCAGG CC^T[ACGT]AGG
CelII 571 GCTCAGC GC^T[ACGT]AGC
DraI 130 TTTAAA TTT^AAA
DrdI 100 GACGGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 618 CCACGG C^C[GA][CT]GG
EaeI 177 TGGCCG [CT]^GGCC[GA]
EarI 429 GAAGAG CTCTTC(1/4)
Eco81I 268 CCTCAGG CC^T[ACGT]AGG
EcoO65I 348 GGTGACC G^GT[ACGT]ACC
EcoRI 760 GAATTC G^AATTC
HphI 348 GGTGA GGTGA(8/7)
Ksp632I 429 GAAGAG CTCTTC(1/4)
MaeII 208 ACGT A^CGT
MroI 494 TCCGGA T^CCGGA
NciI 913 CCGGG CC^[GC]GG
NspI 160 ACATGT [GA]CATG^[CT]
PleI 962 GAGTC GAGTC(4/5)
PpuMI 384 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1062 TCATGA T^CATGA
ScaI 125 AGTACT AGT^ACT
SmlI 868 CTTAAG C^T[CT][GA]AG
StuI 590 AGGCCT AGG^CCT
TaqI 138 TCGA T^CGA
TfiI 256 GAATC G^A[AT]TC
TthHB8I 138 TCGA T^CGA
XmnI 429 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BsaWI 494 TCCGGA [AT]^CCGG[AT]
516 ACCGGA
BstYI 219 AGATCT [GA]^GATC[CT]
938 GGATCC
HinfI 256 GAATC G^A[ACGT]TC
962 GAGTC
MflI 219 AGATCT [GA]^GATC[CT]
938 GGATCC
MspA1I 251 CAGCTG C[AC]G^C[GT]G
831 CAGCTG
MwoI 231 GCAGACCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
297 GCGACTACTGC
PstI 340 CTGCAG CTGCA^G
376 CTGCAG
PvuII 251 CAGCTG CAG^CTG
831 CAGCTG
SfaNI 394 GATGC GCATC(5/9)
989 GCATC
Tsp45I 349 GTGAC ^GT[GC]AC
370 GTGAC
TspRI 374 CACTG CA[GC]TG[ACGT][ACGT]^
854 CACTG
XhoII 219 AGATCT [GA]^GATC[CT]
938 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BfaI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HpaII ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI StyI
Tru9I TseI Tsp509I