Restriction map for
pFN21AB9944
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI ApaI AscI AseI AsnI
Asp700I Asp718I AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BseAI BsiWI BsmI BspDI BspEI
BspHI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI DraI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT14I FbaI
FseI HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RcaI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
StuI StyI SwaI Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 1918 AAATTC [GA]^AATT[CT]
AhdI 1207 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1416 GTGCAC G^TGCAC
Aor51HI 965 AGCGCT AGC^GCT
ApaLI 1416 GTGCAC G^TGCAC
ApoI 1918 AAATTC [GA]^AATT[CT]
AspEI 1207 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 678 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1781 TGCGCA TGC^GCA
BglI 1321 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1384 TACGTG [CT]AC^GT[GA]
BsaI 165 GGTCTC GGTCTC(1/5)
BsiEI 1443 CGACCG CG[GA][CT]^CG
Bsp1407I 317 TGTACA T^GTACA
BspLU11I 1682 ACATGT A^CATGT
BsrGI 317 TGTACA T^GTACA
BstYI 1007 GGATCT [GA]^GATC[CT]
CpoI 1540 CGGACCG CG^G[AT]CCG
DraIII 340 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1468 GACCCGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1844 TGGCCG [CT]^GGCC[GA]
Eam1105I 1207 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 965 AGCGCT AGC^GCT
EcoNI 1393 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 873 ATGCAT ATGCA^T
FspI 1781 TGCGCA TGC^GCA
HaeII 965 AGCGCT [GA]GCGC^[CT]
MflI 1007 GGATCT [GA]^GATC[CT]
NaeI 1247 GCCGGC GCC^GGC
NgoMIV 1247 GCCGGC G^CCGGC
NsiI 873 ATGCAT ATGCA^T
PflFI 678 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 1540 CGGACCG CG^G[AT]CCG
SacI 1576 GAGCTC GAGCT^C
SbfI 553 CCTGCAGG CCTGCA^GG
SmaI 440 CCCGGG CCC^GGG
SmlI 1121 CTCAAG C^T[CT][GA]AG
SphI 923 GCATGC GCATG^C
Sse8387I 553 CCTGCAGG CCTGCA^GG
SspBI 317 TGTACA T^GTACA
TaqI 502 TCGA T^CGA
Tth111I 678 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 502 TCGA T^CGA
XcmI 152 CCAGCAATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1007 GGATCT [GA]^GATC[CT]
XmaCI 440 CCCGGG C^CCGGG
XmaI 440 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 504 GACGTC GACGT^C
823 GACGTC
AcyI 504 GACGTC G[GA]^CG[CT]C
823 GACGTC
AocI 96 CCTGAGG CC^T[ACGT]AGG
407 CCTCAGG
AspHI 1416 GTGCAC G[AT]GC[AT]^C
1576 GAGCTC
BbvCI 656 CCTCAGC CCTCAGC(-5/-2)
1483 CCTCAGC
BsaHI 504 GACGTC G[GA]^CG[CT]C
823 GACGTC
BsaWI 333 ACCGGA [AT]^CCGG[AT]
1445 ACCGGA
BsiHKAI 1416 GTGCAC G[AT]GC[AT]^C
1576 GAGCTC
BsmBI 126 GAGACG CGTCTC(1/5)
862 GAGACG
BsrBI 4 GAGCGG CCGCTC(-3/-3)
1003 GAGCGG
BsrFI 1247 GCCGGC [GA]^CCGG[CT]
1543 ACCGGC
BstAPI 875 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1418 GCACCTGCTGC
Bsu36I 96 CCTGAGG CC^T[ACGT]AGG
407 CCTCAGG
Cfr10I 1247 GCCGGC [GA]^CCGG[CT]
1543 ACCGGC
DsaI 539 CCACGG C^C[GA][CT]GG
998 CCGTGG
EarI 514 CTCTTC CTCTTC(1/4)
534 CTCTTC
Eco81I 96 CCTGAGG CC^T[ACGT]AGG
407 CCTCAGG
Hin1I 504 GACGTC G[GA]^CG[CT]C
823 GACGTC
HincII 1204 GTTGAC GT[CT]^[GA]AC
1686 GTTGAC
HindII 1204 GTTGAC GT[CT]^[GA]AC
1686 GTTGAC
Ksp632I 514 CTCTTC CTCTTC(1/4)
534 CTCTTC
TfiI 361 GAATC G^A[AT]TC
394 GAATC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PshAI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI Tru9I TseI Tsp45I
Tsp509I TspRI