Restriction map for
pFN21AE5507
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DpnII DraII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MboI MflI MluI MroI
MslI MspI MvnI NaeI NarI
NciI NcoI NdeII NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI Sau3AI Sau96I
SbfI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI StuI StyI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1180 GTAGAC GT^[AC][GT]AC
AflIII 771 ACACGT A^C[GA][CT]GT
Alw44I 1035 GTGCAC G^TGCAC
ApaLI 1035 GTGCAC G^TGCAC
BalI 683 TGGCCA TGG^CCA
BanII 1004 GAGCTC G[GA]GC[CT]^C
BfaI 131 CTAG C^TAG
BsaAI 1112 TACGTG [CT]AC^GT[GA]
BsaJI 369 CCTGGG C^C[ACGT][ACGT]GG
BseRI 1052 CTCCTC GAGGAG(10/8)
BsiYI 369 CCTGGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 369 CCTGGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI 852 CATTGC GCAATG(2/0)
BstAPI 847 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 1117 GCTGGC GC[ACGT]^[ACGT]GC
DraIII 587 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 683 TGGCCA [CT]^GGCC[GA]
HaeIII 684 GGCC GG^CC
HincII 468 GTTGAC GT[CT]^[GA]AC
HindII 468 GTTGAC GT[CT]^[GA]AC
HindIII 1205 AAGCTT A^AGCTT
HinfI 308 GAGTC G^A[ACGT]TC
HphI 592 GGTGA GGTGA(8/7)
MaeI 131 CTAG C^TAG
MfeI 332 CAATTG C^AATTG
MluNI 683 TGGCCA TGG^CCA
MscI 683 TGGCCA TGG^CCA
MunI 332 CAATTG C^AATTG
NdeI 439 CATATG CA^TATG
PleI 308 GAGTC GAGTC(4/5)
PstI 20 CTGCAG CTGCA^G
SacI 1004 GAGCTC GAGCT^C
ScaI 957 AGTACT AGT^ACT
SexAI 588 ACCAGGT A^CC[AT]GGT
SfcI 20 CTGCAG C^T[GA][CT]AG
SpeI 130 ACTAGT A^CTAGT
SspI 268 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 999 CCGC CCGC(-3/-1)
1152 CCGC
AlwNI 146 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
149 CAGCTGCTG
Asp700I 265 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
617 GAAGACTTTC
BbsI 617 GAAGAC GAAGAC(2/6)
837 GTCTTC
BpmI 239 CTCCAG CTGGAG(16/14)
512 CTGGAG
BpuAI 617 GAAGAC GAAGAC(2/6)
837 GTCTTC
BsmAI 932 GAGAC GTCTC(1/5)
310 GTCTC
DdeI 14 CTAAG C^T[ACGT]AG
327 CTCAG
DraI 34 TTTAAA TTT^AAA
216 TTTAAA
MaeII 773 ACGT A^CGT
1113 ACGT
MspA1I 149 CAGCTG C[AC]G^C[GT]G
397 CAGCTG
MwoI 292 GCTCTTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
847 GCAGACATTGC
NspI 596 ACATGC [GA]CATG^[CT]
880 GCATGT
PvuII 149 CAGCTG CAG^CTG
397 CAGCTG
XcmI 431 CCATTCATCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
671 CCATAGAACATTTGG
XmnI 265 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
617 GAAGACTTTC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
BbvI BmyI BsiHKAI Bsp1286I BsrI
BssKI BstF5I BstNI EcoRII Fnu4HI
FokI ItaI MaeIII MboII MnlI
MseI MvaI NlaIII RsaI ScrFI
SfaNI Tru9I TseI Tsp45I Tsp509I
TspRI