Restriction map for
pFN21AE1363
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbrPI BbsI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
KpnI KspI MamI MflI MluI
MluNI MroI MscI MvnI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 3 GGCGCC G[GA]^CG[CT]C
AgeI 1062 ACCGGT A^CCGGT
Alw44I 578 GTGCAC G^TGCAC
ApaLI 578 GTGCAC G^TGCAC
AseI 508 ATTAAT AT^TAAT
AsnI 508 ATTAAT AT^TAAT
Asp700I 360 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 3 GGCGCC GGCGC^C
BcnI 1098 CCCGG CC^[GC]GG
BpmI 1723 CTCCAG CTGGAG(16/14)
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsaWI 1062 ACCGGT [AT]^CCGG[AT]
BsgI 208 CTGCAC GTGCAG(16/14)
BsmAI 1567 GTCTC GTCTC(1/5)
Bsp1407I 47 TGTACA T^GTACA
BspMI 1227 GCAGGT ACCTGC(4/8)
BsrBI 16 CCGCTC CCGCTC(-3/-3)
BsrFI 1062 ACCGGT [GA]^CCGG[CT]
BsrGI 47 TGTACA T^GTACA
CfoI 4 GCGC GCG^C
Cfr10I 1062 ACCGGT [GA]^CCGG[CT]
EarI 1387 GAAGAG CTCTTC(1/4)
EcoT14I 94 CCAAGG C^C[AT][AT]GG
FauI 428 CCCGC CCCGC(4/6)
HaeII 3 GGCGCC [GA]GCGC^[CT]
HaeIII 820 GGCC GG^CC
HhaI 4 GCGC GCG^C
Hin1I 3 GGCGCC G[GA]^CG[CT]C
HinP1I 4 GCGC G^CGC
HpaI 436 GTTAAC GTT^AAC
KasI 3 GGCGCC G^GCGCC
Ksp632I 1387 GAAGAG CTCTTC(1/4)
MaeII 274 ACGT A^CGT
MfeI 479 CAATTG C^AATTG
MslI 1192 CACAAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 308 CAGCTG C[AC]G^C[GT]G
MunI 479 CAATTG C^AATTG
NarI 3 GGCGCC GG^CGCC
NciI 1098 CCCGG CC^[GC]GG
NheI 715 GCTAGC G^CTAGC
PinAI 1062 ACCGGT A^CCGGT
PleI 1515 GAGTC GAGTC(4/5)
PshAI 1510 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 508 ATTAAT AT^TAAT
PvuII 308 CAGCTG CAG^CTG
SfoI 3 GGCGCC GGC^GCC
SspBI 47 TGTACA T^GTACA
StyI 94 CCAAGG C^C[AT][AT]GG
XmnI 360 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 16 CCGC CCGC(-3/-1)
429 CCGC
AflIII 352 ACATGT A^C[GA][CT]GT
1126 ACATGT
AlwI 919 GATCC GGATC(4/5)
34 GGATC
AlwNI 1661 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1726 CAGGAGCTG
BciVI 314 GGATAC GTATCC(6/5)
1156 GGATAC
BspLU11I 352 ACATGT A^CATGT
1126 ACATGT
DpnII 35 GATC ^GATC
919 GATC
DraII 142 GGGACCT [GA]G^G[ACGT]CC[CT]
1586 GGGACCC
EcoNI 222 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1719 CCTTCTCCAGG
EcoO109I 142 GGGACCT [GA]G^G[ACGT]CC[CT]
1586 GGGACCC
EcoRI 244 GAATTC G^AATTC
843 GAATTC
HapII 1063 CCGG C^CGG
1099 CCGG
HincII 436 GTTAAC GT[CT]^[GA]AC
727 GTTGAC
HindII 436 GTTAAC GT[CT]^[GA]AC
727 GTTGAC
HpaII 1063 CCGG C^CGG
1099 CCGG
MboI 35 GATC ^GATC
919 GATC
MspI 1063 CCGG C^CGG
1099 CCGG
NdeII 35 GATC ^GATC
919 GATC
NspI 352 ACATGT [GA]CATG^[CT]
1126 ACATGT
PflMI 811 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1725 CCAGGAGCTGG
PpuMI 142 GGGACCT [GA]G^G[AT]CC[CT]
1586 GGGACCC
Sau3AI 35 GATC ^GATC
919 GATC
SfcI 797 CTGTAG C^T[GA][CT]AG
963 CTATAG
SspI 178 AATATT AAT^ATT
1257 AATATT
TaqI 218 TCGA T^CGA
1640 TCGA
TfiI 100 GAATC G^A[AT]TC
1075 GATTC
Tsp45I 190 GTCAC ^GT[GC]AC
1508 GTGAC
TthHB8I 218 TCGA T^CGA
1640 TCGA
Van91I 811 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1725 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
AvaII BanI BbvI BfaI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI FokI HinfI HphI ItaI
MaeI MaeIII MboII MnlI MseI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI Tru9I TseI Tsp509I
TspRI