Restriction map for
pFN21AE5306
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I EarI Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI Hin1I HindIII
HpaI HphI KasI Ksp632I KspI
MaeI MamI MboII MfeI MluI
MluNI MroI MscI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 134 GGTACC G^GTACC
Alw44I 532 GTGCAC G^TGCAC
AlwI 475 GATCC GGATC(4/5)
ApaLI 532 GTGCAC G^TGCAC
Asp718I 134 GGTACC G^GTACC
AspHI 532 GTGCAC G[AT]GC[AT]^C
AviII 638 TGCGCA TGC^GCA
BanI 134 GGTACC G^G[CT][GA]CC
BglII 438 AGATCT A^GATCT
BpmI 711 CTGGAG CTGGAG(16/14)
BseRI 845 CTCCTC GAGGAG(10/8)
BsiHKAI 532 GTGCAC G[AT]GC[AT]^C
BsmFI 74 GGGAC GGGAC(10/14)
BsrDI 302 CATTGC GCAATG(2/0)
BsrI 71 ACTGG ACTGG(1/-1)
BstEII 333 GGTAACC G^GT[ACGT]ACC
BstPI 333 GGTAACC G^GT[ACGT]ACC
BstXI 345 CCATGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 438 AGATCT [GA]^GATC[CT]
EcoNI 231 CCTAAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 333 GGTAACC G^GT[ACGT]ACC
EcoT14I 287 CCAAGG C^C[AT][AT]GG
FseI 838 GGCCGGCC GGCCGG^CC
FspI 638 TGCGCA TGC^GCA
HaeII 216 GGCGCT [GA]GCGC^[CT]
HgaI 918 GACGC GACGC(5/10)
HincII 61 GTCAAC GT[CT]^[GA]AC
HindII 61 GTCAAC GT[CT]^[GA]AC
HinfI 108 GACTC G^A[ACGT]TC
KpnI 134 GGTACC GGTAC^C
MaeII 77 ACGT A^CGT
MflI 438 AGATCT [GA]^GATC[CT]
MslI 535 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 108 GACTC GAGTC(4/5)
StuI 755 AGGCCT AGG^CCT
StyI 287 CCAAGG C^C[AT][AT]GG
TaqI 395 TCGA T^CGA
Tsp45I 283 GTGAC ^GT[GC]AC
TthHB8I 395 TCGA T^CGA
XcmI 287 CCAAGGCTTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 438 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 600 GGACC G^G[AT]CC
741 GGACC
BglI 757 GCCTTACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
834 GCCCGGCCGGC
BsaWI 38 ACCGGA [AT]^CCGG[AT]
927 ACCGGA
BsiEI 604 CGGCCG CG[GA][CT]^CG
837 CGGCCG
BspMI 166 ACCTGC ACCTGC(4/8)
635 ACCTGC
BsrBI 502 CCGCTC CCGCTC(-3/-3)
274 GAGCGG
BssSI 442 CTCGTG CACGAG(-5/-1)
748 CTCGTG
DdeI 869 CTGAG C^T[ACGT]AG
875 CTGAG
DpnII 439 GATC ^GATC
475 GATC
DsaI 557 CCGTGG C^C[GA][CT]GG
647 CCACGG
EagI 604 CGGCCG C^GGCCG
837 CGGCCG
EclXI 604 CGGCCG C^GGCCG
837 CGGCCG
Eco52I 604 CGGCCG C^GGCCG
837 CGGCCG
MaeIII 283 GTGAC ^GT[ACGT]AC
334 GTAAC
MboI 439 GATC ^GATC
475 GATC
NaeI 247 GCCGGC GCC^GGC
839 GCCGGC
NdeII 439 GATC ^GATC
475 GATC
NgoMIV 247 GCCGGC G^CCGGC
839 GCCGGC
NlaIII 346 CATG CATG^
387 CATG
PflMI 189 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
552 CCACCCCGTGG
Sau3AI 439 GATC ^GATC
475 GATC
SfiI 756 GGCCTTACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
833 GGCCCGGCCGGCC
Van91I 189 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
552 CCACCCCGTGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AvaI BanII BbvI BcnI
BmyI BsaJI BsgI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DraII DraIII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV PstI
PvuII RsaI Sau96I ScrFI SfaNI
SfcI TseI TspRI