Restriction map for
pFN21AE1315
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfrI BlnI BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I MamI
MboII MfeI MluI MroI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 703 GTAGAC GT^[AC][GT]AC
AlwI 126 GATCC GGATC(4/5)
Aor51HI 754 AGCGCT AGC^GCT
AspI 706 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 759 TGGCCA TGG^CCA
BciVI 102 GGATAC GTATCC(6/5)
BfaI 361 CTAG C^TAG
BglII 81 AGATCT A^GATCT
BlpI 574 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 574 GCTGAGC GC^T[ACGT]AGC
BsaWI 116 TCCGGT [AT]^CCGG[AT]
BsmFI 532 GGGAC GGGAC(10/14)
BspHI 626 TCATGA T^CATGA
BsrDI 495 CATTGC GCAATG(2/0)
BsrFI 250 GCCGGC [GA]^CCGG[CT]
BssHII 153 GCGCGC G^CGCGC
BstEII 582 GGTGACC G^GT[ACGT]ACC
BstPI 582 GGTGACC G^GT[ACGT]ACC
BstXI 407 CCACGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 574 GCTGAGC GC^T[ACGT]AGC
Cfr10I 250 GCCGGC [GA]^CCGG[CT]
EaeI 759 TGGCCA [CT]^GGCC[GA]
Eco47III 754 AGCGCT AGC^GCT
EcoNI 594 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 582 GGTGACC G^GT[ACGT]ACC
HincII 565 GTCAAC GT[CT]^[GA]AC
HindII 565 GTCAAC GT[CT]^[GA]AC
KspI 177 CCGCGG CCGC^GG
MaeI 361 CTAG C^TAG
MluNI 759 TGGCCA TGG^CCA
MscI 759 TGGCCA TGG^CCA
NaeI 250 GCCGGC GCC^GGC
NcoI 203 CCATGG C^CATGG
NgoMIV 250 GCCGGC G^CCGGC
NspI 336 GCATGC [GA]CATG^[CT]
PflFI 706 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 532 GGGACCT [GA]G^G[AT]CC[CT]
PstI 256 CTGCAG CTGCA^G
RcaI 626 TCATGA T^CATGA
SacII 177 CCGCGG CCGC^GG
SmaI 443 CCCGGG CCC^GGG
SphI 336 GCATGC GCATG^C
Tth111I 706 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 443 CCCGGG C^CCGGG
XmaI 443 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 154 CGCG CG^CG
178 CGCG
ApaI 219 GGGCCC GGGCC^C
793 GGGCCC
AvaII 423 GGTCC G^G[AT]CC
533 GGACC
BanI 190 GGCACC G^G[CT][GA]CC
379 GGTGCC
BglI 661 GCCTTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
889 GCCCTCATGGC
BpmI 781 CTCCAG CTGGAG(16/14)
449 CTGGAG
BsgI 678 CTGCAC GTGCAG(16/14)
903 CTGCAC
BstF5I 411 GGATG GGATG(2/0)
603 GGATG
BstUI 154 CGCG CG^CG
178 CGCG
BstYI 81 AGATCT [GA]^GATC[CT]
125 AGATCC
DpnII 82 GATC ^GATC
126 GATC
FokI 411 GGATG GGATG(9/13)
603 GGATG
HinfI 301 GATTC G^A[ACGT]TC
428 GATTC
MaeII 707 ACGT A^CGT
851 ACGT
MboI 82 GATC ^GATC
126 GATC
MflI 81 AGATCT [GA]^GATC[CT]
125 AGATCC
MslI 18 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
408 CACGGATGTG
MvnI 154 CGCG CG^CG
178 CGCG
NdeII 82 GATC ^GATC
126 GATC
PvuII 65 CAGCTG CAG^CTG
877 CAGCTG
Sau3AI 82 GATC ^GATC
126 GATC
TfiI 301 GATTC G^A[AT]TC
428 GATTC
Tsp45I 583 GTGAC ^GT[GC]AC
865 GTCAC
XcmI 194 CCATTCTGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
374 CCACAGGTGCCCTGG
XhoII 81 AGATCT [GA]^GATC[CT]
125 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AvaI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsoBI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SfcI StyI TseI
TspRI