Restriction map for
pFN21AE1567
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AvrII BbeI
BbsI BbvCI BciVI BfrI BglII
BlnI BpuAI BsaBI BsaHI BseAI
BseRI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FseI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 149 ACACGT A^C[GA][CT]GT
Alw44I 384 GTGCAC G^TGCAC
ApaI 737 GGGCCC GGGCC^C
ApaLI 384 GTGCAC G^TGCAC
AspHI 384 GTGCAC G[AT]GC[AT]^C
AspI 346 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 233 TGCGCA TGC^GCA
BamHI 362 GGATCC G^GATCC
BanI 1037 GGTGCC G^G[CT][GA]CC
BcgI 332 GCAAGGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 275 TGATCA T^GATCA
BglI 832 GCCGAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1131 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1131 GCTGAGC GC^T[ACGT]AGC
BsaWI 359 ACCGGA [AT]^CCGG[AT]
BsgI 1111 GTGCAG GTGCAG(16/14)
BsiEI 536 CGGCCG CG[GA][CT]^CG
BsiHKAI 384 GTGCAC G[AT]GC[AT]^C
Bsp1407I 953 TGTACA T^GTACA
BsrBI 463 GAGCGG CCGCTC(-3/-3)
BsrDI 50 GCAATG GCAATG(2/0)
BsrGI 953 TGTACA T^GTACA
BssSI 847 CACGAG CACGAG(-5/-1)
BstAPI 50 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1131 GCTGAGC GC^T[ACGT]AGC
EagI 536 CGGCCG C^GGCCG
EclXI 536 CGGCCG C^GGCCG
Eco52I 536 CGGCCG C^GGCCG
EcoNI 1101 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1106 CCAAGG C^C[AT][AT]GG
FbaI 275 TGATCA T^GATCA
FspI 233 TGCGCA TGC^GCA
HgaI 660 GCGTC GACGC(5/10)
MaeIII 914 GTGAC ^GT[ACGT]AC
MslI 534 CACGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 633 CATATG CA^TATG
PflFI 346 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 631 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 394 CTGCAG CTGCA^G
SapI 255 GCTCTTC GCTCTTC(1/4)
SexAI 347 ACCTGGT A^CC[AT]GGT
SphI 1127 GCATGC GCATG^C
SspBI 953 TGTACA T^GTACA
StyI 1106 CCAAGG C^C[AT][AT]GG
TfiI 1216 GATTC G^A[AT]TC
Tsp45I 914 GTGAC ^GT[GC]AC
Tth111I 346 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 533 CCACGGCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 343 GTCGAC GT^[AC][GT]AC
610 GTCGAC
AccII 987 CGCG CG^CG
1152 CGCG
BalI 806 TGGCCA TGG^CCA
1103 TGGCCA
BbrPI 22 CACGTG CAC^GTG
181 CACGTG
BcnI 281 CCGGG CC^[GC]GG
819 CCCGG
BfaI 499 CTAG C^TAG
775 CTAG
BsaI 1097 GAGACC GGTCTC(1/5)
528 GGTCTC
BstUI 987 CGCG CG^CG
1152 CGCG
BstYI 362 GGATCC [GA]^GATC[CT]
378 GGATCT
DraII 736 GGGGCCC [GA]G^G[ACGT]CC[CT]
737 GGGCCCT
EarI 256 CTCTTC CTCTTC(1/4)
1201 GAAGAG
EcoO109I 736 GGGGCCC [GA]G^G[ACGT]CC[CT]
737 GGGCCCT
EcoRI 86 GAATTC G^AATTC
760 GAATTC
FauI 788 CCCGC CCCGC(4/6)
1116 GCGGG
Ksp632I 256 CTCTTC CTCTTC(1/4)
1201 GAAGAG
MaeI 499 CTAG C^TAG
775 CTAG
MflI 362 GGATCC [GA]^GATC[CT]
378 GGATCT
MluNI 806 TGGCCA TGG^CCA
1103 TGGCCA
MscI 806 TGGCCA TGG^CCA
1103 TGGCCA
MseI 1052 TTAA T^TAA
1064 TTAA
MvnI 987 CGCG CG^CG
1152 CGCG
NciI 281 CCGGG CC^[GC]GG
819 CCCGG
NspI 947 GCATGT [GA]CATG^[CT]
1127 GCATGC
PleI 139 GACTC GAGTC(4/5)
38 GAGTC
PmaCI 22 CACGTG CAC^GTG
181 CACGTG
PmlI 22 CACGTG CAC^GTG
181 CACGTG
PvuII 271 CAGCTG CAG^CTG
853 CAGCTG
SalI 343 GTCGAC G^TCGAC
610 GTCGAC
SfcI 246 CTATAG C^T[GA][CT]AG
394 CTGCAG
StuI 839 AGGCCT AGG^CCT
1157 AGGCCT
Tru9I 1052 TTAA T^TAA
1064 TTAA
XhoII 362 GGATCC [GA]^GATC[CT]
378 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbvI BmyI
BpmI BsaAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp509I TspRI TthHB8I