Restriction map for
pFN21AE0732
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbrPI BbsI BbvCI BcgI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HgaI HincII HindII HpaI KpnI
MamI MfeI MluI MroI MslI
MunI NaeI NdeI NgoMIV NruI
NsiI NspI NspV PacI PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 523 GTAGAC GT^[AC][GT]AC
AcsI 391 GAATTC [GA]^AATT[CT]
AcyI 242 GGCGCC G[GA]^CG[CT]C
AlwNI 548 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 167 GGGCCC GGGCC^C
ApoI 391 GAATTC [GA]^AATT[CT]
AscI 159 GGCGCGCC GG^CGCGCC
AspHI 1200 GAGCAC G[AT]GC[AT]^C
AvaI 1220 CTCGAG C^[CT]CG[GA]G
AvaII 6 GGTCC G^G[AT]CC
BalI 1191 TGGCCA TGG^CCA
BamHI 403 GGATCC G^GATCC
BanI 242 GGCGCC G^G[CT][GA]CC
BbeI 242 GGCGCC GGCGC^C
BglI 99 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 486 AGATCT A^GATCT
BsaHI 242 GGCGCC G[GA]^CG[CT]C
BseRI 121 CTCCTC GAGGAG(10/8)
BsgI 528 CTGCAC GTGCAG(16/14)
BsiHKAI 1200 GAGCAC G[AT]GC[AT]^C
BsmAI 504 GTCTC GTCTC(1/5)
BsmFI 7 GTCCC GGGAC(10/14)
BsoBI 1220 CTCGAG C^[CT]CG[GA]G
BstNI 14 CCAGG CC^[AT]GG
BstXI 885 CCATCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 5 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 343 GACAGCTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 5 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 391 GAATTC G^AATTC
EcoRII 14 CCAGG ^CC[AT]GG
Hin1I 242 GGCGCC G[GA]^CG[CT]C
HindIII 671 AAGCTT A^AGCTT
HinfI 426 GAATC G^A[ACGT]TC
KasI 242 GGCGCC G^GCGCC
MaeII 179 ACGT A^CGT
MluNI 1191 TGGCCA TGG^CCA
MscI 1191 TGGCCA TGG^CCA
MvaI 14 CCAGG CC^[AT]GG
NarI 242 GGCGCC GG^CGCC
NcoI 794 CCATGG C^CATGG
NheI 111 GCTAGC G^CTAGC
PaeR7I 1220 CTCGAG C^TCGAG
PflMI 547 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 5 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 980 CTGCAG CTGCA^G
PvuII 983 CAGCTG CAG^CTG
SapI 850 GAAGAGC GCTCTTC(1/4)
ScaI 497 AGTACT AGT^ACT
SfoI 242 GGCGCC GGC^GCC
TfiI 426 GAATC G^A[AT]TC
Van91I 547 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1220 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BanII 167 GGGCCC G[GA]GC[CT]^C
720 GAGCCC
BclI 1062 TGATCA T^GATCA
1101 TGATCA
BfaI 112 CTAG C^TAG
599 CTAG
BsiEI 91 CGGCCG CG[GA][CT]^CG
97 CGGCCG
BsrBI 50 CCGCTC CCGCTC(-3/-3)
118 CCGCTC
BssHII 160 GCGCGC G^CGCGC
322 GCGCGC
BstF5I 435 GGATG GGATG(2/0)
639 GGATG
DraI 268 TTTAAA TTT^AAA
675 TTTAAA
EagI 91 CGGCCG C^GGCCG
97 CGGCCG
EclXI 91 CGGCCG C^GGCCG
97 CGGCCG
Eco52I 91 CGGCCG C^GGCCG
97 CGGCCG
EcoT14I 794 CCATGG C^C[AT][AT]GG
1041 CCAAGG
FbaI 1062 TGATCA T^GATCA
1101 TGATCA
FokI 435 GGATG GGATG(9/13)
639 GGATG
HaeII 173 AGCGCC [GA]GCGC^[CT]
242 GGCGCC
KspI 94 CCGCGG CCGC^GG
103 CCGCGG
MaeI 112 CTAG C^TAG
599 CTAG
NlaIII 25 CATG CATG^
795 CATG
NotI 90 GCGGCCGC GC^GGCCGC
96 GCGGCCGC
SacII 94 CCGCGG CCGC^GG
103 CCGCGG
SfaNI 592 GATGC GCATC(5/9)
1216 GCATC
SmlI 462 CTTGAG C^T[CT][GA]AG
1220 CTCGAG
SspI 752 AATATT AAT^ATT
781 AATATT
StyI 794 CCATGG C^C[AT][AT]GG
1041 CCAAGG
TaqI 507 TCGA T^CGA
1221 TCGA
TspRI 552 CACTG CA[GC]TG[ACGT][ACGT]^
1188 CAGTG
TthHB8I 507 TCGA T^CGA
1221 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BciVI BcnI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstUI BstYI Cac8I CfoI Cfr13I
DpnII DsaI EaeI EarI FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvnI MwoI NciI
NdeII NlaIV RsaI Sau3AI Sau96I
ScrFI SfcI Tru9I TseI Tsp45I
Tsp509I XhoII