Restriction map for
pFN21AE5601
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaBI
BsaI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eam1105I
EarI Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MamI MfeI MluI MroI
MseI MunI NheI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 1123 GTCTAC GT^[AC][GT]AC
AcsI 1038 GAATTT [GA]^AATT[CT]
AfaI 231 GTAC GT^AC
AflIII 348 ACACGT A^C[GA][CT]GT
ApoI 1038 GAATTT [GA]^AATT[CT]
BamHI 516 GGATCC G^GATCC
BbrPI 349 CACGTG CAC^GTG
BbsI 344 GAAGAC GAAGAC(2/6)
BpuAI 344 GAAGAC GAAGAC(2/6)
BsaAI 349 CACGTG [CT]AC^GT[GA]
BsaWI 584 ACCGGA [AT]^CCGG[AT]
BsrBI 1320 CCGCTC CCGCTC(-3/-3)
BsrDI 905 GCAATG GCAATG(2/0)
BsrFI 284 GCCGGC [GA]^CCGG[CT]
BstEII 802 GGTGACC G^GT[ACGT]ACC
BstPI 802 GGTGACC G^GT[ACGT]ACC
Cfr10I 284 GCCGGC [GA]^CCGG[CT]
DrdI 588 GACATATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 522 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 802 GGTGACC G^GT[ACGT]ACC
KspI 153 CCGCGG CCGC^GG
MslI 815 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 284 GCCGGC GCC^GGC
NdeI 590 CATATG CA^TATG
NgoMIV 284 GCCGGC G^CCGGC
NotI 197 GCGGCCGC GC^GGCCGC
PmaCI 349 CACGTG CAC^GTG
PmlI 349 CACGTG CAC^GTG
PpuMI 937 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1353 CAGCTG CAG^CTG
RsaI 231 GTAC GT^AC
SacII 153 CCGCGG CCGC^GG
SfaNI 1395 GCATC GCATC(5/9)
SmaI 42 CCCGGG CCC^GGG
SphI 1223 GCATGC GCATG^C
Tsp509I 1039 AATT ^AATT
XmaCI 42 CCCGGG C^CCGGG
XmaI 42 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AvaI 42 CCCGGG C^[CT]CG[GA]G
71 CCCGAG
BlpI 431 GCTCAGC GC^T[ACGT]AGC
1146 GCTGAGC
Bpu1102I 431 GCTCAGC GC^T[ACGT]AGC
1146 GCTGAGC
BsiEI 156 CGGCCG CG[GA][CT]^CG
198 CGGCCG
BsmAI 481 GAGAC GTCTC(1/5)
1054 GAGAC
BsoBI 42 CCCGGG C^[CT]CG[GA]G
71 CCCGAG
BstXI 791 CCAAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
814 CCACCTGAATGG
CelII 431 GCTCAGC GC^T[ACGT]AGC
1146 GCTGAGC
EagI 156 CGGCCG C^GGCCG
198 CGGCCG
EclXI 156 CGGCCG C^GGCCG
198 CGGCCG
Eco52I 156 CGGCCG C^GGCCG
198 CGGCCG
HgaI 171 GACGC GACGC(5/10)
203 GCGTC
MaeII 350 ACGT A^CGT
1328 ACGT
MspA1I 153 CCGCGG C[AC]G^C[GT]G
1353 CAGCTG
NcoI 275 CCATGG C^CATGG
1427 CCATGG
SacI 337 GAGCTC GAGCT^C
964 GAGCTC
StuI 89 AGGCCT AGG^CCT
992 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaII BalI BanI
BanII BbeI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeIII MboI MboII MflI
MluNI MnlI MscI MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI Sau3AI Sau96I ScrFI SfcI
SfoI StyI TseI Tsp45I TspRI
Van91I XcmI XhoII