Restriction map for
pFN21AB6554
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BpmI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FseI FspI HapII HgaI
Hin1I HindIII HpaII KasI KpnI
Ksp632I KspI MamI MluI MroI
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 610 GTAGAC GT^[AC][GT]AC
AlwI 796 GATCC GGATC(4/5)
AocI 883 CCTGAGG CC^T[ACGT]AGG
Asp700I 998 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 59 CTCGAG C^[CT]CG[GA]G
BalI 552 TGGCCA TGG^CCA
BfaI 1010 CTAG C^TAG
BlpI 1165 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1165 GCTCAGC GC^T[ACGT]AGC
BsaAI 343 CACGTA [CT]AC^GT[GA]
BseRI 266 GAGGAG GAGGAG(10/8)
BsoBI 59 CTCGAG C^[CT]CG[GA]G
BsrDI 952 GCAATG GCAATG(2/0)
BstBI 125 TTCGAA TT^CGAA
BstXI 737 CCAAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 795 AGATCC [GA]^GATC[CT]
Bsu36I 883 CCTGAGG CC^T[ACGT]AGG
CelII 1165 GCTCAGC GC^T[ACGT]AGC
DsaI 699 CCATGG C^C[GA][CT]GG
EaeI 552 TGGCCA [CT]^GGCC[GA]
Eco81I 883 CCTGAGG CC^T[ACGT]AGG
EcoNI 1096 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 811 GATATC GAT^ATC
FauI 325 CCCGC CCCGC(4/6)
HincII 381 GTTAAC GT[CT]^[GA]AC
HindII 381 GTTAAC GT[CT]^[GA]AC
HpaI 381 GTTAAC GTT^AAC
MaeI 1010 CTAG C^TAG
MfeI 367 CAATTG C^AATTG
MflI 795 AGATCC [GA]^GATC[CT]
MluNI 552 TGGCCA TGG^CCA
MscI 552 TGGCCA TGG^CCA
MunI 367 CAATTG C^AATTG
NcoI 699 CCATGG C^CATGG
NspV 125 TTCGAA TT^CGAA
PaeR7I 59 CTCGAG C^TCGAG
PleI 79 GAGTC GAGTC(4/5)
SfaNI 1140 GATGC GCATC(5/9)
SfuI 125 TTCGAA TT^CGAA
SphI 1013 GCATGC GCATG^C
StuI 887 AGGCCT AGG^CCT
TfiI 417 GATTC G^A[AT]TC
XhoI 59 CTCGAG C^TCGAG
XhoII 795 AGATCC [GA]^GATC[CT]
XmnI 998 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 326 CCGC CCGC(-3/-1)
1157 CCGC
AcsI 203 AAATTC [GA]^AATT[CT]
258 GAATTT
AhdI 307 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
922 GACCACCAGTC
ApoI 203 AAATTC [GA]^AATT[CT]
258 GAATTT
AspEI 307 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
922 GACCACCAGTC
AspHI 499 GAGCAC G[AT]GC[AT]^C
958 GAGCAC
BclI 492 TGATCA T^GATCA
717 TGATCA
BmyI 499 GAGCAC G[AGT]GC[ACT]^C
958 GAGCAC
BsiHKAI 499 GAGCAC G[AT]GC[AT]^C
958 GAGCAC
BsiYI 324 CCCCGCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1096 CCTCTGGCAGG
BslI 324 CCCCGCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1096 CCTCTGGCAGG
Bsp1286I 499 GAGCAC G[AGT]GC[ACT]^C
958 GAGCAC
BspHI 485 TCATGA T^CATGA
495 TCATGA
BspLU11I 84 ACATGT A^CATGT
407 ACATGT
BstF5I 547 CATCC GGATG(2/0)
1139 GGATG
CfoI 1043 GCGC GCG^C
1163 GCGC
Eam1105I 307 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
922 GACCACCAGTC
FbaI 492 TGATCA T^GATCA
717 TGATCA
FokI 547 CATCC GGATG(9/13)
1139 GGATG
HaeII 1042 AGCGCC [GA]GCGC^[CT]
1162 GGCGCT
HhaI 1043 GCGC GCG^C
1163 GCGC
HinP1I 1043 GCGC G^CGC
1163 GCGC
HinfI 79 GAGTC G^A[ACGT]TC
417 GATTC
PvuII 118 CAGCTG CAG^CTG
1151 CAGCTG
RcaI 485 TCATGA T^CATGA
495 TCATGA
SmlI 59 CTCGAG C^T[CT][GA]AG
208 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AfaI AflIII AluI AvaII BbvI
BsaJI BsmFI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PpuMI RsaI Sau3AI Sau96I
ScrFI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I