Restriction map for
pFN21AE4419
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AviII AvrII BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsiHKAI BsiWI BsoBI Bsp1407I
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 712 CGCG CG^CG
AgeI 977 ACCGGT A^CCGGT
ApaI 367 GGGCCC GGGCC^C
AspI 544 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 503 TGGCCA TGG^CCA
BamHI 512 GGATCC G^GATCC
BbvCI 214 GCTGAGG CCTCAGC(-5/-2)
BsiEI 179 CGGCCG CG[GA][CT]^CG
BsmBI 175 GAGACG CGTCTC(1/5)
BsmI 287 GCATTC GAATGC(1/-1)
BspDI 916 ATCGAT AT^CGAT
BspMI 969 GCAGGT ACCTGC(4/8)
BsrBI 109 GAGCGG CCGCTC(-3/-3)
BstAPI 432 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 774 GGTGACC G^GT[ACGT]ACC
BstPI 774 GGTGACC G^GT[ACGT]ACC
BstUI 712 CGCG CG^CG
BstXI 1160 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 512 GGATCC [GA]^GATC[CT]
ClaI 916 ATCGAT AT^CGAT
EagI 179 CGGCCG C^GGCCG
EclXI 179 CGGCCG C^GGCCG
Eco52I 179 CGGCCG C^GGCCG
EcoO65I 774 GGTGACC G^GT[ACGT]ACC
MaeII 908 ACGT A^CGT
MflI 512 GGATCC [GA]^GATC[CT]
MluNI 503 TGGCCA TGG^CCA
MscI 503 TGGCCA TGG^CCA
MspA1I 231 CAGCTG C[AC]G^C[GT]G
MvnI 712 CGCG CG^CG
NspI 1253 GCATGT [GA]CATG^[CT]
PflFI 544 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 977 ACCGGT A^CCGGT
PstI 748 CTGCAG CTGCA^G
PvuII 231 CAGCTG CAG^CTG
SexAI 1091 ACCAGGT A^CC[AT]GGT
SfcI 748 CTGCAG C^T[GA][CT]AG
SmlI 517 CTCAAG C^T[CT][GA]AG
StuI 791 AGGCCT AGG^CCT
TfiI 381 GATTC G^A[AT]TC
Tth111I 544 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1088 CCAACCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 512 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 139 GGCGCC G[GA]^CG[CT]C
998 GGCGCC
BanII 367 GGGCCC G[GA]GC[CT]^C
707 GAGCCC
BbeI 139 GGCGCC GGCGC^C
998 GGCGCC
BcnI 88 CCGGG CC^[GC]GG
585 CCGGG
BsaHI 139 GGCGCC G[GA]^CG[CT]C
998 GGCGCC
BsaI 193 GAGACC GGTCTC(1/5)
466 GAGACC
BsaWI 291 TCCGGT [AT]^CCGG[AT]
977 ACCGGT
BseRI 322 GAGGAG GAGGAG(10/8)
463 GAGGAG
BsgI 160 CTGCAC GTGCAG(16/14)
220 GTGCAG
BsmFI 51 GGGAC GGGAC(10/14)
591 GTCCC
BsrDI 69 CATTGC GCAATG(2/0)
273 CATTGC
BsrI 475 ACTGG ACTGG(1/-1)
1242 CCAGT
HaeII 139 GGCGCC [GA]GCGC^[CT]
998 GGCGCC
HgaI 53 GACGC GACGC(5/10)
713 GCGTC
Hin1I 139 GGCGCC G[GA]^CG[CT]C
998 GGCGCC
HincII 1033 GTCAAC GT[CT]^[GA]AC
1215 GTTGAC
HindII 1033 GTCAAC GT[CT]^[GA]AC
1215 GTTGAC
KasI 139 GGCGCC G^GCGCC
998 GGCGCC
MboII 990 GAAGA GAAGA(8/7)
242 TCTTC
NaeI 634 GCCGGC GCC^GGC
851 GCCGGC
NarI 139 GGCGCC GG^CGCC
998 GGCGCC
NciI 88 CCGGG CC^[GC]GG
585 CCGGG
NgoMIV 634 GCCGGC G^CCGGC
851 GCCGGC
PflMI 1092 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1242 CCAGTACCTGG
PleI 544 GACTC GAGTC(4/5)
589 GAGTC
SfoI 139 GGCGCC GGC^GCC
998 GGCGCC
TaqI 917 TCGA T^CGA
1109 TCGA
TthHB8I 917 TCGA T^CGA
1109 TCGA
Van91I 1092 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1242 CCAGTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbvI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrFI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MnlI MslI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PshAI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI