Restriction map for
pFN21AE5066
##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccII AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspI AvrII
BamHI BcgI BclI BfaI BfrI
BlnI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FbaI
HindIII HpaI KpnI KspI MaeI
MluI MvnI NdeI NheI NotI
NruI NspV PacI PflFI PinAI
PmeI PshAI PvuI RsrII SacII
SalI ScaI SfuI SgrAI SmaI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 1306 GTAGAC GT^[AC][GT]AC
AccIII 2246 TCCGGA T^CCGGA
AclI 2830 AACGTT AA^CGTT
AfaI 1458 GTAC GT^AC
AocI 2289 CCTCAGG CC^T[ACGT]AGG
ApaI 543 GGGCCC GGGCC^C
AseI 1123 ATTAAT AT^TAAT
AsnI 1123 ATTAAT AT^TAAT
AspHI 1414 GAGCTC G[AT]GC[AT]^C
AviII 1526 TGCGCA TGC^GCA
BbeI 2593 GGCGCC GGCGC^C
BbrPI 2697 CACGTG CAC^GTG
BciVI 2243 GTATCC GTATCC(6/5)
BglI 2278 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 271 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2062 GAGACC GGTCTC(1/5)
BseAI 2246 TCCGGA T^CCGGA
BsiEI 576 CGACCG CG[GA][CT]^CG
BsiHKAI 1414 GAGCTC G[AT]GC[AT]^C
BspEI 2246 TCCGGA T^CCGGA
BspHI 1802 TCATGA T^CATGA
BsrBI 2589 GAGCGG CCGCTC(-3/-3)
BsrDI 2232 CATTGC GCAATG(2/0)
BsrFI 938 GCCGGC [GA]^CCGG[CT]
BstEII 1741 GGTGACC G^GT[ACGT]ACC
BstPI 1741 GGTGACC G^GT[ACGT]ACC
Bsu36I 2289 CCTCAGG CC^T[ACGT]AGG
Cfr10I 938 GCCGGC [GA]^CCGG[CT]
DrdI 781 GACCTTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 185 CCATGG C^C[GA][CT]GG
Eco81I 2289 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1741 GGTGACC G^GT[ACGT]ACC
EcoT22I 1076 ATGCAT ATGCA^T
FseI 937 GGCCGGCC GGCCGG^CC
FspI 1526 TGCGCA TGC^GCA
HincII 610 GTCAAC GT[CT]^[GA]AC
HindII 610 GTCAAC GT[CT]^[GA]AC
KasI 2593 GGCGCC G^GCGCC
MamI 271 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 167 CAATTG C^AATTG
MroI 2246 TCCGGA T^CCGGA
MunI 167 CAATTG C^AATTG
NaeI 938 GCCGGC GCC^GGC
NarI 2593 GGCGCC GG^CGCC
NcoI 185 CCATGG C^CATGG
NgoMIV 938 GCCGGC G^CCGGC
NsiI 1076 ATGCAT ATGCA^T
NspI 340 GCATGT [GA]CATG^[CT]
PaeR7I 2190 CTCGAG C^TCGAG
PmaCI 2697 CACGTG CAC^GTG
PmlI 2697 CACGTG CAC^GTG
PshBI 1123 ATTAAT AT^TAAT
Psp1406I 2830 AACGTT AA^CGTT
RcaI 1802 TCATGA T^CATGA
RsaI 1458 GTAC GT^AC
SacI 1414 GAGCTC GAGCT^C
SbfI 2841 CCTGCAGG CCTGCA^GG
SexAI 1640 ACCTGGT A^CC[AT]GGT
SfiI 2277 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2593 GGCGCC GGC^GCC
SnaBI 69 TACGTA TAC^GTA
Sse8387I 2841 CCTGCAGG CCTGCA^GG
XcmI 89 CCACCATCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2190 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 471 AAATTT [GA]^AATT[CT]
1187 AAATTC
AlwNI 1063 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1192 CAGAGCCTG
ApoI 471 AAATTT [GA]^AATT[CT]
1187 AAATTC
AvaI 2190 CTCGAG C^[CT]CG[GA]G
2887 CTCGGG
BalI 2405 TGGCCA TGG^CCA
2861 TGGCCA
BbvCI 2597 CCTCAGC CCTCAGC(-5/-2)
853 GCTGAGG
BlpI 195 GCTGAGC GC^T[ACGT]AGC
1606 GCTGAGC
Bpu1102I 195 GCTGAGC GC^T[ACGT]AGC
1606 GCTGAGC
BsaAI 69 TACGTA [CT]AC^GT[GA]
2697 CACGTG
BsaWI 578 ACCGGA [AT]^CCGG[AT]
2246 TCCGGA
BsmBI 2088 CGTCTC CGTCTC(1/5)
972 GAGACG
BsoBI 2190 CTCGAG C^[CT]CG[GA]G
2887 CTCGGG
BspMI 2180 ACCTGC ACCTGC(4/8)
2763 GCAGGT
CelII 195 GCTGAGC GC^T[ACGT]AGC
1606 GCTGAGC
FauI 416 GCGGG CCCGC(4/6)
2445 GCGGG
HaeII 2593 GGCGCC [GA]GCGC^[CT]
2850 GGCGCT
HgaI 595 GACGC GACGC(5/10)
1167 GCGTC
MluNI 2405 TGGCCA TGG^CCA
2861 TGGCCA
MscI 2405 TGGCCA TGG^CCA
2861 TGGCCA
PpuMI 1097 AGGACCC [GA]G^G[AT]CC[CT]
2099 GGGTCCC
SmlI 1147 CTCAAG C^T[CT][GA]AG
2190 CTCGAG
TfiI 978 GATTC G^A[AT]TC
1447 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcyI AluI AlwI Asp700I
AvaII BanI BanII BbsI BbvI
BcnI BglII BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI