Restriction map for
pFN21AE6341
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BamHI
BbeI BbvCI BcgI BciVI BclI
BfaI BfrI BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
DraI DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
Hin1I HincII HindII HindIII HinfI
HpaI KasI MaeI MamI MluI
MluNI MroI MscI MseI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI SalI SapI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I Van91I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 869 GGTACC G^GTACC
AflIII 539 ACGTGT A^C[GA][CT]GT
Alw44I 952 GTGCAC G^TGCAC
AlwI 1157 GGATC GGATC(4/5)
Aor51HI 691 AGCGCT AGC^GCT
ApaI 245 GGGCCC GGGCC^C
ApaLI 952 GTGCAC G^TGCAC
Asp718I 869 GGTACC G^GTACC
AspI 985 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 538 CACGTG CAC^GTG
BbsI 844 GTCTTC GAAGAC(2/6)
BglII 1263 AGATCT A^GATCT
BpuAI 844 GTCTTC GAAGAC(2/6)
BsaAI 538 CACGTG [CT]AC^GT[GA]
BsaWI 449 ACCGGA [AT]^CCGG[AT]
BsgI 445 CTGCAC GTGCAG(16/14)
BsmAI 740 GTCTC GTCTC(1/5)
BsmBI 739 CGTCTC CGTCTC(1/5)
BssHII 1088 GCGCGC G^CGCGC
BstAPI 1004 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1263 AGATCT [GA]^GATC[CT]
CpoI 901 CGGACCG CG^G[AT]CCG
DraIII 568 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 406 CGGCCG C^GGCCG
EclXI 406 CGGCCG C^GGCCG
Eco47III 691 AGCGCT AGC^GCT
Eco52I 406 CGGCCG C^GGCCG
EcoNI 779 CCTTCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 636 GGCCGGCC GGCCGG^CC
KpnI 869 GGTACC GGTAC^C
KspI 1093 CCGCGG CCGC^GG
MfeI 609 CAATTG C^AATTG
MflI 1263 AGATCT [GA]^GATC[CT]
MslI 753 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 609 CAATTG C^AATTG
NspI 35 GCATGT [GA]CATG^[CT]
PflFI 985 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 538 CACGTG CAC^GTG
PmlI 538 CACGTG CAC^GTG
PvuII 359 CAGCTG CAG^CTG
RsrII 901 CGGACCG CG^G[AT]CCG
SacI 106 GAGCTC GAGCT^C
SacII 1093 CCGCGG CCGC^GG
SbfI 90 CCTGCAGG CCTGCA^GG
ScaI 1169 AGTACT AGT^ACT
SmaI 415 CCCGGG CCC^GGG
SmlI 697 CTCAAG C^T[CT][GA]AG
Sse8387I 90 CCTGCAGG CCTGCA^GG
Tsp509I 610 AATT ^AATT
Tth111I 985 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1263 AGATCT [GA]^GATC[CT]
XmaCI 415 CCCGGG C^CCGGG
XmaI 415 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BglI 637 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
856 GCCATCCTGGC
BseRI 73 CTCCTC GAGGAG(10/8)
76 CTCCTC
BsiEI 406 CGGCCG CG[GA][CT]^CG
717 CGACCG
DdeI 742 CTCAG C^T[ACGT]AG
1075 CTGAG
DpnII 1158 GATC ^GATC
1264 GATC
DsaI 962 CCGTGG C^C[GA][CT]GG
1093 CCGCGG
EarI 42 CTCTTC CTCTTC(1/4)
79 CTCTTC
EcoT14I 151 CCAAGG C^C[AT][AT]GG
593 CCAAGG
HgaI 391 GACGC GACGC(5/10)
420 GACGC
Ksp632I 42 CTCTTC CTCTTC(1/4)
79 CTCTTC
MaeII 539 ACGT A^CGT
950 ACGT
MboI 1158 GATC ^GATC
1264 GATC
NdeII 1158 GATC ^GATC
1264 GATC
PpuMI 95 AGGACCC [GA]G^G[AT]CC[CT]
411 GGGACCC
Sau3AI 1158 GATC ^GATC
1264 GATC
SfiI 636 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
855 GGCCATCCTGGCC
StuI 69 AGGCCT AGG^CCT
485 AGGCCT
StyI 151 CCAAGG C^C[AT][AT]GG
593 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BsaJI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NgoMIV NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TspRI TthHB8I