Restriction map for
pFN21AE6404
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiWI BsmI
BspDI BspEI BspLU11I BsrDI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NdeI NheI
NotI NspI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tsp509I Tth111I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1174 GTCTAC GT^[AC][GT]AC
AcyI 916 GGCGCC G[GA]^CG[CT]C
AflIII 1196 ACACGT A^C[GA][CT]GT
AlwI 147 GATCC GGATC(4/5)
Aor51HI 1105 AGCGCT AGC^GCT
BbeI 916 GGCGCC GGCGC^C
BbrPI 811 CACGTG CAC^GTG
BbvCI 104 CCTCAGC CCTCAGC(-5/-2)
BsaHI 916 GGCGCC G[GA]^CG[CT]C
BsaI 289 GAGACC GGTCTC(1/5)
BsiEI 140 CGGCCG CG[GA][CT]^CG
BsmBI 222 CGTCTC CGTCTC(1/5)
BspHI 1121 TCATGA T^CATGA
BssHII 1278 GCGCGC G^CGCGC
BstAPI 777 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1155 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 140 CGGCCG C^GGCCG
EclXI 140 CGGCCG C^GGCCG
Eco47III 1105 AGCGCT AGC^GCT
Eco52I 140 CGGCCG C^GGCCG
EcoT22I 129 ATGCAT ATGCA^T
Hin1I 916 GGCGCC G[GA]^CG[CT]C
KasI 916 GGCGCC G^GCGCC
NaeI 1043 GCCGGC GCC^GGC
NarI 916 GGCGCC GG^CGCC
NgoMIV 1043 GCCGGC G^CCGGC
NruI 261 TCGCGA TCG^CGA
NsiI 129 ATGCAT ATGCA^T
PmaCI 811 CACGTG CAC^GTG
PmlI 811 CACGTG CAC^GTG
PshAI 594 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 523 CTGCAG CTGCA^G
RcaI 1121 TCATGA T^CATGA
SacI 396 GAGCTC GAGCT^C
SexAI 1307 ACCAGGT A^CC[AT]GGT
SfcI 523 CTGCAG C^T[GA][CT]AG
SfiI 540 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 916 GGCGCC GGC^GCC
TfiI 462 GAATC G^A[AT]TC
TspRI 672 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BanI 502 GGTGCC G^G[CT][GA]CC
916 GGCGCC
BanII 396 GAGCTC G[GA]GC[CT]^C
991 GGGCTC
BglI 541 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
908 GCCTCCCGGGC
BsaAI 811 CACGTG [CT]AC^GT[GA]
1141 TACGTG
BsgI 135 CTGCAC GTGCAG(16/14)
898 GTGCAG
DpnII 147 GATC ^GATC
1125 GATC
DraIII 811 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1306 CACCAGGTG
EarI 67 CTCTTC CTCTTC(1/4)
82 GAAGAG
HaeII 916 GGCGCC [GA]GCGC^[CT]
1105 AGCGCT
Ksp632I 67 CTCTTC CTCTTC(1/4)
82 GAAGAG
MboI 147 GATC ^GATC
1125 GATC
MboII 82 GAAGA GAAGA(8/7)
68 TCTTC
NcoI 233 CCATGG C^CATGG
626 CCATGG
NdeII 147 GATC ^GATC
1125 GATC
PaeR7I 170 CTCGAG C^TCGAG
1063 CTCGAG
PflMI 228 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
570 CCAGAAGCTGG
PleI 63 GACTC GAGTC(4/5)
183 GAGTC
PvuII 94 CAGCTG CAG^CTG
326 CAGCTG
Sau3AI 147 GATC ^GATC
1125 GATC
SmaI 912 CCCGGG CCC^GGG
1271 CCCGGG
StuI 48 AGGCCT AGG^CCT
1115 AGGCCT
Tsp45I 1144 GTGAC ^GT[GC]AC
1324 GTCAC
Van91I 228 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
570 CCAGAAGCTGG
XcmI 79 CCAGAAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1148 CCAACTCCCACCTGG
XhoI 170 CTCGAG C^TCGAG
1063 CTCGAG
XmaCI 912 CCCGGG C^CCGGG
1271 CCCGGG
XmaI 912 CCCGGG C^CCGGG
1271 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PpuMI RsaI Sau96I ScrFI SfaNI
SmlI SspBI StyI TaqI TseI
TthHB8I