Restriction map for
pFN21AE9531
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsaHI BseAI BseRI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MseI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AlwI 191 GGATC GGATC(4/5)
ApaI 684 GGGCCC GGGCC^C
AvaI 40 CTCGAG C^[CT]CG[GA]G
AvaII 873 GGACC G^G[AT]CC
BbsI 423 GTCTTC GAAGAC(2/6)
BcnI 132 CCCGG CC^[GC]GG
BglI 430 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 165 GCTGAGC GC^T[ACGT]AGC
BpmI 637 CTCCAG CTGGAG(16/14)
Bpu1102I 165 GCTGAGC GC^T[ACGT]AGC
BpuAI 423 GTCTTC GAAGAC(2/6)
BsaWI 85 TCCGGT [AT]^CCGG[AT]
BsmBI 396 CGTCTC CGTCTC(1/5)
BsmI 201 GAATGC GAATGC(1/-1)
BsoBI 40 CTCGAG C^[CT]CG[GA]G
BsrFI 25 ACCGGC [GA]^CCGG[CT]
BstAPI 750 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 165 GCTGAGC GC^T[ACGT]AGC
CfoI 249 GCGC GCG^C
Cfr10I 25 ACCGGC [GA]^CCGG[CT]
EcoT14I 512 CCAAGG C^C[AT][AT]GG
FauI 760 CCCGC CCCGC(4/6)
HaeII 248 GGCGCT [GA]GCGC^[CT]
HgaI 666 GACGC GACGC(5/10)
HhaI 249 GCGC GCG^C
HinP1I 249 GCGC G^CGC
MaeII 725 ACGT A^CGT
MspA1I 376 CAGCTG C[AC]G^C[GT]G
NciI 132 CCCGG CC^[GC]GG
NspI 490 ACATGC [GA]CATG^[CT]
PaeR7I 40 CTCGAG C^TCGAG
PleI 144 GACTC GAGTC(4/5)
PvuII 376 CAGCTG CAG^CTG
SacI 37 GAGCTC GAGCT^C
SapI 408 GCTCTTC GCTCTTC(1/4)
SfaNI 317 GCATC GCATC(5/9)
SmlI 40 CTCGAG C^T[CT][GA]AG
StyI 512 CCAAGG C^C[AT][AT]GG
TaqI 41 TCGA T^CGA
TfiI 836 GAATC G^A[AT]TC
TthHB8I 41 TCGA T^CGA
XcmI 743 CCAAACTGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 40 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AspHI 37 GAGCTC G[AT]GC[AT]^C
406 GTGCTC
BalI 311 TGGCCA TGG^CCA
630 TGGCCA
BanI 756 GGTGCC G^G[CT][GA]CC
780 GGTGCC
BanII 37 GAGCTC G[GA]GC[CT]^C
684 GGGCCC
BsaI 7 GAGACC GGTCTC(1/5)
88 GGTCTC
BsiHKAI 37 GAGCTC G[AT]GC[AT]^C
406 GTGCTC
BsmFI 487 GGGAC GGGAC(10/14)
518 GGGAC
BsrDI 768 CATTGC GCAATG(2/0)
815 GCAATG
DraII 683 GGGGCCC [GA]G^G[ACGT]CC[CT]
684 GGGCCCC
EaeI 311 TGGCCA [CT]^GGCC[GA]
630 TGGCCA
EcoO109I 683 GGGGCCC [GA]G^G[ACGT]CC[CT]
684 GGGCCCC
HinfI 144 GACTC G^A[ACGT]TC
836 GAATC
MluNI 311 TGGCCA TGG^CCA
630 TGGCCA
MscI 311 TGGCCA TGG^CCA
630 TGGCCA
PflMI 153 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
335 CCATCGCATGG
Van91I 153 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
335 CCATCGCATGG
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwNI BbvI BmyI
BsaJI BsgI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp45I TspRI