##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MunI        
MvnI        NarI        NcoI        NdeI        NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccI          301  GTCTAC               GT^[AC][GT]AC
AciI          223  GCGG                 CCGC(-3/-1)
AfaI          183  GTAC                 GT^AC
AluI          278  AGCT                 AG^CT
AlwI          134  GGATC                GGATC(4/5)
AspI          258  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         201  GGTCC                G^G[AT]CC
BpmI          280  CTCCAG               CTGGAG(16/14)
BsrDI         168  CATTGC               GCAATG(2/0)
BsrFI         194  GCCGGC               [GA]^CCGG[CT]
BstF5I        297  GGATG                GGATG(2/0)
Cfr10I        194  GCCGGC               [GA]^CCGG[CT]
DdeI          254  CTGAG                C^T[ACGT]AG
DpnII         135  GATC                 ^GATC
EaeI          176  TGGCCG               [CT]^GGCC[GA]
EcoNI          17  CCTGCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          297  GGATG                GGATG(9/13)
HinfI         262  GAGTC                G^A[ACGT]TC
HphI          111  GGTGA                GGTGA(8/7)
MboI          135  GATC                 ^GATC
NaeI          194  GCCGGC               GCC^GGC
NdeII         135  GATC                 ^GATC
NgoMIV        194  GCCGGC               G^CCGGC
PflFI         258  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          262  GAGTC                GAGTC(4/5)
PstI           18  CTGCAG               CTGCA^G
RsaI          183  GTAC                 GT^AC
SacI          277  GAGCTC               GAGCT^C
Sau3AI        135  GATC                 ^GATC
SexAI         149  ACCTGGT              A^CC[AT]GGT
SmaI          117  CCCGGG               CCC^GGG
Tth111I       258  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         117  CCCGGG               C^CCGGG
XmaI          117  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AspHI         163  GTGCTC               G[AT]GC[AT]^C
              277  GAGCTC              
AvaI          117  CCCGGG               C^[CT]CG[GA]G
              206  CTCGGG              
BanII         114  GAGCCC               G[GA]GC[CT]^C
              277  GAGCTC              
BsiHKAI       163  GTGCTC               G[AT]GC[AT]^C
              277  GAGCTC              
BsiYI          17  CCTGCAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               75  CCAGGCCCAGG         
BslI           17  CCTGCAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               75  CCAGGCCCAGG         
BsmFI           6  GGGAC                GGGAC(10/14)
              202  GTCCC               
BsoBI         117  CCCGGG               C^[CT]CG[GA]G
              206  CTCGGG              
NlaIV          26  GGCTCC               GG[ACGT]^[ACGT]CC
              201  GGTCCC              
SfcI           18  CTGCAG               C^T[GA][CT]AG
              303  CTACAG              
TspRI         161  CAGTG                CA[GC]TG[ACGT][ACGT]^
              252  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

BbvI        BcnI        BmyI        BsaI        BsaJI       
BsmAI       Bsp1286I    BssKI       BstNI       Cac8I       
Cfr13I      EcoRII      Fnu4HI      HaeIII      HapII       
HpaII       ItaI        MnlI        MspI        MvaI        
MwoI        NciI        Sau96I      ScrFI       TseI