##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HphI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        Sau96I      SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI           15  GTAC                 GT^AC
AluI          152  AGCT                 AG^CT
BcnI          136  CCGGG                CC^[GC]GG
BsaJI         108  CCAGGG               C^C[ACGT][ACGT]GG
BseRI          70  GAGGAG               GAGGAG(10/8)
BsgI           64  CTGCAC               GTGCAG(16/14)
BssSI          67  CACGAG               CACGAG(-5/-1)
BstNI         108  CCAGG                CC^[AT]GG
BstXI         108  CCAGGGAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          27  GCAGGC               GC[ACGT]^[ACGT]GC
DpnII         170  GATC                 ^GATC
EarI          130  GAAGAG               CTCTTC(1/4)
EcoRII        108  CCAGG                ^CC[AT]GG
HaeIII          3  GGCC                 GG^CC
HapII         136  CCGG                 C^CGG
HgaI           44  GCGTC                GACGC(5/10)
HincII         46  GTCAAC               GT[CT]^[GA]AC
HindII         46  GTCAAC               GT[CT]^[GA]AC
HinfI          77  GATTC                G^A[ACGT]TC
HpaII         136  CCGG                 C^CGG
Ksp632I       130  GAAGAG               CTCTTC(1/4)
MaeIII        228  GTTAC                ^GT[ACGT]AC
MboI          170  GATC                 ^GATC
MboII         130  GAAGA                GAAGA(8/7)
MseI          143  TTAA                 T^TAA
MslI           54  CATCGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          136  CCGG                 C^CGG
MvaI          108  CCAGG                CC^[AT]GG
MwoI           66  GCACGAGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          136  CCGGG                CC^[GC]GG
NdeII         170  GATC                 ^GATC
PstI           25  CTGCAG               CTGCA^G
RsaI           15  GTAC                 GT^AC
SapI          130  GAAGAGC              GCTCTTC(1/4)
Sau3AI        170  GATC                 ^GATC
SfcI           25  CTGCAG               C^T[GA][CT]AG
SmlI           82  CTCAAG               C^T[CT][GA]AG
TaqI          168  TCGA                 T^CGA
TfiI           77  GATTC                G^A[AT]TC
Tru9I         143  TTAA                 T^TAA
TthHB8I       168  TCGA                 T^CGA
XcmI          108  CCAGGGAATTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BssKI         108  CCAGG                ^CC[ACGT]GG
              136  CCGGG               
MfeI          215  CAATTG               C^AATTG
              221  CAATTG              
MnlI           81  CCTC                 CCTC(7/6)
               70  GAGG                
MunI          215  CAATTG               C^AATTG
              221  CAATTG              
ScrFI         108  CCAGG                CC^[ACGT]GG
              136  CCGGG               
TspRI          20  CACTG                CA[GC]TG[ACGT][ACGT]^
               59  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

BbvI        Fnu4HI      ItaI        TseI        Tsp509I