Restriction map for
pFN21AE4371
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I MaeII MamI MfeI MluI
MroI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SspBI SspI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AlwNI 1094 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 804 GAGCTC G[AT]GC[AT]^C
AvaI 13 CCCGAG C^[CT]CG[GA]G
BamHI 849 GGATCC G^GATCC
BbsI 226 GTCTTC GAAGAC(2/6)
BbvCI 1160 CCTCAGC CCTCAGC(-5/-2)
BfaI 454 CTAG C^TAG
BpuAI 226 GTCTTC GAAGAC(2/6)
BsaI 986 GAGACC GGTCTC(1/5)
BsgI 19 CTGCAC GTGCAG(16/14)
BsiEI 360 CGGCCG CG[GA][CT]^CG
BsiHKAI 804 GAGCTC G[AT]GC[AT]^C
BsmAI 986 GAGAC GTCTC(1/5)
BsoBI 13 CCCGAG C^[CT]CG[GA]G
BstXI 698 CCAAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 688 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 360 CGGCCG C^GGCCG
EclXI 360 CGGCCG C^GGCCG
Eco52I 360 CGGCCG C^GGCCG
EcoNI 1032 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 57 GGCGCT [GA]GCGC^[CT]
HgaI 937 GACGC GACGC(5/10)
MaeI 454 CTAG C^TAG
MaeIII 686 GTCAC ^GT[ACGT]AC
PleI 915 GAGTC GAGTC(4/5)
SacI 804 GAGCTC GAGCT^C
SfaNI 137 GCATC GCATC(5/9)
SphI 791 GCATGC GCATG^C
TaqI 768 TCGA T^CGA
TfiI 663 GAATC G^A[AT]TC
Tsp45I 686 GTCAC ^GT[GC]AC
TthHB8I 768 TCGA T^CGA
XcmI 843 CCAGGGGGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BalI 26 TGGCCA TGG^CCA
1101 TGGCCA
BglI 49 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
353 GCCGGTTCGGC
BspMI 293 ACCTGC ACCTGC(4/8)
671 ACCTGC
BsrBI 582 CCGCTC CCGCTC(-3/-3)
741 GAGCGG
DsaI 153 CCGCGG C^C[GA][CT]GG
396 CCGCGG
HinfI 663 GAATC G^A[ACGT]TC
915 GAGTC
KspI 153 CCGCGG CCGC^GG
396 CCGCGG
MluNI 26 TGGCCA TGG^CCA
1101 TGGCCA
MscI 26 TGGCCA TGG^CCA
1101 TGGCCA
NspI 239 GCATGT [GA]CATG^[CT]
791 GCATGC
PvuII 256 CAGCTG CAG^CTG
910 CAGCTG
SacII 153 CCGCGG CCGC^GG
396 CCGCGG
SbfI 50 CCTGCAGG CCTGCA^GG
810 CCTGCAGG
Sse8387I 50 CCTGCAGG CCTGCA^GG
810 CCTGCAGG
StuI 47 AGGCCT AGG^CCT
464 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI ApaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TseI TspRI XhoII