Restriction map for
pFN21AE1539
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AlwI
AlwNI AocI ApaLI AscI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbvCI
BciVI BclI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DraII DraIII DrdI Eam1105I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
###################################
AccII 37 CGCG CG^CG
AfaI 325 GTAC GT^AC
AflII 159 CTTAAG C^TTAAG
AflIII 549 ACGTGT A^C[GA][CT]GT
Aor51HI 70 AGCGCT AGC^GCT
ApaI 866 GGGCCC GGGCC^C
AseI 529 ATTAAT AT^TAAT
AsnI 529 ATTAAT AT^TAAT
BalI 308 TGGCCA TGG^CCA
BanII 866 GGGCCC G[GA]GC[CT]^C
BbrPI 548 CACGTG CAC^GTG
BbsI 412 GTCTTC GAAGAC(2/6)
BcgI 147 CGATAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 57 CCGGG CC^[GC]GG
BfrI 159 CTTAAG C^TTAAG
BglII 395 AGATCT A^GATCT
BpuAI 412 GTCTTC GAAGAC(2/6)
BsaAI 548 CACGTG [CT]AC^GT[GA]
BsgI 734 GTGCAG GTGCAG(16/14)
BsiEI 27 CGGCCG CG[GA][CT]^CG
BspDI 145 ATCGAT AT^CGAT
BstF5I 669 GGATG GGATG(2/0)
BstUI 37 CGCG CG^CG
BstYI 395 AGATCT [GA]^GATC[CT]
ClaI 145 ATCGAT AT^CGAT
DdeI 632 CTAAG C^T[ACGT]AG
DpnII 396 GATC ^GATC
DraI 724 TTTAAA TTT^AAA
EagI 27 CGGCCG C^GGCCG
EclXI 27 CGGCCG C^GGCCG
Eco47III 70 AGCGCT AGC^GCT
Eco52I 27 CGGCCG C^GGCCG
EcoNI 689 CCTTTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 767 GAATTC G^AATTC
FauI 38 GCGGG CCCGC(4/6)
FokI 669 GGATG GGATG(9/13)
HapII 57 CCGG C^CGG
HpaII 57 CCGG C^CGG
HphI 334 GGTGA GGTGA(8/7)
KspI 36 CCGCGG CCGC^GG
MaeII 549 ACGT A^CGT
MaeIII 857 GTGAC ^GT[ACGT]AC
MboI 396 GATC ^GATC
MflI 395 AGATCT [GA]^GATC[CT]
MluNI 308 TGGCCA TGG^CCA
MscI 308 TGGCCA TGG^CCA
MslI 300 CACGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 57 CCGG C^CGG
MvnI 37 CGCG CG^CG
NciI 57 CCGGG CC^[GC]GG
NcoI 875 CCATGG C^CATGG
NdeII 396 GATC ^GATC
PmaCI 548 CACGTG CAC^GTG
PmlI 548 CACGTG CAC^GTG
PshBI 529 ATTAAT AT^TAAT
PstI 516 CTGCAG CTGCA^G
PvuII 538 CAGCTG CAG^CTG
RsaI 325 GTAC GT^AC
SacII 36 CCGCGG CCGC^GG
SapI 66 GAAGAGC GCTCTTC(1/4)
Sau3AI 396 GATC ^GATC
ScaI 324 AGTACT AGT^ACT
SmlI 159 CTTAAG C^T[CT][GA]AG
SspI 787 AATATT AAT^ATT
SwaI 723 ATTTAAAT ATTT^AAAT
Tsp45I 857 GTGAC ^GT[GC]AC
XhoII 395 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Asp700I 579 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
922 GAAGGTATTC
AvaII 190 GGACC G^G[AT]CC
555 GGACC
BfaI 268 CTAG C^TAG
676 CTAG
BmyI 297 GGGCAC G[AGT]GC[ACT]^C
866 GGGCCC
BpmI 74 CTGGAG CTGGAG(16/14)
500 CTGGAG
Bsp1286I 297 GGGCAC G[AGT]GC[ACT]^C
866 GGGCCC
BstNI 193 CCTGG CC^[AT]GG
499 CCTGG
CfoI 54 GCGC GCG^C
71 GCGC
DsaI 36 CCGCGG C^C[GA][CT]GG
875 CCATGG
EaeI 27 CGGCCG [CT]^GGCC[GA]
308 TGGCCA
EarI 399 CTCTTC CTCTTC(1/4)
66 GAAGAG
EcoRII 193 CCTGG ^CC[AT]GG
499 CCTGG
HaeII 53 AGCGCC [GA]GCGC^[CT]
70 AGCGCT
HhaI 54 GCGC GCG^C
71 GCGC
HinP1I 54 GCGC G^CGC
71 GCGC
Ksp632I 399 CTCTTC CTCTTC(1/4)
66 GAAGAG
MaeI 268 CTAG C^TAG
676 CTAG
MspA1I 36 CCGCGG C[AC]G^C[GT]G
538 CAGCTG
MvaI 193 CCTGG CC^[AT]GG
499 CCTGG
NlaIV 19 GGGGCC GG[ACGT]^[ACGT]CC
866 GGGCCC
PleI 80 GACTC GAGTC(4/5)
521 GACTC
SfcI 516 CTGCAG C^T[GA][CT]AG
762 CTACAG
TaqI 146 TCGA T^CGA
432 TCGA
TthHB8I 146 TCGA T^CGA
432 TCGA
XmnI 579 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
922 GAAGGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BsaJI BsiYI BslI BsrI BssKI
Cac8I Cfr13I EcoT14I Fnu4HI HaeIII
HinfI ItaI MboII MnlI MseI
MwoI NlaIII Sau96I ScrFI StyI
TfiI Tru9I TseI Tsp509I TspRI