Restriction map for
pFN21AE6343
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
BpmI Bpu1102I BpuAI BsaI BsaWI
BseAI BsiEI BsiHKAI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI KspI MaeI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI Tru9I Tsp509I XbaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 665 GGTACC G^GTACC
Aor51HI 97 AGCGCT AGC^GCT
Asp718I 665 GGTACC G^GTACC
AspI 391 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 308 GGTCC G^G[AT]CC
BanII 892 GAGCCC G[GA]GC[CT]^C
BbeI 61 GGCGCC GGCGC^C
BglII 686 AGATCT A^GATCT
BmyI 892 GAGCCC G[AGT]GC[ACT]^C
BsaBI 687 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 789 CTCCTC GAGGAG(10/8)
BsgI 736 CTGCAC GTGCAG(16/14)
BsiWI 338 CGTACG C^GTACG
BsmAI 478 GAGAC GTCTC(1/5)
BsmBI 478 GAGACG CGTCTC(1/5)
Bsp1286I 892 GAGCCC G[AGT]GC[ACT]^C
BsrBI 304 GAGCGG CCGCTC(-3/-3)
BsrDI 189 CATTGC GCAATG(2/0)
BssHII 50 GCGCGC G^CGCGC
BstEII 672 GGTCACC G^GT[ACGT]ACC
BstPI 672 GGTCACC G^GT[ACGT]ACC
BstYI 686 AGATCT [GA]^GATC[CT]
DraII 621 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco47III 97 AGCGCT AGC^GCT
EcoO109I 621 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 672 GGTCACC G^GT[ACGT]ACC
HincII 397 GTCAAC GT[CT]^[GA]AC
HindII 397 GTCAAC GT[CT]^[GA]AC
KasI 61 GGCGCC G^GCGCC
KpnI 665 GGTACC GGTAC^C
MamI 687 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 686 AGATCT [GA]^GATC[CT]
NarI 61 GGCGCC GG^CGCC
NspI 536 GCATGT [GA]CATG^[CT]
PaeR7I 211 CTCGAG C^TCGAG
PflFI 391 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 378 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 55 CAGCTG CAG^CTG
SapI 411 GCTCTTC GCTCTTC(1/4)
SbfI 72 CCTGCAGG CCTGCA^GG
SfoI 61 GGCGCC GGC^GCC
SmaI 870 CCCGGG CCC^GGG
Sse8387I 72 CCTGCAGG CCTGCA^GG
Tth111I 391 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 378 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 211 CTCGAG C^TCGAG
XhoII 686 AGATCT [GA]^GATC[CT]
XmaCI 870 CCCGGG C^CCGGG
XmaI 870 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 11 CGCG CG^CG
51 CGCG
AcyI 61 GGCGCC G[GA]^CG[CT]C
182 GGCGTC
AvaI 211 CTCGAG C^[CT]CG[GA]G
870 CCCGGG
BcnI 870 CCCGG CC^[GC]GG
871 CCGGG
BglI 112 GCCCGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
718 GCCATGCTGGC
BsaAI 336 CACGTA [CT]AC^GT[GA]
340 TACGTG
BsaHI 61 GGCGCC G[GA]^CG[CT]C
182 GGCGTC
BsoBI 211 CTCGAG C^[CT]CG[GA]G
870 CCCGGG
BstUI 11 CGCG CG^CG
51 CGCG
DpnII 486 GATC ^GATC
687 GATC
DraIII 379 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
644 CACCCAGTG
FauI 113 CCCGC CCCGC(4/6)
314 CCCGC
HapII 475 CCGG C^CGG
871 CCGG
Hin1I 61 GGCGCC G[GA]^CG[CT]C
182 GGCGTC
HpaII 475 CCGG C^CGG
871 CCGG
MboI 486 GATC ^GATC
687 GATC
MslI 189 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
336 CACGTACGTG
MspA1I 55 CAGCTG C[AC]G^C[GT]G
579 CAGCGG
MspI 475 CCGG C^CGG
871 CCGG
MvnI 11 CGCG CG^CG
51 CGCG
NciI 870 CCCGG CC^[GC]GG
871 CCGGG
NdeII 486 GATC ^GATC
687 GATC
PleI 391 GACTC GAGTC(4/5)
490 GACTC
PstI 73 CTGCAG CTGCA^G
157 CTGCAG
Sau3AI 486 GATC ^GATC
687 GATC
SexAI 380 ACCTGGT A^CC[AT]GGT
668 ACCTGGT
SfaNI 347 GCATC GCATC(5/9)
374 GCATC
SfcI 73 CTGCAG C^T[GA][CT]AG
157 CTGCAG
TfiI 465 GAATC G^A[AT]TC
615 GATTC
XcmI 188 CCATTGCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
371 CCAGCATCCACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwNI BanI
BbvI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI EarI EcoRII
Fnu4HI FokI HaeII HaeIII HgaI
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeII MaeIII MboII MnlI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI SmlI TaqI TseI
Tsp45I TspRI TthHB8I