Restriction map for
pFN21AE4579
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# NO CUT ENZYMES ( 104 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BcgI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1288 GACGTC GACGT^C
AccI 1858 GTAGAC GT^[AC][GT]AC
AcsI 1952 AAATTC [GA]^AATT[CT]
Alw44I 166 GTGCAC G^TGCAC
ApaI 2217 GGGCCC GGGCC^C
ApaLI 166 GTGCAC G^TGCAC
ApoI 1952 AAATTC [GA]^AATT[CT]
Asp700I 1632 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 166 GTGCAC G[AT]GC[AT]^C
BalI 135 TGGCCA TGG^CCA
BbeI 1669 GGCGCC GGCGC^C
BbrPI 1278 CACGTG CAC^GTG
BbvCI 1214 CCTCAGC CCTCAGC(-5/-2)
BciVI 770 GTATCC GTATCC(6/5)
BsaAI 1278 CACGTG [CT]AC^GT[GA]
BsiHKAI 166 GTGCAC G[AT]GC[AT]^C
BsrFI 315 GCCGGC [GA]^CCGG[CT]
BssSI 1414 CTCGTG CACGAG(-5/-1)
BstEII 1347 GGTCACC G^GT[ACGT]ACC
BstPI 1347 GGTCACC G^GT[ACGT]ACC
Cfr10I 315 GCCGGC [GA]^CCGG[CT]
EaeI 135 TGGCCA [CT]^GGCC[GA]
EcoNI 1173 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1347 GGTCACC G^GT[ACGT]ACC
FseI 314 GGCCGGCC GGCCGG^CC
KasI 1669 GGCGCC G^GCGCC
MluNI 135 TGGCCA TGG^CCA
MscI 135 TGGCCA TGG^CCA
NaeI 315 GCCGGC GCC^GGC
NarI 1669 GGCGCC GG^CGCC
NcoI 681 CCATGG C^CATGG
NgoMIV 315 GCCGGC G^CCGGC
PmaCI 1278 CACGTG CAC^GTG
PmlI 1278 CACGTG CAC^GTG
PshAI 1437 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 81 GCTCTTC GCTCTTC(1/4)
SfoI 1669 GGCGCC GGC^GCC
SmaI 483 CCCGGG CCC^GGG
SmlI 1993 CTCAAG C^T[CT][GA]AG
SphI 1076 GCATGC GCATG^C
XcmI 1049 CCATAGGAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 483 CCCGGG C^CCGGG
XmaI 483 CCCGGG C^CCGGG
XmnI 1632 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 1288 GACGTC G[GA]^CG[CT]C
1669 GGCGCC
AlwNI 397 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
726 CAGCTTCTG
AocI 607 CCTGAGG CC^T[ACGT]AGG
1957 CCTGAGG
AvaI 483 CCCGGG C^[CT]CG[GA]G
1751 CCCGAG
BamHI 1692 GGATCC G^GATCC
2187 GGATCC
BanI 461 GGCACC G^G[CT][GA]CC
1669 GGCGCC
BfaI 2158 CTAG C^TAG
2320 CTAG
BglI 300 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1643 GCCCCAGTGGC
BglII 1430 AGATCT A^GATCT
2348 AGATCT
BsaHI 1288 GACGTC G[GA]^CG[CT]C
1669 GGCGCC
BsmAI 202 GAGAC GTCTC(1/5)
1594 GTCTC
BsmI 252 GAATGC GAATGC(1/-1)
1419 GAATGC
BsoBI 483 CCCGGG C^[CT]CG[GA]G
1751 CCCGAG
Bsu36I 607 CCTGAGG CC^T[ACGT]AGG
1957 CCTGAGG
EarI 82 CTCTTC CTCTTC(1/4)
199 GAAGAG
Eco81I 607 CCTGAGG CC^T[ACGT]AGG
1957 CCTGAGG
Hin1I 1288 GACGTC G[GA]^CG[CT]C
1669 GGCGCC
Ksp632I 82 CTCTTC CTCTTC(1/4)
199 GAAGAG
MaeI 2158 CTAG C^TAG
2320 CTAG
MseI 1252 TTAA T^TAA
1477 TTAA
PflMI 1160 CCACTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1427 CCAAGATCTGG
TfiI 1494 GATTC G^A[AT]TC
2161 GAATC
Tru9I 1252 TTAA T^TAA
1477 TTAA
Tsp509I 71 AATT ^AATT
1953 AATT
Van91I 1160 CCACTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1427 CCAAGATCTGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI XhoII