Restriction map for
pFN21AE4348
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvrII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MroI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
TaqI TfiI Tsp509I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 205 CGCG CG^CG
ApaI 887 GGGCCC GGGCC^C
AviII 969 TGCGCA TGC^GCA
BalI 998 TGGCCA TGG^CCA
BbvCI 476 CCTCAGC CCTCAGC(-5/-2)
BcnI 27 CCCGG CC^[GC]GG
BpmI 815 CTGGAG CTGGAG(16/14)
BsmBI 1074 CGTCTC CGTCTC(1/5)
BspHI 735 TCATGA T^CATGA
BsrBI 1035 GAGCGG CCGCTC(-3/-3)
BsrDI 117 CATTGC GCAATG(2/0)
BsrFI 164 ACCGGC [GA]^CCGG[CT]
BstEII 699 GGTCACC G^GT[ACGT]ACC
BstPI 699 GGTCACC G^GT[ACGT]ACC
BstUI 205 CGCG CG^CG
BstYI 569 AGATCC [GA]^GATC[CT]
Cfr10I 164 ACCGGC [GA]^CCGG[CT]
DraII 887 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 749 CCATGG C^C[GA][CT]GG
EagI 906 CGGCCG C^GGCCG
EclXI 906 CGGCCG C^GGCCG
Eco52I 906 CGGCCG C^GGCCG
EcoO109I 887 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 699 GGTCACC G^GT[ACGT]ACC
FspI 969 TGCGCA TGC^GCA
MflI 569 AGATCC [GA]^GATC[CT]
MluNI 998 TGGCCA TGG^CCA
MscI 998 TGGCCA TGG^CCA
MseI 860 TTAA T^TAA
MvnI 205 CGCG CG^CG
NciI 27 CCCGG CC^[GC]GG
NcoI 749 CCATGG C^CATGG
PflMI 375 CCAAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 735 TCATGA T^CATGA
SbfI 915 CCTGCAGG CCTGCA^GG
Sse8387I 915 CCTGCAGG CCTGCA^GG
Tru9I 860 TTAA T^TAA
Van91I 375 CCAAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 569 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 325 GTAGAC GT^[AC][GT]AC
637 GTCTAC
AcyI 272 GGCGTC G[GA]^CG[CT]C
837 GGCGTC
AfaI 411 GTAC GT^AC
1026 GTAC
AlwI 570 GATCC GGATC(4/5)
800 GGATC
AlwNI 181 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
525 CAGTATCTG
AvaII 339 GGTCC G^G[AT]CC
585 GGACC
BglI 913 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
928 GCCCTGAAGGC
BsaHI 272 GGCGTC G[GA]^CG[CT]C
837 GGCGTC
BstXI 503 CCAATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
534 CCAGCAGAATGG
DpnII 570 GATC ^GATC
801 GATC
EaeI 906 CGGCCG [CT]^GGCC[GA]
998 TGGCCA
EarI 235 CTCTTC CTCTTC(1/4)
1069 CTCTTC
FauI 787 CCCGC CCCGC(4/6)
944 GCGGG
HapII 28 CCGG C^CGG
165 CCGG
HgaI 273 GCGTC GACGC(5/10)
838 GCGTC
Hin1I 272 GGCGTC G[GA]^CG[CT]C
837 GGCGTC
HinfI 103 GAGTC G^A[ACGT]TC
1063 GAGTC
HpaII 28 CCGG C^CGG
165 CCGG
Ksp632I 235 CTCTTC CTCTTC(1/4)
1069 CTCTTC
MboI 570 GATC ^GATC
801 GATC
MspI 28 CCGG C^CGG
165 CCGG
NdeII 570 GATC ^GATC
801 GATC
PleI 103 GAGTC GAGTC(4/5)
1063 GAGTC
PvuII 181 CAGCTG CAG^CTG
194 CAGCTG
RsaI 411 GTAC GT^AC
1026 GTAC
Sau3AI 570 GATC ^GATC
801 GATC
StuI 920 AGGCCT AGG^CCT
935 AGGCCT
XcmI 371 CCACCCAAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
375 CCAAGGGTTGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI Aor51HI BanI BanII
BbvI BmyI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI Eco47III EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HphI ItaI MaeIII
MboII MnlI MspA1I MvaI MwoI
NlaIII NlaIV PstI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI