Restriction map for
pFN21AE5607
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII BamHI BanI
BanII BbeI BbrPI BbvCI BclI
BcnI BfrI BglI BglII BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I
Bsp1407I BspDI BspHI BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HphI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 785 TCCGGA T^CCGGA
AflIII 459 ACATGT A^C[GA][CT]GT
AocI 614 CCTCAGG CC^T[ACGT]AGG
AvaI 1673 CTCGAG C^[CT]CG[GA]G
AvaII 47 GGTCC G^G[AT]CC
AvrII 409 CCTAGG C^CTAGG
BalI 1131 TGGCCA TGG^CCA
BbsI 1183 GAAGAC GAAGAC(2/6)
BcgI 34 GCACCCCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1324 GGATAC GTATCC(6/5)
BlnI 409 CCTAGG C^CTAGG
BpuAI 1183 GAAGAC GAAGAC(2/6)
BsaWI 785 TCCGGA [AT]^CCGG[AT]
BseAI 785 TCCGGA T^CCGGA
BsgI 32 CTGCAC GTGCAG(16/14)
BsmBI 771 GAGACG CGTCTC(1/5)
BsmFI 1210 GGGAC GGGAC(10/14)
BsoBI 1673 CTCGAG C^[CT]CG[GA]G
BspEI 785 TCCGGA T^CCGGA
BspLU11I 459 ACATGT A^CATGT
BsrBI 1231 GAGCGG CCGCTC(-3/-3)
BstAPI 22 GCAGTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 962 CCATTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 614 CCTCAGG CC^T[ACGT]AGG
DraI 243 TTTAAA TTT^AAA
EaeI 1131 TGGCCA [CT]^GGCC[GA]
Eco81I 614 CCTCAGG CC^T[ACGT]AGG
EcoRI 324 GAATTC G^AATTC
HapII 786 CCGG C^CGG
HincII 1390 GTTAAC GT[CT]^[GA]AC
HindII 1390 GTTAAC GT[CT]^[GA]AC
HpaI 1390 GTTAAC GTT^AAC
HpaII 786 CCGG C^CGG
MaeII 1716 ACGT A^CGT
MaeIII 1337 GTCAC ^GT[ACGT]AC
MluNI 1131 TGGCCA TGG^CCA
MroI 785 TCCGGA T^CCGGA
MscI 1131 TGGCCA TGG^CCA
MspA1I 1157 CAGCTG C[AC]G^C[GT]G
MspI 786 CCGG C^CGG
PaeR7I 1673 CTCGAG C^TCGAG
PleI 763 GACTC GAGTC(4/5)
PstI 146 CTGCAG CTGCA^G
PvuII 1157 CAGCTG CAG^CTG
ScaI 1196 AGTACT AGT^ACT
SspI 818 AATATT AAT^ATT
Tsp45I 1337 GTCAC ^GT[GC]AC
XhoI 1673 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
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BbvI 31 GCTGC GCAGC(8/12)
1464 GCTGC
BsiYI 37 CCCCTATCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
38 CCCTATCGGGG
BslI 37 CCCCTATCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
38 CCCTATCGGGG
BsrDI 137 GCAATG GCAATG(2/0)
871 GCAATG
BssKI 748 CCTGG ^CC[ACGT]GG
1116 CCTGG
BstNI 748 CCTGG CC^[AT]GG
1116 CCTGG
DsaI 211 CCATGG C^C[GA][CT]GG
1736 CCATGG
EcoRII 748 CCTGG ^CC[AT]GG
1116 CCTGG
FauI 1601 CCCGC CCCGC(4/6)
1233 GCGGG
MvaI 748 CCTGG CC^[AT]GG
1116 CCTGG
NcoI 211 CCATGG C^CATGG
1736 CCATGG
ScrFI 748 CCTGG CC^[ACGT]GG
1116 CCTGG
SfcI 146 CTGCAG C^T[GA][CT]AG
1027 CTACAG
SmlI 1200 CTCAAG C^T[CT][GA]AG
1673 CTCGAG
TseI 31 GCTGC G^C[AT]GC
1464 GCTGC
XcmI 557 CCAAAGTTGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
646 CCAAAACACCAGTGG
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI BfaI BpmI BsaI BsaJI
BseRI BsmAI BsrI BstF5I BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MboI MboII
MflI MnlI MseI MslI MwoI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I SfaNI StyI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I
XhoII