Restriction map for
pFN21AE1461
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tsp509I Tth111I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 1180 CGCG CG^CG
Alw44I 852 GTGCAC G^TGCAC
AlwI 306 GGATC GGATC(4/5)
ApaLI 852 GTGCAC G^TGCAC
BalI 1391 TGGCCA TGG^CCA
BbsI 1195 GTCTTC GAAGAC(2/6)
BbvCI 280 GCTGAGG CCTCAGC(-5/-2)
BcgI 141 GCACCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 418 CTAG C^TAG
BlpI 376 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 376 GCTGAGC GC^T[ACGT]AGC
BpuAI 1195 GTCTTC GAAGAC(2/6)
BsiEI 1216 CGGCCG CG[GA][CT]^CG
BsmBI 1424 GAGACG CGTCTC(1/5)
BsrDI 546 CATTGC GCAATG(2/0)
BsrI 590 CCAGT ACTGG(1/-1)
BstAPI 627 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1180 CGCG CG^CG
BstYI 306 GGATCT [GA]^GATC[CT]
CelII 376 GCTGAGC GC^T[ACGT]AGC
CfoI 189 GCGC GCG^C
DpnII 307 GATC ^GATC
DraIII 754 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1216 CGGCCG C^GGCCG
EclXI 1216 CGGCCG C^GGCCG
Eco52I 1216 CGGCCG C^GGCCG
HaeII 188 GGCGCT [GA]GCGC^[CT]
HgaI 120 GACGC GACGC(5/10)
HhaI 189 GCGC GCG^C
HinP1I 189 GCGC G^CGC
HincII 577 GTCAAC GT[CT]^[GA]AC
HindII 577 GTCAAC GT[CT]^[GA]AC
KspI 1179 CCGCGG CCGC^GG
MaeI 418 CTAG C^TAG
MaeII 866 ACGT A^CGT
MboI 307 GATC ^GATC
MflI 306 GGATCT [GA]^GATC[CT]
MluNI 1391 TGGCCA TGG^CCA
MscI 1391 TGGCCA TGG^CCA
MvnI 1180 CGCG CG^CG
NaeI 1214 GCCGGC GCC^GGC
NdeII 307 GATC ^GATC
NgoMIV 1214 GCCGGC G^CCGGC
PflMI 1008 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1412 GAGCTC GAGCT^C
SacII 1179 CCGCGG CCGC^GG
SapI 340 GAAGAGC GCTCTTC(1/4)
Sau3AI 307 GATC ^GATC
SspI 255 AATATT AAT^ATT
TfiI 470 GATTC G^A[AT]TC
Van91I 1008 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 306 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 637 CCTGAGG CC^T[ACGT]AGG
1288 CCTAAGG
AspHI 852 GTGCAC G[AT]GC[AT]^C
1412 GAGCTC
AvaII 673 GGACC G^G[AT]CC
687 GGACC
BglI 1175 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1230 GCCCCCAAGGC
BsaI 1099 GAGACC GGTCTC(1/5)
1280 GAGACC
BseRI 913 GAGGAG GAGGAG(10/8)
1090 GAGGAG
BsgI 139 CTGCAC GTGCAG(16/14)
958 CTGCAC
BsiHKAI 852 GTGCAC G[AT]GC[AT]^C
1412 GAGCTC
BsrFI 585 ACCGGC [GA]^CCGG[CT]
1214 GCCGGC
Bsu36I 637 CCTGAGG CC^T[ACGT]AGG
1288 CCTAAGG
Cfr10I 585 ACCGGC [GA]^CCGG[CT]
1214 GCCGGC
Eco81I 637 CCTGAGG CC^T[ACGT]AGG
1288 CCTAAGG
EcoT14I 1189 CCAAGG C^C[AT][AT]GG
1234 CCAAGG
MaeIII 67 GTGAC ^GT[ACGT]AC
868 GTCAC
MseI 266 TTAA T^TAA
1244 TTAA
PpuMI 672 GGGACCT [GA]G^G[AT]CC[CT]
686 GGGACCC
PvuII 15 CAGCTG CAG^CTG
1012 CAGCTG
StyI 1189 CCAAGG C^C[AT][AT]GG
1234 CCAAGG
Tru9I 266 TTAA T^TAA
1244 TTAA
Tsp45I 67 GTGAC ^GT[GC]AC
868 GTCAC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwNI ApaI AvaI
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PaeR7I PleI PstI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI TaqI TseI TspRI TthHB8I
XhoI XmaCI XmaI