Restriction map for
pFN21AE1119
##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HapII HgaI
Hin1I HpaI HpaII HphI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MflI MluI MluNI MroI
MscI MspI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 799 CTTAAG C^TTAAG
AseI 33 ATTAAT AT^TAAT
AsnI 33 ATTAAT AT^TAAT
BanI 595 GGTGCC G^G[CT][GA]CC
BbvI 24 GCAGC GCAGC(8/12)
BclI 686 TGATCA T^GATCA
BfrI 799 CTTAAG C^TTAAG
BpmI 481 CTGGAG CTGGAG(16/14)
BstF5I 256 CATCC GGATG(2/0)
DraI 525 TTTAAA TTT^AAA
DraII 732 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 732 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 619 GAATTC G^AATTC
FbaI 686 TGATCA T^GATCA
FokI 256 CATCC GGATG(9/13)
HaeIII 774 GGCC GG^CC
HincII 885 GTTGAC GT[CT]^[GA]AC
HindII 885 GTTGAC GT[CT]^[GA]AC
HindIII 559 AAGCTT A^AGCTT
MfeI 50 CAATTG C^AATTG
MslI 905 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 505 CAGCTG C[AC]G^C[GT]G
MunI 50 CAATTG C^AATTG
PflMI 58 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 732 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 33 ATTAAT AT^TAAT
PvuII 505 CAGCTG CAG^CTG
SfaNI 955 GCATC GCATC(5/9)
SphI 789 GCATGC GCATG^C
SspI 874 AATATT AAT^ATT
TaqI 130 TCGA T^CGA
TseI 24 GCAGC G^C[AT]GC
Tsp45I 903 GTCAC ^GT[GC]AC
TthHB8I 130 TCGA T^CGA
Van91I 58 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccII 18 CGCG CG^CG
20 CGCG
AciI 12 GCGG CCGC(-3/-1)
21 GCGG
AocI 247 CCTCAGG CC^T[ACGT]AGG
776 CCTCAGG
BsiYI 58 CCAGCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
159 CCATAGCCAGG
BslI 58 CCAGCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
159 CCATAGCCAGG
BstUI 18 CGCG CG^CG
20 CGCG
Bsu36I 247 CCTCAGG CC^T[ACGT]AGG
776 CCTCAGG
CfoI 19 GCGC GCG^C
55 GCGC
Eco81I 247 CCTCAGG CC^T[ACGT]AGG
776 CCTCAGG
HhaI 19 GCGC GCG^C
55 GCGC
HinP1I 19 GCGC G^CGC
55 GCGC
MvnI 18 CGCG CG^CG
20 CGCG
PleI 220 GACTC GAGTC(4/5)
320 GAGTC
SfcI 567 CTATAG C^T[GA][CT]AG
825 CTACAG
SmlI 305 CTCAAG C^T[CT][GA]AG
799 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AluI ApoI AvaII BsmFI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HinfI
ItaI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI Sau3AI Sau96I ScrFI
TfiI Tru9I Tsp509I TspRI