Restriction map for
pFN21AE5703
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AccIII AclI AflIII AgeI AhdI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BbeI BbrPI BcgI
BciVI BclI BlnI BsaAI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI MfeI MluI
MroI MslI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI RcaI RsrII SalI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1113 GACGTC GACGT^C
Acc65I 1511 GGTACC G^GTACC
AccI 868 GTAGAC GT^[AC][GT]AC
AcsI 409 AAATTT [GA]^AATT[CT]
AflII 910 CTTAAG C^TTAAG
Alw44I 1387 GTGCAC G^TGCAC
AocI 1417 CCTGAGG CC^T[ACGT]AGG
ApaLI 1387 GTGCAC G^TGCAC
ApoI 409 AAATTT [GA]^AATT[CT]
Asp718I 1511 GGTACC G^GTACC
AviII 575 TGCGCA TGC^GCA
BamHI 1487 GGATCC G^GATCC
BbsI 1576 GTCTTC GAAGAC(2/6)
BbvCI 415 GCTGAGG CCTCAGC(-5/-2)
BfrI 910 CTTAAG C^TTAAG
BglII 1472 AGATCT A^GATCT
BpuAI 1576 GTCTTC GAAGAC(2/6)
BsaBI 381 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1138 GTGCAG GTGCAG(16/14)
BsmI 753 GCATTC GAATGC(1/-1)
BsrDI 679 GCAATG GCAATG(2/0)
BsrFI 1334 GCCGGC [GA]^CCGG[CT]
BstAPI 282 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 630 CCAGGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1417 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1334 GCCGGC [GA]^CCGG[CT]
DraIII 670 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1209 GACCTTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1417 CCTGAGG CC^T[ACGT]AGG
EcoNI 624 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 575 TGCGCA TGC^GCA
HgaI 1758 GCGTC GACGC(5/10)
KpnI 1511 GGTACC GGTAC^C
KspI 1006 CCGCGG CCGC^GG
MamI 381 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 911 TTAA T^TAA
NaeI 1334 GCCGGC GCC^GGC
NgoMIV 1334 GCCGGC G^CCGGC
PflMI 1173 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 1006 CCGCGG CCGC^GG
SapI 277 GAAGAGC GCTCTTC(1/4)
SexAI 671 ACCTGGT A^CC[AT]GGT
SfcI 1317 CTACAG C^T[GA][CT]AG
SfiI 1414 GGCCCTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 647 CCCGGG CCC^GGG
SmlI 910 CTTAAG C^T[CT][GA]AG
Tru9I 911 TTAA T^TAA
Tsp509I 410 AATT ^AATT
Van91I 1173 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 647 CCCGGG C^CCGGG
XmaI 647 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 1113 GACGTC G[GA]^CG[CT]C
1757 GGCGTC
Aor51HI 42 AGCGCT AGC^GCT
1367 AGCGCT
BfaI 329 CTAG C^TAG
1570 CTAG
BglI 700 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1415 GCCCTGAGGGC
BlpI 3 GCTGAGC GC^T[ACGT]AGC
1502 GCTCAGC
Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC
1502 GCTCAGC
BsaHI 1113 GACGTC G[GA]^CG[CT]C
1757 GGCGTC
BsiEI 134 CGATCG CG[GA][CT]^CG
386 CGATCG
BsmBI 130 GAGACG CGTCTC(1/5)
1111 GAGACG
CelII 3 GCTGAGC GC^T[ACGT]AGC
1502 GCTCAGC
EarI 253 CTCTTC CTCTTC(1/4)
277 GAAGAG
Eco47III 42 AGCGCT AGC^GCT
1367 AGCGCT
HaeII 42 AGCGCT [GA]GCGC^[CT]
1367 AGCGCT
Hin1I 1113 GACGTC G[GA]^CG[CT]C
1757 GGCGTC
Ksp632I 253 CTCTTC CTCTTC(1/4)
277 GAAGAG
MaeI 329 CTAG C^TAG
1570 CTAG
MaeII 1114 ACGT A^CGT
1693 ACGT
NcoI 1478 CCATGG C^CATGG
1604 CCATGG
NspI 905 ACATGC [GA]CATG^[CT]
1391 ACATGC
PvuI 134 CGATCG CGAT^CG
386 CGATCG
PvuII 96 CAGCTG CAG^CTG
1177 CAGCTG
SacI 1011 GAGCTC GAGCT^C
1282 GAGCTC
Tsp45I 215 GTGAC ^GT[GC]AC
724 GTCAC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI TspRI TthHB8I XhoII