Restriction map for
pFN21AE2384
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI HindIII KspI
MaeI MfeI MluI MroI MspA1I
MunI MvnI NaeI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 109 GACGTC GACGT^C
AcsI 361 GAATTT [GA]^AATT[CT]
AflIII 419 ACACGT A^C[GA][CT]GT
Alw44I 240 GTGCAC G^TGCAC
ApaLI 240 GTGCAC G^TGCAC
ApoI 361 GAATTT [GA]^AATT[CT]
AvaI 667 CCCGAG C^[CT]CG[GA]G
BamHI 690 GGATCC G^GATCC
BanII 214 GAGCTC G[GA]GC[CT]^C
BbeI 28 GGCGCC GGCGC^C
BbrPI 420 CACGTG CAC^GTG
BbsI 282 GAAGAC GAAGAC(2/6)
BglI 632 GCCCCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 282 GAAGAC GAAGAC(2/6)
BsaBI 36 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 749 TCCGGT [AT]^CCGG[AT]
BseRI 211 GAGGAG GAGGAG(10/8)
BsmAI 11 GTCTC GTCTC(1/5)
BsmBI 10 CGTCTC CGTCTC(1/5)
BsmFI 66 GTCCC GGGAC(10/14)
BsoBI 667 CCCGAG C^[CT]CG[GA]G
BstEII 618 GGTGACC G^GT[ACGT]ACC
BstPI 618 GGTGACC G^GT[ACGT]ACC
BstXI 407 CCAACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 690 GGATCC [GA]^GATC[CT]
CfoI 29 GCGC GCG^C
DsaI 431 CCATGG C^C[GA][CT]GG
EcoO65I 618 GGTGACC G^GT[ACGT]ACC
EcoT14I 431 CCATGG C^C[AT][AT]GG
HaeII 28 GGCGCC [GA]GCGC^[CT]
HapII 750 CCGG C^CGG
HhaI 29 GCGC GCG^C
HinP1I 29 GCGC G^CGC
HincII 544 GTTAAC GT[CT]^[GA]AC
HindII 544 GTTAAC GT[CT]^[GA]AC
HinfI 379 GAGTC G^A[ACGT]TC
HpaI 544 GTTAAC GTT^AAC
HpaII 750 CCGG C^CGG
KasI 28 GGCGCC G^GCGCC
MamI 36 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 690 GGATCC [GA]^GATC[CT]
MslI 612 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 750 CCGG C^CGG
NarI 28 GGCGCC GG^CGCC
NcoI 431 CCATGG C^CATGG
PleI 379 GAGTC GAGTC(4/5)
PmaCI 420 CACGTG CAC^GTG
PmlI 420 CACGTG CAC^GTG
PstI 539 CTGCAG CTGCA^G
SacI 214 GAGCTC GAGCT^C
ScaI 593 AGTACT AGT^ACT
SfoI 28 GGCGCC GGC^GCC
StyI 431 CCATGG C^C[AT][AT]GG
TaqI 128 TCGA T^CGA
Tsp509I 362 AATT ^AATT
TthHB8I 128 TCGA T^CGA
XhoII 690 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 55 GGTACC G^GTACC
116 GGTACC
AcyI 28 GGCGCC G[GA]^CG[CT]C
109 GACGTC
Asp718I 55 GGTACC G^GTACC
116 GGTACC
AspHI 214 GAGCTC G[AT]GC[AT]^C
240 GTGCAC
BalI 101 TGGCCA TGG^CCA
170 TGGCCA
BbvI 712 GCAGC GCAGC(8/12)
845 GCAGC
BsaAI 243 CACGTA [CT]AC^GT[GA]
420 CACGTG
BsaHI 28 GGCGCC G[GA]^CG[CT]C
109 GACGTC
BsiHKAI 214 GAGCTC G[AT]GC[AT]^C
240 GTGCAC
BspMI 78 GCAGGT ACCTGC(4/8)
195 GCAGGT
BsrDI 807 CATTGC GCAATG(2/0)
386 GCAATG
Hin1I 28 GGCGCC G[GA]^CG[CT]C
109 GACGTC
KpnI 55 GGTACC GGTAC^C
116 GGTACC
MluNI 101 TGGCCA TGG^CCA
170 TGGCCA
MscI 101 TGGCCA TGG^CCA
170 TGGCCA
PpuMI 671 AGGACCC [GA]G^G[AT]CC[CT]
740 AGGTCCT
SapI 436 GAAGAGC GCTCTTC(1/4)
216 GCTCTTC
TseI 712 GCAGC G^C[AT]GC
845 GCAGC
TspRI 331 CACTG CA[GC]TG[ACGT][ACGT]^
399 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI Tru9I
Tsp45I XcmI