Restriction map for
pFN21AE4550
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I AspEI AspI AviII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BpmI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HpaII KasI
KspI MamI MflI MluI MroI
MspA1I MspI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PstI PvuI PvuII RcaI
RsrII SacI SacII SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 117 GGTACC G^GTACC
AclI 211 AACGTT AA^CGTT
AcyI 9 GACGCC G[GA]^CG[CT]C
AflIII 882 ACATGT A^C[GA][CT]GT
AlwI 318 GGATC GGATC(4/5)
AseI 1900 ATTAAT AT^TAAT
AsnI 1900 ATTAAT AT^TAAT
Asp718I 117 GGTACC G^GTACC
AspHI 675 GTGCTC G[AT]GC[AT]^C
AvaI 1255 CCCGAG C^[CT]CG[GA]G
AvaII 1276 GGACC G^G[AT]CC
AvrII 1266 CCTAGG C^CTAGG
BalI 488 TGGCCA TGG^CCA
BanI 117 GGTACC G^G[CT][GA]CC
BanII 1484 GAGCCC G[GA]GC[CT]^C
BbvI 1842 GCAGC GCAGC(8/12)
BlnI 1266 CCTAGG C^CTAGG
BlpI 1837 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1837 GCTAAGC GC^T[ACGT]AGC
BsaAI 410 TACGTA [CT]AC^GT[GA]
BsaHI 9 GACGCC G[GA]^CG[CT]C
BsiHKAI 675 GTGCTC G[AT]GC[AT]^C
BsoBI 1255 CCCGAG C^[CT]CG[GA]G
BspLU11I 882 ACATGT A^CATGT
BspMI 1002 GCAGGT ACCTGC(4/8)
BsrBI 16 CCGCTC CCGCTC(-3/-3)
BsrI 1160 ACTGG ACTGG(1/-1)
BssSI 1167 CTCGTG CACGAG(-5/-1)
Cac8I 1584 GCTAGC GC[ACGT]^[ACGT]GC
CelII 1837 GCTAAGC GC^T[ACGT]AGC
CfoI 37 GCGC GCG^C
Cfr13I 1276 GGACC G^G[ACGT]CC
DraI 1543 TTTAAA TTT^AAA
DraII 1275 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 488 TGGCCA [CT]^GGCC[GA]
EcoNI 1702 CCTGTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1275 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1393 GAATTC G^AATTC
HhaI 37 GCGC GCG^C
Hin1I 9 GACGCC G[GA]^CG[CT]C
HinP1I 37 GCGC G^CGC
HindIII 158 AAGCTT A^AGCTT
HpaI 1311 GTTAAC GTT^AAC
KpnI 117 GGTACC GGTAC^C
MfeI 686 CAATTG C^AATTG
MluNI 488 TGGCCA TGG^CCA
MscI 488 TGGCCA TGG^CCA
MunI 686 CAATTG C^AATTG
MwoI 30 GCCCGAAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 1584 GCTAGC G^CTAGC
PpuMI 1275 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1900 ATTAAT AT^TAAT
Psp1406I 211 AACGTT AA^CGTT
SalI 953 GTCGAC G^TCGAC
SapI 18 GCTCTTC GCTCTTC(1/4)
Sau96I 1276 GGACC G^G[ACGT]CC
SnaBI 410 TACGTA TAC^GTA
SspI 333 AATATT AAT^ATT
TseI 1842 GCAGC G^C[AT]GC
XbaI 174 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 57 GTCTAC GT^[AC][GT]AC
953 GTCGAC
AccII 38 CGCG CG^CG
44 CGCG
AlwNI 1421 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1843 CAGCTCCTG
BmyI 675 GTGCTC G[AGT]GC[ACT]^C
1484 GAGCCC
BseRI 875 GAGGAG GAGGAG(10/8)
1306 GAGGAG
Bsp1286I 675 GTGCTC G[AGT]GC[ACT]^C
1484 GAGCCC
BsrDI 419 GCAATG GCAATG(2/0)
747 GCAATG
BstUI 38 CGCG CG^CG
44 CGCG
BstXI 114 CCAGGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1733 CCAACAAGCTGG
EarI 19 CTCTTC CTCTTC(1/4)
1242 GAAGAG
EcoT14I 1266 CCTAGG C^C[AT][AT]GG
1589 CCAAGG
HgaI 9 GACGC GACGC(5/10)
42 GACGC
HincII 953 GTCGAC GT[CT]^[GA]AC
1311 GTTAAC
HindII 953 GTCGAC GT[CT]^[GA]AC
1311 GTTAAC
HphI 1627 GGTGA GGTGA(8/7)
1744 GGTGA
Ksp632I 19 CTCTTC CTCTTC(1/4)
1242 GAAGAG
MslI 339 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1824 CATGAAGATG
MvnI 38 CGCG CG^CG
44 CGCG
NspI 882 ACATGT [GA]CATG^[CT]
1376 ACATGC
PleI 1786 GACTC GAGTC(4/5)
534 GAGTC
SfaNI 1654 GATGC GCATC(5/9)
268 GCATC
StuI 68 AGGCCT AGG^CCT
839 AGGCCT
StyI 1266 CCTAGG C^C[AT][AT]GG
1589 CCAAGG
TaqI 705 TCGA T^CGA
954 TCGA
TthHB8I 705 TCGA T^CGA
954 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BfaI BpuAI BsaJI BsiYI
BslI BsmAI BssKI BstF5I BstNI
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SmlI TfiI Tru9I
Tsp45I Tsp509I TspRI