Restriction map for
pFN21AE6303
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BciVI BclI BfrI
BglII BlnI BpmI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MboI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NdeII NgoMIV
NheI NotI NruI NspI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tsp45I Tth111I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
ApaI 5 GGGCCC GGGCC^C
Asp700I 245 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 757 GGACC G^G[AT]CC
BalI 732 TGGCCA TGG^CCA
BanII 5 GGGCCC G[GA]GC[CT]^C
BbsI 967 GTCTTC GAAGAC(2/6)
BbvCI 283 CCTCAGC CCTCAGC(-5/-2)
BglI 282 GCCTCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 523 GCTGAGC GC^T[ACGT]AGC
BmyI 5 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 523 GCTGAGC GC^T[ACGT]AGC
BpuAI 967 GTCTTC GAAGAC(2/6)
BsgI 303 GTGCAG GTGCAG(16/14)
BsmAI 702 GTCTC GTCTC(1/5)
BsmFI 406 GGGAC GGGAC(10/14)
BsmI 861 GAATGC GAATGC(1/-1)
Bsp1286I 5 GGGCCC G[AGT]GC[ACT]^C
BspMI 56 ACCTGC ACCTGC(4/8)
BsrDI 603 CATTGC GCAATG(2/0)
BstBI 909 TTCGAA TT^CGAA
CelII 523 GCTGAGC GC^T[ACGT]AGC
DraII 4 GGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 732 TGGCCA [CT]^GGCC[GA]
EcoNI 463 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 4 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 454 ATGCAT ATGCA^T
FauI 37 GCGGG CCCGC(4/6)
HgaI 185 GACGC GACGC(5/10)
MaeII 413 ACGT A^CGT
MaeIII 368 GTAAC ^GT[ACGT]AC
MluNI 732 TGGCCA TGG^CCA
MscI 732 TGGCCA TGG^CCA
NdeI 764 CATATG CA^TATG
NsiI 454 ATGCAT ATGCA^T
NspV 909 TTCGAA TT^CGAA
PacI 1033 TTAATTAA TTAAT^TAA
PflMI 781 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 104 GCATC GCATC(5/9)
SfuI 909 TTCGAA TT^CGAA
SmaI 8 CCCGGG CCC^GGG
SspI 993 AATATT AAT^ATT
Van91I 781 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 8 CCCGGG C^CCGGG
XmaI 8 CCCGGG C^CCGGG
XmnI 245 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 53 GGTACC G^GTACC
598 GGTACC
AcsI 214 AAATTC [GA]^AATT[CT]
223 AAATTT
ApoI 214 AAATTC [GA]^AATT[CT]
223 AAATTT
Asp718I 53 GGTACC G^GTACC
598 GGTACC
AvaI 8 CCCGGG C^[CT]CG[GA]G
112 CTCGGG
BcgI 104 GCATCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
901 GCAGCAACTTCG
BfaI 532 CTAG C^TAG
946 CTAG
BsoBI 8 CCCGGG C^[CT]CG[GA]G
112 CTCGGG
BsrI 333 ACTGG ACTGG(1/-1)
685 ACTGG
CfoI 14 GCGC GCG^C
348 GCGC
EarI 275 CTCTTC CTCTTC(1/4)
376 CTCTTC
EcoT14I 695 CCATGG C^C[AT][AT]GG
875 CCATGG
HaeII 13 GGCGCT [GA]GCGC^[CT]
347 GGCGCT
HapII 9 CCGG C^CGG
65 CCGG
HhaI 14 GCGC GCG^C
348 GCGC
HinP1I 14 GCGC G^CGC
348 GCGC
HinfI 138 GAATC G^A[ACGT]TC
838 GAATC
HpaII 9 CCGG C^CGG
65 CCGG
HphI 208 GGTGA GGTGA(8/7)
872 TCACC
KpnI 53 GGTACC GGTAC^C
598 GGTACC
Ksp632I 275 CTCTTC CTCTTC(1/4)
376 CTCTTC
MaeI 532 CTAG C^TAG
946 CTAG
MseI 1033 TTAA T^TAA
1037 TTAA
MslI 748 CATATCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
876 CATGGGGATG
MspI 9 CCGG C^CGG
65 CCGG
NcoI 695 CCATGG C^CATGG
875 CCATGG
PvuII 163 CAGCTG CAG^CTG
521 CAGCTG
SapI 274 GCTCTTC GCTCTTC(1/4)
375 GCTCTTC
StyI 695 CCATGG C^C[AT][AT]GG
875 CCATGG
TaqI 243 TCGA T^CGA
910 TCGA
TfiI 138 GAATC G^A[AT]TC
838 GAATC
Tru9I 1033 TTAA T^TAA
1037 TTAA
TspRI 270 CACTG CA[GC]TG[ACGT][ACGT]^
292 CAGTG
TthHB8I 243 TCGA T^CGA
910 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BcnI BsaJI BsiYI BslI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DsaI EcoRII Fnu4HI FokI
HaeIII ItaI MboII MnlI MspA1I
MvaI MwoI NciI NlaIII NlaIV
RsaI Sau96I ScrFI TseI Tsp509I