Restriction map for
pFN21AE4431
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AfaI AgeI Alw44I AocI ApaI
ApaLI AscI Asp718I AspHI AviII
AvrII BamHI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BglI
BlnI BlpI BmyI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HapII HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 15 GACGTC GACGT^C
AcyI 15 GACGTC G[GA]^CG[CT]C
AflII 1306 CTTAAG C^TTAAG
AflIII 1162 ACACGT A^C[GA][CT]GT
Aor51HI 56 AGCGCT AGC^GCT
AseI 907 ATTAAT AT^TAAT
AsnI 907 ATTAAT AT^TAAT
Asp700I 1001 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 103 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 634 CTCGAG C^[CT]CG[GA]G
BalI 950 TGGCCA TGG^CCA
BanI 319 GGCACC G^G[CT][GA]CC
BfaI 565 CTAG C^TAG
BfrI 1306 CTTAAG C^TTAAG
BglII 500 AGATCT A^GATCT
BsaBI 391 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 15 GACGTC G[GA]^CG[CT]C
BsaI 166 GAGACC GGTCTC(1/5)
BsgI 1126 GTGCAG GTGCAG(16/14)
BsmI 415 GAATGC GAATGC(1/-1)
BsoBI 634 CTCGAG C^[CT]CG[GA]G
BspMI 36 GCAGGT ACCTGC(4/8)
BsrDI 1102 GCAATG GCAATG(2/0)
BssSI 84 CACGAG CACGAG(-5/-1)
BstXI 953 CCAATAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 57 GCGC GCG^C
DraI 706 TTTAAA TTT^AAA
DraII 359 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 404 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 950 TGGCCA [CT]^GGCC[GA]
Eco47III 56 AGCGCT AGC^GCT
EcoO109I 359 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 323 CCATGG C^C[AT][AT]GG
HaeII 56 AGCGCT [GA]GCGC^[CT]
HgaI 774 GACGC GACGC(5/10)
HhaI 57 GCGC GCG^C
Hin1I 15 GACGTC G[GA]^CG[CT]C
HinP1I 57 GCGC G^CGC
MaeI 565 CTAG C^TAG
MamI 391 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 950 TGGCCA TGG^CCA
MscI 950 TGGCCA TGG^CCA
MslI 33 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 323 CCATGG C^CATGG
PaeR7I 634 CTCGAG C^TCGAG
PflFI 103 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1018 GAGTC GAGTC(4/5)
PpuMI 359 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 907 ATTAAT AT^TAAT
PstI 60 CTGCAG CTGCA^G
StyI 323 CCATGG C^C[AT][AT]GG
TaqI 635 TCGA T^CGA
Tth111I 103 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 635 TCGA T^CGA
XcmI 881 CCAGTGTTCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 634 CTCGAG C^TCGAG
XmnI 1001 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcsI 703 GAATTT [GA]^AATT[CT]
970 AAATTT
AhdI 344 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
370 GACATCATGTC
ApoI 703 GAATTT [GA]^AATT[CT]
970 AAATTT
AspEI 344 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
370 GACATCATGTC
BbvCI 333 CCTCAGC CCTCAGC(-5/-2)
649 GCTGAGG
BpmI 721 CTGGAG CTGGAG(16/14)
1015 CTGGAG
BsmFI 359 GGGAC GGGAC(10/14)
1082 GGGAC
BstYI 95 AGATCC [GA]^GATC[CT]
500 AGATCT
DsaI 323 CCATGG C^C[GA][CT]GG
890 CCGTGG
Eam1105I 344 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
370 GACATCATGTC
MflI 95 AGATCC [GA]^GATC[CT]
500 AGATCT
SfaNI 759 GATGC GCATC(5/9)
307 GCATC
SfcI 60 CTGCAG C^T[GA][CT]AG
1179 CTACAG
TfiI 242 GATTC G^A[AT]TC
1302 GAATC
XhoII 95 AGATCC [GA]^GATC[CT]
500 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbsI BbvI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PflMI PvuII Sau3AI
Sau96I ScrFI SmlI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I