Restriction map for
pFN21AE1489
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII BalI BamHI
BanII BbrPI BcgI BclI BfrI
BglI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
BspEI BspHI BspLU11I BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CpoI DraI DraII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KpnI MamI MluNI MroI
MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AcsI 1117 GAATTC [GA]^AATT[CT]
AcyI 1127 GGCGCC G[GA]^CG[CT]C
AflIII 1266 ACGCGT A^C[GA][CT]GT
AocI 71 CCTCAGG CC^T[ACGT]AGG
ApoI 1117 GAATTC [GA]^AATT[CT]
Asp700I 1113 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 222 CCCGGG C^[CT]CG[GA]G
BbeI 1127 GGCGCC GGCGC^C
BbsI 1355 GTCTTC GAAGAC(2/6)
BbvCI 297 CCTCAGC CCTCAGC(-5/-2)
BciVI 231 GGATAC GTATCC(6/5)
BglII 338 AGATCT A^GATCT
BlpI 1137 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1137 GCTGAGC GC^T[ACGT]AGC
BpuAI 1355 GTCTTC GAAGAC(2/6)
BsaHI 1127 GGCGCC G[GA]^CG[CT]C
BsiEI 1155 CGATCG CG[GA][CT]^CG
BsmAI 538 GTCTC GTCTC(1/5)
BsoBI 222 CCCGGG C^[CT]CG[GA]G
Bsp1407I 203 TGTACA T^GTACA
BspDI 1153 ATCGAT AT^CGAT
BsrBI 255 GAGCGG CCGCTC(-3/-3)
BsrFI 395 ACCGGC [GA]^CCGG[CT]
BsrGI 203 TGTACA T^GTACA
BssSI 1147 CACGAG CACGAG(-5/-1)
BstAPI 437 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 338 AGATCT [GA]^GATC[CT]
Bsu36I 71 CCTCAGG CC^T[ACGT]AGG
CelII 1137 GCTGAGC GC^T[ACGT]AGC
Cfr10I 395 ACCGGC [GA]^CCGG[CT]
ClaI 1153 ATCGAT AT^CGAT
Eco81I 71 CCTCAGG CC^T[ACGT]AGG
EcoRI 1117 GAATTC G^AATTC
Hin1I 1127 GGCGCC G[GA]^CG[CT]C
HindIII 813 AAGCTT A^AGCTT
HinfI 619 GACTC G^A[ACGT]TC
KasI 1127 GGCGCC G^GCGCC
KspI 1234 CCGCGG CCGC^GG
MaeII 1094 ACGT A^CGT
MfeI 432 CAATTG C^AATTG
MflI 338 AGATCT [GA]^GATC[CT]
MluI 1266 ACGCGT A^CGCGT
MslI 627 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 432 CAATTG C^AATTG
NarI 1127 GGCGCC GG^CGCC
NspI 1205 GCATGC [GA]CATG^[CT]
PleI 619 GACTC GAGTC(4/5)
PvuI 1155 CGATCG CGAT^CG
PvuII 300 CAGCTG CAG^CTG
SacII 1234 CCGCGG CCGC^GG
SfoI 1127 GGCGCC GGC^GCC
SmaI 222 CCCGGG CCC^GGG
SphI 1205 GCATGC GCATG^C
SspBI 203 TGTACA T^GTACA
Tsp45I 905 GTGAC ^GT[GC]AC
XhoII 338 AGATCT [GA]^GATC[CT]
XmaCI 222 CCCGGG C^CCGGG
XmaI 222 CCCGGG C^CCGGG
XmnI 1113 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwNI 288 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
438 CAGCCTCTG
AvrII 99 CCTAGG C^CTAGG
1131 CCTAGG
BlnI 99 CCTAGG C^CTAGG
1131 CCTAGG
BseRI 679 CTCCTC GAGGAG(10/8)
1312 GAGGAG
BstF5I 969 CATCC GGATG(2/0)
999 CATCC
DraIII 366 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
489 CACCGTGTG
DsaI 1082 CCGTGG C^C[GA][CT]GG
1234 CCGCGG
EcoNI 94 CCTCACCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1185 CCTGCGCGAGG
FokI 969 CATCC GGATG(9/13)
999 CATCC
HgaI 1071 GACGC GACGC(5/10)
1265 GACGC
MaeIII 548 GTAAC ^GT[ACGT]AC
905 GTGAC
MseI 605 TTAA T^TAA
694 TTAA
MspA1I 300 CAGCTG C[AC]G^C[GT]G
1234 CCGCGG
Tru9I 605 TTAA T^TAA
694 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
ApaLI AspHI AvaII BanI BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeI
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfcI StyI TaqI TseI
Tsp509I TspRI TthHB8I