Restriction map for
pFN21AE5227
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I TspRI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwI 416 GGATC GGATC(4/5)
AocI 502 CCTAAGG CC^T[ACGT]AGG
AvaI 408 CTCGGG C^[CT]CG[GA]G
BanI 423 GGCACC G^G[CT][GA]CC
BfaI 79 CTAG C^TAG
BsaI 295 GGTCTC GGTCTC(1/5)
BseRI 61 GAGGAG GAGGAG(10/8)
BsgI 345 CTGCAC GTGCAG(16/14)
BsiWI 488 CGTACG C^GTACG
BsoBI 408 CTCGGG C^[CT]CG[GA]G
BspMI 122 ACCTGC ACCTGC(4/8)
BstF5I 147 GGATG GGATG(2/0)
Bsu36I 502 CCTAAGG CC^T[ACGT]AGG
Cac8I 377 GCCAGC GC[ACGT]^[ACGT]GC
EaeI 311 TGGCCG [CT]^GGCC[GA]
Eco81I 502 CCTAAGG CC^T[ACGT]AGG
EcoT14I 237 CCTTGG C^C[AT][AT]GG
FokI 147 GGATG GGATG(9/13)
HinfI 75 GATTC G^A[ACGT]TC
MaeI 79 CTAG C^TAG
MaeIII 37 GTAAC ^GT[ACGT]AC
MseI 283 TTAA T^TAA
MslI 99 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 546 GCTGGAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 95 CATG CATG^
PflMI 303 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 214 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 553 CTGCAG CTGCA^G
ScaI 560 AGTACT AGT^ACT
SfaNI 498 GATGC GCATC(5/9)
SfcI 553 CTGCAG C^T[GA][CT]AG
StyI 237 CCTTGG C^C[AT][AT]GG
TaqI 320 TCGA T^CGA
TfiI 75 GATTC G^A[AT]TC
Tru9I 283 TTAA T^TAA
Tsp509I 117 AATT ^AATT
TthHB8I 320 TCGA T^CGA
Van91I 303 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 4 GCGG CCGC(-3/-1)
508 GCGG
AlwNI 304 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
429 CAGAGGCTG
ApaI 196 GGGCCC GGGCC^C
539 GGGCCC
BsmAI 296 GTCTC GTCTC(1/5)
523 GTCTC
BsmFI 368 GGGAC GGGAC(10/14)
452 GGGAC
BstXI 50 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
438 CCAGGACTTTGG
CfoI 340 GCGC GCG^C
387 GCGC
DdeI 358 CTCAG C^T[ACGT]AG
503 CTAAG
DpnII 27 GATC ^GATC
417 GATC
HhaI 340 GCGC GCG^C
387 GCGC
HinP1I 340 GCGC G^CGC
387 GCGC
MaeII 349 ACGT A^CGT
487 ACGT
MboI 27 GATC ^GATC
417 GATC
NdeII 27 GATC ^GATC
417 GATC
PpuMI 368 GGGACCC [GA]G^G[AT]CC[CT]
452 GGGACCC
Sau3AI 27 GATC ^GATC
417 GATC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cfr13I
DraII EcoO109I EcoRII Fnu4HI HaeIII
HapII HpaII ItaI MboII MnlI
MspI MvaI NciI NlaIV RsaI
Sau96I ScrFI TseI