Restriction map for
pFN21AB6266
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# NO CUT ENZYMES ( 95 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BpuAI BsaBI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstBI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I EarI Eco47III Eco81I
EcoRV EcoT22I FbaI FseI FspI
HindIII KasI KpnI Ksp632I MamI
MfeI MluI MroI MunI NarI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SexAI SfiI
SfoI SfuI SgrAI SnaBI SspI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 1710 GACGTC GACGT^C
Alw44I 266 GTGCAC G^TGCAC
ApaLI 266 GTGCAC G^TGCAC
BanII 1554 GAGCCC G[GA]GC[CT]^C
BglII 1976 AGATCT A^GATCT
BsaI 1018 GGTCTC GGTCTC(1/5)
BsaWI 80 ACCGGA [AT]^CCGG[AT]
BsiEI 1220 CGGCCG CG[GA][CT]^CG
BsmI 357 GAATGC GAATGC(1/-1)
Bsp1407I 1496 TGTACA T^GTACA
BspLU11I 1187 ACATGT A^CATGT
BsrDI 2036 GCAATG GCAATG(2/0)
BsrGI 1496 TGTACA T^GTACA
BssSI 1485 CACGAG CACGAG(-5/-1)
BstEII 339 GGTGACC G^GT[ACGT]ACC
BstPI 339 GGTGACC G^GT[ACGT]ACC
DraIII 178 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1909 GACAACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1220 CGGCCG C^GGCCG
EclXI 1220 CGGCCG C^GGCCG
Eco52I 1220 CGGCCG C^GGCCG
EcoNI 1932 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 339 GGTGACC G^GT[ACGT]ACC
EcoRI 94 GAATTC G^AATTC
HincII 1051 GTTAAC GT[CT]^[GA]AC
HindII 1051 GTTAAC GT[CT]^[GA]AC
HpaI 1051 GTTAAC GTT^AAC
KspI 1217 CCGCGG CCGC^GG
NdeI 203 CATATG CA^TATG
NotI 1219 GCGGCCGC GC^GGCCGC
PflMI 1886 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 1217 CCGCGG CCGC^GG
SbfI 105 CCTGCAGG CCTGCA^GG
SmaI 803 CCCGGG CCC^GGG
SmlI 1071 CTTGAG C^T[CT][GA]AG
SpeI 1821 ACTAGT A^CTAGT
SphI 1419 GCATGC GCATG^C
Sse8387I 105 CCTGCAGG CCTGCA^GG
SspBI 1496 TGTACA T^GTACA
StuI 1005 AGGCCT AGG^CCT
Van91I 1886 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 803 CCCGGG C^CCGGG
XmaI 803 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwI 1264 GATCC GGATC(4/5)
1803 GGATC
BalI 405 TGGCCA TGG^CCA
1739 TGGCCA
BbrPI 1492 CACGTG CAC^GTG
2053 CACGTG
BlpI 388 GCTCAGC GC^T[ACGT]AGC
2226 GCTTAGC
Bpu1102I 388 GCTCAGC GC^T[ACGT]AGC
2226 GCTTAGC
BsaAI 1492 CACGTG [CT]AC^GT[GA]
2053 CACGTG
BseRI 873 CTCCTC GAGGAG(10/8)
2161 CTCCTC
BsrFI 14 GCCGGC [GA]^CCGG[CT]
484 GCCGGC
BstAPI 375 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
784 GCAAGCCCTGC
BstYI 1803 GGATCT [GA]^GATC[CT]
1976 AGATCT
CelII 388 GCTCAGC GC^T[ACGT]AGC
2226 GCTTAGC
Cfr10I 14 GCCGGC [GA]^CCGG[CT]
484 GCCGGC
MboII 772 TCTTC GAAGA(8/7)
1979 TCTTC
MflI 1803 GGATCT [GA]^GATC[CT]
1976 AGATCT
MluNI 405 TGGCCA TGG^CCA
1739 TGGCCA
MscI 405 TGGCCA TGG^CCA
1739 TGGCCA
NaeI 14 GCCGGC GCC^GGC
484 GCCGGC
NcoI 344 CCATGG C^CATGG
1250 CCATGG
NgoMIV 14 GCCGGC G^CCGGC
484 GCCGGC
PmaCI 1492 CACGTG CAC^GTG
2053 CACGTG
PmlI 1492 CACGTG CAC^GTG
2053 CACGTG
ScaI 1654 AGTACT AGT^ACT
2143 AGTACT
TaqI 818 TCGA T^CGA
1571 TCGA
TthHB8I 818 TCGA T^CGA
1571 TCGA
XcmI 1056 CCAAGCGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1664 CCATTTGGGACCTGG
XhoII 1803 GGATCT [GA]^GATC[CT]
1976 AGATCT
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwNI ApoI AspHI
AvaI AvaII BanI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI