Restriction map for
pFN21AE3946
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrFI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 350 GTCGAC GT^[AC][GT]AC
Aor51HI 65 AGCGCT AGC^GCT
ApaI 742 GGGCCC GGGCC^C
BamHI 243 GGATCC G^GATCC
BbeI 328 GGCGCC GGCGC^C
BbrPI 208 CACGTG CAC^GTG
BclI 257 TGATCA T^GATCA
BlpI 68 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 68 GCTCAGC GC^T[ACGT]AGC
BsaAI 208 CACGTG [CT]AC^GT[GA]
BsmFI 165 GTCCC GGGAC(10/14)
Bsp1407I 764 TGTACA T^GTACA
BspMI 458 ACCTGC ACCTGC(4/8)
BsrBI 88 GAGCGG CCGCTC(-3/-3)
BsrDI 494 GCAATG GCAATG(2/0)
BsrGI 764 TGTACA T^GTACA
BssSI 418 CACGAG CACGAG(-5/-1)
BstXI 203 CCAGCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 243 GGATCC [GA]^GATC[CT]
CelII 68 GCTCAGC GC^T[ACGT]AGC
Eco47III 65 AGCGCT AGC^GCT
EcoT14I 248 CCAAGG C^C[AT][AT]GG
FbaI 257 TGATCA T^GATCA
HgaI 395 GCGTC GACGC(5/10)
HincII 350 GTCGAC GT[CT]^[GA]AC
HindII 350 GTCGAC GT[CT]^[GA]AC
KasI 328 GGCGCC G^GCGCC
MflI 243 GGATCC [GA]^GATC[CT]
NarI 328 GGCGCC GG^CGCC
PmaCI 208 CACGTG CAC^GTG
PmlI 208 CACGTG CAC^GTG
PpuMI 163 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 727 GAGCTC GAGCT^C
SalI 350 GTCGAC G^TCGAC
SfoI 328 GGCGCC GGC^GCC
SmlI 289 CTCAAG C^T[CT][GA]AG
SspBI 764 TGTACA T^GTACA
StyI 248 CCAAGG C^C[AT][AT]GG
TfiI 123 GATTC G^A[AT]TC
XhoII 243 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 328 GGCGCC G[GA]^CG[CT]C
394 GGCGTC
AspHI 727 GAGCTC G[AT]GC[AT]^C
864 GAGCAC
AvaII 164 GGTCC G^G[AT]CC
469 GGTCC
BbsI 705 GAAGAC GAAGAC(2/6)
856 GTCTTC
BpuAI 705 GAAGAC GAAGAC(2/6)
856 GTCTTC
BsaHI 328 GGCGCC G[GA]^CG[CT]C
394 GGCGTC
BsaI 272 GAGACC GGTCTC(1/5)
594 GGTCTC
BseRI 573 CTCCTC GAGGAG(10/8)
55 GAGGAG
BsiHKAI 727 GAGCTC G[AT]GC[AT]^C
864 GAGCAC
BsmAI 272 GAGAC GTCTC(1/5)
595 GTCTC
BsrI 369 ACTGG ACTGG(1/-1)
278 CCAGT
BssHII 99 GCGCGC G^CGCGC
101 GCGCGC
DraII 163 GGGTCCC [GA]G^G[ACGT]CC[CT]
742 GGGCCCC
DraIII 486 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
691 CACCATGTG
EcoO109I 163 GGGTCCC [GA]G^G[ACGT]CC[CT]
742 GGGCCCC
Hin1I 328 GGCGCC G[GA]^CG[CT]C
394 GGCGTC
MslI 208 CACGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
432 CACACCTGTG
PleI 236 GAGTC GAGTC(4/5)
854 GAGTC
SfaNI 181 GATGC GCATC(5/9)
423 GCATC
SmaI 50 CCCGGG CCC^GGG
324 CCCGGG
TaqI 239 TCGA T^CGA
351 TCGA
Tsp45I 199 GTGAC ^GT[GC]AC
397 GTCAC
TthHB8I 239 TCGA T^CGA
351 TCGA
XcmI 456 CCACCTGCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
800 CCACAGCAGACGTGG
XmaCI 50 CCCGGG C^CCGGG
324 CCCGGG
XmaI 50 CCCGGG C^CCGGG
324 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TseI TspRI