Restriction map for
pFN21AE4541
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII AccI AclI AcyI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BlnI BpmI BsaHI BsaI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI DraI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I KasI KspI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1773 GGTACC G^GTACC
AccIII 1409 TCCGGA T^CCGGA
AcsI 1029 AAATTC [GA]^AATT[CT]
AfaI 1774 GTAC GT^AC
AocI 297 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1642 AGCGCT AGC^GCT
ApaI 1273 GGGCCC GGGCC^C
ApoI 1029 AAATTC [GA]^AATT[CT]
Asp718I 1773 GGTACC G^GTACC
AvaI 385 CTCGGG C^[CT]CG[GA]G
BamHI 1664 GGATCC G^GATCC
BbsI 1267 GAAGAC GAAGAC(2/6)
BpuAI 1267 GAAGAC GAAGAC(2/6)
BsaAI 65 TACGTA [CT]AC^GT[GA]
BsaWI 1409 TCCGGA [AT]^CCGG[AT]
BseAI 1409 TCCGGA T^CCGGA
BseRI 664 GAGGAG GAGGAG(10/8)
BsoBI 385 CTCGGG C^[CT]CG[GA]G
BspEI 1409 TCCGGA T^CCGGA
BsrFI 412 ACCGGC [GA]^CCGG[CT]
BstAPI 27 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 297 CCTGAGG CC^T[ACGT]AGG
Cfr10I 412 ACCGGC [GA]^CCGG[CT]
CpoI 1067 CGGACCG CG^G[AT]CCG
DraIII 655 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1456 GACTTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1488 GAAGAG CTCTTC(1/4)
Eco47III 1642 AGCGCT AGC^GCT
Eco81I 297 CCTGAGG CC^T[ACGT]AGG
HgaI 874 GACGC GACGC(5/10)
HincII 1765 GTTAAC GT[CT]^[GA]AC
HindII 1765 GTTAAC GT[CT]^[GA]AC
HindIII 344 AAGCTT A^AGCTT
HpaI 1765 GTTAAC GTT^AAC
KpnI 1773 GGTACC GGTAC^C
Ksp632I 1488 GAAGAG CTCTTC(1/4)
MroI 1409 TCCGGA T^CCGGA
MseI 1766 TTAA T^TAA
NcoI 497 CCATGG C^CATGG
PpuMI 182 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1793 CAGCTG CAG^CTG
RsaI 1774 GTAC GT^AC
RsrII 1067 CGGACCG CG^G[AT]CCG
SapI 1488 GAAGAGC GCTCTTC(1/4)
SexAI 1502 ACCAGGT A^CC[AT]GGT
SnaBI 65 TACGTA TAC^GTA
Tru9I 1766 TTAA T^TAA
Tsp509I 1030 AATT ^AATT
XbaI 543 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 28 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
967 CAGGCACTG
AspHI 214 GTGCTC G[AT]GC[AT]^C
1060 GAGCAC
BlpI 799 GCTGAGC GC^T[ACGT]AGC
1795 GCTGAGC
Bpu1102I 799 GCTGAGC GC^T[ACGT]AGC
1795 GCTGAGC
BsaBI 594 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1578 GATCAACATC
BsgI 1534 CTGCAC GTGCAG(16/14)
1721 GTGCAG
BsiHKAI 214 GTGCTC G[AT]GC[AT]^C
1060 GAGCAC
BssSI 1537 CACGAG CACGAG(-5/-1)
1646 CTCGTG
BstEII 1506 GGTGACC G^GT[ACGT]ACC
1527 GGTGACC
BstPI 1506 GGTGACC G^GT[ACGT]ACC
1527 GGTGACC
BstXI 638 CCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
788 CCAAAAACATGG
CelII 799 GCTGAGC GC^T[ACGT]AGC
1795 GCTGAGC
DsaI 497 CCATGG C^C[GA][CT]GG
1673 CCGTGG
EcoO65I 1506 GGTGACC G^GT[ACGT]ACC
1527 GGTGACC
HaeII 127 AGCGCC [GA]GCGC^[CT]
1642 AGCGCT
MamI 594 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1578 GATCAACATC
NspI 633 GCATGC [GA]CATG^[CT]
905 GCATGC
PflMI 1342 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1777 CCAAGAAGTGG
PleI 443 GAGTC GAGTC(4/5)
763 GAGTC
SphI 633 GCATGC GCATG^C
905 GCATGC
TfiI 192 GAATC G^A[AT]TC
1197 GATTC
Van91I 1342 CCAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1777 CCAAGAAGTGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BalI BanI BanII BbvI BcgI
BcnI BfaI BglII BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII