Restriction map for
pFN21AE1515
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# NO CUT ENZYMES ( 143 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbeI
BbrPI BbvI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstF5I BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HaeII
HgaI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
Tru9I TseI Tth111I TthHB8I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 568 GACGTC GACGT^C
AccII 123 CGCG CG^CG
AcsI 166 GAATTT [GA]^AATT[CT]
AcyI 568 GACGTC G[GA]^CG[CT]C
AfaI 130 GTAC GT^AC
AhdI 103 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 473 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 352 CCTGAGG CC^T[ACGT]AGG
ApoI 166 GAATTT [GA]^AATT[CT]
AspEI 103 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 761 CCCGGG C^[CT]CG[GA]G
BalI 750 TGGCCA TGG^CCA
BanI 718 GGCACC G^G[CT][GA]CC
BbsI 10 GAAGAC GAAGAC(2/6)
BbvCI 557 CCTCAGC CCTCAGC(-5/-2)
BfaI 700 CTAG C^TAG
BpmI 226 CTGGAG CTGGAG(16/14)
BpuAI 10 GAAGAC GAAGAC(2/6)
BsaHI 568 GACGTC G[GA]^CG[CT]C
BsaI 211 GAGACC GGTCTC(1/5)
BsoBI 761 CCCGGG C^[CT]CG[GA]G
Bsp1407I 129 TGTACA T^GTACA
BsrBI 610 CCGCTC CCGCTC(-3/-3)
BsrGI 129 TGTACA T^GTACA
BstEII 357 GGTGACC G^GT[ACGT]ACC
BstPI 357 GGTGACC G^GT[ACGT]ACC
BstUI 123 CGCG CG^CG
BstYI 195 GGATCT [GA]^GATC[CT]
Bsu36I 352 CCTGAGG CC^T[ACGT]AGG
CfoI 3 GCGC GCG^C
Eam1105I 103 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 499 CTCTTC CTCTTC(1/4)
Eco81I 352 CCTGAGG CC^T[ACGT]AGG
EcoO65I 357 GGTGACC G^GT[ACGT]ACC
FauI 266 GCGGG CCCGC(4/6)
HapII 762 CCGG C^CGG
HhaI 3 GCGC GCG^C
Hin1I 568 GACGTC G[GA]^CG[CT]C
HinP1I 3 GCGC G^CGC
HincII 111 GTCAAC GT[CT]^[GA]AC
HindII 111 GTCAAC GT[CT]^[GA]AC
HinfI 72 GAGTC G^A[ACGT]TC
HpaII 762 CCGG C^CGG
HphI 357 GGTGA GGTGA(8/7)
Ksp632I 499 CTCTTC CTCTTC(1/4)
MaeI 700 CTAG C^TAG
MflI 195 GGATCT [GA]^GATC[CT]
MluNI 750 TGGCCA TGG^CCA
MscI 750 TGGCCA TGG^CCA
MspI 762 CCGG C^CGG
MvnI 123 CGCG CG^CG
PleI 72 GAGTC GAGTC(4/5)
PstI 579 CTGCAG CTGCA^G
RsaI 130 GTAC GT^AC
SfcI 579 CTGCAG C^T[GA][CT]AG
SmaI 761 CCCGGG CCC^GGG
SspBI 129 TGTACA T^GTACA
Tsp509I 167 AATT ^AATT
XcmI 371 CCAAGGAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 195 GGATCT [GA]^GATC[CT]
XmaCI 761 CCCGGG C^CCGGG
XmaI 761 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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ApaI 764 GGGCCC GGGCC^C
772 GGGCCC
AvaII 102 GGACC G^G[AT]CC
603 GGACC
BcnI 761 CCCGG CC^[GC]GG
762 CCGGG
BglI 91 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
698 GCCTAGTGGGC
BsrDI 279 CATTGC GCAATG(2/0)
461 GCAATG
DraII 771 GGGGCCC [GA]G^G[ACGT]CC[CT]
772 GGGCCCC
EaeI 237 TGGCCG [CT]^GGCC[GA]
750 TGGCCA
EcoO109I 771 GGGGCCC [GA]G^G[ACGT]CC[CT]
772 GGGCCCC
EcoT14I 56 CCTTGG C^C[AT][AT]GG
371 CCAAGG
MaeII 446 ACGT A^CGT
569 ACGT
MslI 452 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
708 CATCATCGTG
NciI 761 CCCGG CC^[GC]GG
762 CCGGG
SmlI 42 CTTGAG C^T[CT][GA]AG
616 CTCAAG
StyI 56 CCTTGG C^C[AT][AT]GG
371 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI AspHI BanII
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI Tsp45I TspRI