Restriction map for
pFN21AE0897
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeIII HindIII
HinfI HpaI KpnI KspI MaeII
MaeIII MamI MfeI MluI MluNI
MscI MspA1I MunI MvnI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfcI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Tth111I
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 161 TCCGGA T^CCGGA
AcsI 229 AAATTT [GA]^AATT[CT]
AflIII 554 ACATGT A^C[GA][CT]GT
AlwI 177 GGATC GGATC(4/5)
AlwNI 361 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 229 AAATTT [GA]^AATT[CT]
BanI 695 GGCGCC G^G[CT][GA]CC
BbeI 695 GGCGCC GGCGC^C
BcnI 638 CCCGG CC^[GC]GG
BsaI 525 GGTCTC GGTCTC(1/5)
BsaWI 161 TCCGGA [AT]^CCGG[AT]
BseAI 161 TCCGGA T^CCGGA
BsgI 331 GTGCAG GTGCAG(16/14)
BsiYI 686 CCAGTGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 686 CCAGTGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspEI 161 TCCGGA T^CCGGA
BspLU11I 554 ACATGT A^CATGT
BstYI 177 GGATCT [GA]^GATC[CT]
DsaI 5 CCATGG C^C[GA][CT]GG
EarI 751 CTCTTC CTCTTC(1/4)
EcoT14I 5 CCATGG C^C[AT][AT]GG
HaeII 695 GGCGCC [GA]GCGC^[CT]
HgaI 423 GCGTC GACGC(5/10)
HphI 27 GGTGA GGTGA(8/7)
KasI 695 GGCGCC G^GCGCC
Ksp632I 751 CTCTTC CTCTTC(1/4)
MflI 177 GGATCT [GA]^GATC[CT]
MroI 161 TCCGGA T^CCGGA
MseI 233 TTAA T^TAA
MslI 537 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 695 GGCGCC GG^CGCC
NciI 638 CCCGG CC^[GC]GG
NcoI 5 CCATGG C^CATGG
NdeI 679 CATATG CA^TATG
NspI 554 ACATGT [GA]CATG^[CT]
ScaI 280 AGTACT AGT^ACT
SfoI 695 GGCGCC GGC^GCC
StyI 5 CCATGG C^C[AT][AT]GG
TaqI 128 TCGA T^CGA
Tru9I 233 TTAA T^TAA
TthHB8I 128 TCGA T^CGA
XbaI 180 TCTAGA T^CTAGA
XhoII 177 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 422 GGCGTC G[GA]^CG[CT]C
695 GGCGCC
AfaI 40 GTAC GT^AC
281 GTAC
BanII 88 GAGCTC G[GA]GC[CT]^C
436 GAGCTC
BfaI 181 CTAG C^TAG
224 CTAG
BsaHI 422 GGCGTC G[GA]^CG[CT]C
695 GGCGCC
BseRI 91 CTCCTC GAGGAG(10/8)
466 GAGGAG
BsmAI 374 GAGAC GTCTC(1/5)
526 GTCTC
BstNI 63 CCAGG CC^[AT]GG
96 CCAGG
Cac8I 333 GCAGGC GC[ACGT]^[ACGT]GC
474 GCAGGC
CfoI 564 GCGC GCG^C
696 GCGC
EcoRII 63 CCAGG ^CC[AT]GG
96 CCAGG
HapII 162 CCGG C^CGG
639 CCGG
HhaI 564 GCGC GCG^C
696 GCGC
Hin1I 422 GGCGTC G[GA]^CG[CT]C
695 GGCGCC
HinP1I 564 GCGC G^CGC
696 GCGC
HincII 52 GTCAAC GT[CT]^[GA]AC
318 GTCAAC
HindII 52 GTCAAC GT[CT]^[GA]AC
318 GTCAAC
HpaII 162 CCGG C^CGG
639 CCGG
MaeI 181 CTAG C^TAG
224 CTAG
MspI 162 CCGG C^CGG
639 CCGG
MvaI 63 CCAGG CC^[AT]GG
96 CCAGG
RsaI 40 GTAC GT^AC
281 GTAC
SacI 88 GAGCTC GAGCT^C
436 GAGCTC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI AspHI AvaII BbvI BmyI
BpmI BsaJI BsiHKAI BsmFI Bsp1286I
BsrI BssKI BstF5I Cfr13I DdeI
DpnII DraII EcoO109I Fnu4HI FokI
ItaI MboI MboII MnlI MwoI
NdeII NlaIII NlaIV PpuMI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp509I
TspRI