Restriction map for
pFN21AE6709
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BbrPI BbsI BcgI BciVI
BclI BfrI BlnI BpmI BpuAI
BsaAI BsaWI BseAI BseRI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII Bst1107I BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco52I EcoO65I EcoRV FbaI FseI
FspI KpnI Ksp632I KspI MluI
MluNI MroI MscI MvnI NaeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 130 GGCGCC G[GA]^CG[CT]C
AflIII 1533 ACACGT A^C[GA][CT]GT
AocI 1773 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1982 AGCGCT AGC^GCT
AspHI 328 GAGCTC G[AT]GC[AT]^C
BbeI 130 GGCGCC GGCGC^C
BbvCI 1567 GCTGAGG CCTCAGC(-5/-2)
BglII 867 AGATCT A^GATCT
BlpI 855 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 855 GCTCAGC GC^T[ACGT]AGC
BsaBI 469 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 130 GGCGCC G[GA]^CG[CT]C
BsgI 1097 GTGCAG GTGCAG(16/14)
BsiEI 1919 CGGTCG CG[GA][CT]^CG
BsiHKAI 328 GAGCTC G[AT]GC[AT]^C
BspHI 1269 TCATGA T^CATGA
BsrBI 1916 GAGCGG CCGCTC(-3/-3)
BssSI 1873 CTCGTG CACGAG(-5/-1)
BstAPI 1503 GCATTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2113 TTCGAA TT^CGAA
BstXI 2027 CCATGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1773 CCTGAGG CC^T[ACGT]AGG
CelII 855 GCTCAGC GC^T[ACGT]AGC
DraIII 1870 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 353 TGGCCG [CT]^GGCC[GA]
Eco47III 1982 AGCGCT AGC^GCT
Eco81I 1773 CCTGAGG CC^T[ACGT]AGG
EcoNI 1219 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1779 GAATTC G^AATTC
HgaI 8 GCGTC GACGC(5/10)
Hin1I 130 GGCGCC G[GA]^CG[CT]C
HincII 1618 GTTAAC GT[CT]^[GA]AC
HindII 1618 GTTAAC GT[CT]^[GA]AC
HindIII 419 AAGCTT A^AGCTT
HpaI 1618 GTTAAC GTT^AAC
KasI 130 GGCGCC G^GCGCC
MamI 469 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 832 CAATTG C^AATTG
MunI 832 CAATTG C^AATTG
NarI 130 GGCGCC GG^CGCC
NdeI 1832 CATATG CA^TATG
NspV 2113 TTCGAA TT^CGAA
PpuMI 1067 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1706 CTGCAG CTGCA^G
RcaI 1269 TCATGA T^CATGA
SacI 328 GAGCTC GAGCT^C
ScaI 1592 AGTACT AGT^ACT
SexAI 1546 ACCAGGT A^CC[AT]GGT
SfoI 130 GGCGCC GGC^GCC
SfuI 2113 TTCGAA TT^CGAA
SphI 1153 GCATGC GCATG^C
StuI 1164 AGGCCT AGG^CCT
Tsp45I 1537 GTCAC ^GT[GC]AC
XbaI 588 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwNI 941 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1651 CAGCCACTG
ApaI 95 GGGCCC GGGCC^C
301 GGGCCC
Asp700I 223 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
706 GAACACATTC
BamHI 89 GGATCC G^GATCC
1956 GGATCC
BglI 59 GCCGGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
355 GCCGACTTGGC
BsaI 119 GGTCTC GGTCTC(1/5)
757 GGTCTC
BsmI 397 GCATTC GAATGC(1/-1)
1503 GCATTC
BstF5I 1768 CATCC GGATG(2/0)
1022 GGATG
DraII 301 GGGCCCC [GA]G^G[ACGT]CC[CT]
1067 GGGACCC
EcoO109I 301 GGGCCCC [GA]G^G[ACGT]CC[CT]
1067 GGGACCC
EcoT22I 1155 ATGCAT ATGCA^T
1501 ATGCAT
FauI 56 CCCGC CCCGC(4/6)
64 GCGGG
FokI 1768 CATCC GGATG(9/13)
1022 GGATG
HphI 384 TCACC GGTGA(8/7)
772 TCACC
MslI 459 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2028 CATGCTCATG
NsiI 1155 ATGCAT ATGCA^T
1501 ATGCAT
SspI 1111 AATATT AAT^ATT
1171 AATATT
XcmI 1530 CCAACACGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1840 CCAAATACTGCCTGG
XmnI 223 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
706 GAACACATTC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BanII BbvI
BcnI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I XhoII