Restriction map for
pFN21AE6334
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BglI BglII BlnI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI Tsp45I TspRI Tth111I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AclI 369 AACGTT AA^CGTT
AflII 729 CTTAAG C^TTAAG
AlwI 747 GGATC GGATC(4/5)
ApaI 394 GGGCCC GGGCC^C
Asp700I 859 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 394 GGGCCC G[GA]GC[CT]^C
BbsI 765 GAAGAC GAAGAC(2/6)
BfrI 729 CTTAAG C^TTAAG
BlpI 12 GCTGAGC GC^T[ACGT]AGC
BmyI 394 GGGCCC G[AGT]GC[ACT]^C
BpmI 644 CTGGAG CTGGAG(16/14)
Bpu1102I 12 GCTGAGC GC^T[ACGT]AGC
BpuAI 765 GAAGAC GAAGAC(2/6)
BsaAI 103 TACGTG [CT]AC^GT[GA]
BsgI 283 CTGCAC GTGCAG(16/14)
BsmI 724 GAATGC GAATGC(1/-1)
Bsp1286I 394 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 480 TGTACA T^GTACA
BsrDI 249 GCAATG GCAATG(2/0)
BsrGI 480 TGTACA T^GTACA
BssHII 30 GCGCGC G^CGCGC
BstEII 73 GGTTACC G^GT[ACGT]ACC
BstPI 73 GGTTACC G^GT[ACGT]ACC
BstYI 747 GGATCT [GA]^GATC[CT]
CelII 12 GCTGAGC GC^T[ACGT]AGC
DraII 393 AGGGCCC [GA]G^G[ACGT]CC[CT]
EarI 580 GAAGAG CTCTTC(1/4)
EcoO109I 393 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 73 GGTTACC G^GT[ACGT]ACC
EcoRI 601 GAATTC G^AATTC
FauI 70 GCGGG CCCGC(4/6)
HapII 59 CCGG C^CGG
HgaI 208 GACGC GACGC(5/10)
HincII 372 GTTGAC GT[CT]^[GA]AC
HindII 372 GTTGAC GT[CT]^[GA]AC
HpaII 59 CCGG C^CGG
Ksp632I 580 GAAGAG CTCTTC(1/4)
MflI 747 GGATCT [GA]^GATC[CT]
MseI 730 TTAA T^TAA
MslI 165 CATCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 59 CCGG C^CGG
PleI 375 GACTC GAGTC(4/5)
Psp1406I 369 AACGTT AA^CGTT
PvuII 272 CAGCTG CAG^CTG
ScaI 460 AGTACT AGT^ACT
SmaI 58 CCCGGG CCC^GGG
SmlI 729 CTTAAG C^T[CT][GA]AG
SspBI 480 TGTACA T^GTACA
StuI 525 AGGCCT AGG^CCT
Tru9I 730 TTAA T^TAA
XhoII 747 GGATCT [GA]^GATC[CT]
XmaCI 58 CCCGGG C^CCGGG
XmaI 58 CCCGGG C^CCGGG
XmnI 859 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 601 GAATTC [GA]^AATT[CT]
708 AAATTT
ApoI 601 GAATTC [GA]^AATT[CT]
708 AAATTT
AvaI 58 CCCGGG C^[CT]CG[GA]G
87 CTCGGG
AvaII 198 GGACC G^G[AT]CC
608 GGTCC
BcnI 58 CCCGG CC^[GC]GG
59 CCGGG
BfaI 464 CTAG C^TAG
803 CTAG
BsmAI 554 GAGAC GTCTC(1/5)
583 GAGAC
BsoBI 58 CCCGGG C^[CT]CG[GA]G
87 CTCGGG
BstF5I 294 CATCC GGATG(2/0)
743 GGATG
FokI 294 CATCC GGATG(9/13)
743 GGATG
MaeI 464 CTAG C^TAG
803 CTAG
MaeII 104 ACGT A^CGT
370 ACGT
MaeIII 74 GTTAC ^GT[ACGT]AC
101 GTTAC
NciI 58 CCCGG CC^[GC]GG
59 CCGGG
PstI 136 CTGCAG CTGCA^G
239 CTGCAG
SfcI 136 CTGCAG C^T[GA][CT]AG
239 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
BbvI BsaJI BsiYI BslI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI HphI
ItaI MboI MboII MnlI MspA1I
MvaI MvnI MwoI NdeII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI TseI Tsp509I TthHB8I