Restriction map for
pFN21AB6990
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstF5I BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NciI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI Tsp45I
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AcyI 24 GGCGCC G[GA]^CG[CT]C
AfaI 742 GTAC GT^AC
AhdI 674 GACAATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 633 GATCC GGATC(4/5)
AspEI 674 GACAATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 487 GAGCAC G[AT]GC[AT]^C
AvaI 228 CTCGGG C^[CT]CG[GA]G
BanI 24 GGCGCC G^G[CT][GA]CC
BanII 5 GAGCCC G[GA]GC[CT]^C
BbeI 24 GGCGCC GGCGC^C
BbvCI 703 GCTGAGG CCTCAGC(-5/-2)
BfaI 212 CTAG C^TAG
BsaHI 24 GGCGCC G[GA]^CG[CT]C
BsaJI 227 CCTCGG C^C[ACGT][ACGT]GG
BsiHKAI 487 GAGCAC G[AT]GC[AT]^C
BsmFI 125 GGGAC GGGAC(10/14)
BsoBI 228 CTCGGG C^[CT]CG[GA]G
Bsp1407I 741 TGTACA T^GTACA
BsrGI 741 TGTACA T^GTACA
BstEII 150 GGTTACC G^GT[ACGT]ACC
BstPI 150 GGTTACC G^GT[ACGT]ACC
Cac8I 21 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 25 GCGC GCG^C
DpnII 633 GATC ^GATC
Eam1105I 674 GACAATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 253 GAAGAG CTCTTC(1/4)
EcoO65I 150 GGTTACC G^GT[ACGT]ACC
FauI 9 CCCGC CCCGC(4/6)
HaeII 24 GGCGCC [GA]GCGC^[CT]
HaeIII 61 GGCC GG^CC
HhaI 25 GCGC GCG^C
Hin1I 24 GGCGCC G[GA]^CG[CT]C
HinP1I 25 GCGC G^CGC
HinfI 298 GATTC G^A[ACGT]TC
HphI 532 GGTGA GGTGA(8/7)
KasI 24 GGCGCC G^GCGCC
Ksp632I 253 GAAGAG CTCTTC(1/4)
MaeI 212 CTAG C^TAG
MaeII 680 ACGT A^CGT
MboI 633 GATC ^GATC
MseI 323 TTAA T^TAA
MslI 506 CATGCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 21 GCCGGC GCC^GGC
NarI 24 GGCGCC GG^CGCC
NdeII 633 GATC ^GATC
NgoMIV 21 GCCGGC G^CCGGC
PflMI 115 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 641 CAGCTG CAG^CTG
RsaI 742 GTAC GT^AC
Sau3AI 633 GATC ^GATC
SfcI 749 CTATAG C^T[GA][CT]AG
SfoI 24 GGCGCC GGC^GCC
SmlI 156 CTCAAG C^T[CT][GA]AG
SspBI 741 TGTACA T^GTACA
SspI 524 AATATT AAT^ATT
TfiI 298 GATTC G^A[AT]TC
Tru9I 323 TTAA T^TAA
TspRI 132 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 115 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
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AciI 10 CCGC CCGC(-3/-1)
19 CCGC
AcsI 445 GAATTT [GA]^AATT[CT]
453 AAATTT
ApoI 445 GAATTT [GA]^AATT[CT]
453 AAATTT
AvaII 81 GGACC G^G[AT]CC
126 GGACC
BbsI 671 GAAGAC GAAGAC(2/6)
171 GTCTTC
BmyI 5 GAGCCC G[AGT]GC[ACT]^C
487 GAGCAC
BpuAI 671 GAAGAC GAAGAC(2/6)
171 GTCTTC
BsmI 472 GAATGC GAATGC(1/-1)
461 GCATTC
Bsp1286I 5 GAGCCC G[AGT]GC[ACT]^C
487 GAGCAC
BsrFI 21 GCCGGC [GA]^CCGG[CT]
27 GCCGGT
BssKI 280 CCTGG ^CC[ACGT]GG
492 CCTGG
BstNI 280 CCTGG CC^[AT]GG
492 CCTGG
Cfr10I 21 GCCGGC [GA]^CCGG[CT]
27 GCCGGT
Cfr13I 81 GGACC G^G[ACGT]CC
126 GGACC
EcoRII 280 CCTGG ^CC[AT]GG
492 CCTGG
HapII 22 CCGG C^CGG
28 CCGG
HpaII 22 CCGG C^CGG
28 CCGG
MaeIII 151 GTTAC ^GT[ACGT]AC
502 GTAAC
MspA1I 10 CCGCTG C[AC]G^C[GT]G
641 CAGCTG
MspI 22 CCGG C^CGG
28 CCGG
MvaI 280 CCTGG CC^[AT]GG
492 CCTGG
NspI 505 ACATGC [GA]CATG^[CT]
744 ACATGC
Sau96I 81 GGACC G^G[ACGT]CC
126 GGACC
ScrFI 280 CCTGG CC^[ACGT]GG
492 CCTGG
SgrAI 20 CGCCGGCG C[GA]^CCGG[CT]G
26 CGCCGGTG
TaqI 293 TCGA T^CGA
723 TCGA
TthHB8I 293 TCGA T^CGA
723 TCGA
XcmI 63 CCATTATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
129 CCACAGTGCTGATGG
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BbvI BsiYI BslI DdeI
Fnu4HI ItaI MboII MnlI MwoI
NlaIII NlaIV TseI Tsp509I