Restriction map for
pFN21AE1462
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BpmI BsaAI BsaBI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TspRI Tth111I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AciI 606 GCGG CCGC(-3/-1)
AcsI 68 AAATTC [GA]^AATT[CT]
AhdI 622 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 145 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 68 AAATTC [GA]^AATT[CT]
Asp700I 583 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 622 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 356 GAGCAC G[AT]GC[AT]^C
AvaI 263 CTCGAG C^[CT]CG[GA]G
BbsI 233 GAAGAC GAAGAC(2/6)
BclI 647 TGATCA T^GATCA
BfaI 565 CTAG C^TAG
BglII 312 AGATCT A^GATCT
BlpI 346 GCTAAGC GC^T[ACGT]AGC
BmyI 356 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 346 GCTAAGC GC^T[ACGT]AGC
BpuAI 233 GAAGAC GAAGAC(2/6)
BsaI 391 GAGACC GGTCTC(1/5)
BsaWI 6 ACCGGA [AT]^CCGG[AT]
BsiHKAI 356 GAGCAC G[AT]GC[AT]^C
BsmFI 227 GGGAC GGGAC(10/14)
BsoBI 263 CTCGAG C^[CT]CG[GA]G
Bsp1286I 356 GAGCAC G[AGT]GC[ACT]^C
BsrDI 593 GCAATG GCAATG(2/0)
BstYI 312 AGATCT [GA]^GATC[CT]
Cac8I 457 GCAGGC GC[ACGT]^[ACGT]GC
CelII 346 GCTAAGC GC^T[ACGT]AGC
EaeI 697 TGGCCG [CT]^GGCC[GA]
Eam1105I 622 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 340 GAAGAG CTCTTC(1/4)
EcoNI 168 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 367 CCTTGG C^C[AT][AT]GG
FauI 606 GCGGG CCCGC(4/6)
FbaI 647 TGATCA T^GATCA
HincII 198 GTCAAC GT[CT]^[GA]AC
HindII 198 GTCAAC GT[CT]^[GA]AC
HindIII 115 AAGCTT A^AGCTT
HinfI 31 GATTC G^A[ACGT]TC
Ksp632I 340 GAAGAG CTCTTC(1/4)
MaeI 565 CTAG C^TAG
MflI 312 AGATCT [GA]^GATC[CT]
MseI 290 TTAA T^TAA
MwoI 423 GCAACAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PaeR7I 263 CTCGAG C^TCGAG
PflMI 362 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmlI 263 CTCGAG C^T[CT][GA]AG
StuI 459 AGGCCT AGG^CCT
StyI 367 CCTTGG C^C[AT][AT]GG
TaqI 264 TCGA T^CGA
TfiI 31 GATTC G^A[AT]TC
Tru9I 290 TTAA T^TAA
Tsp45I 124 GTGAC ^GT[GC]AC
TthHB8I 264 TCGA T^CGA
Van91I 362 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 263 CTCGAG C^TCGAG
XhoII 312 AGATCT [GA]^GATC[CT]
XmnI 583 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbvI 429 GCAGC GCAGC(8/12)
666 GCTGC
BsaJI 13 CCCTGG C^C[ACGT][ACGT]GG
367 CCTTGG
BsmAI 270 GAGAC GTCTC(1/5)
391 GAGAC
BssKI 14 CCTGG ^CC[ACGT]GG
602 CCAGG
BstNI 14 CCTGG CC^[AT]GG
602 CCAGG
BstXI 395 CCAGAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
560 CCAACCTAGTGG
EcoRII 14 CCTGG ^CC[AT]GG
602 CCAGG
Fnu4HI 429 GCAGC GC^[ACGT]GC
666 GCTGC
HaeIII 460 GGCC GG^CC
698 GGCC
HapII 7 CCGG C^CGG
420 CCGG
HpaII 7 CCGG C^CGG
420 CCGG
HphI 100 GGTGA GGTGA(8/7)
654 GGTGA
ItaI 429 GCAGC GC^[ACGT]GC
666 GCTGC
MaeIII 124 GTGAC ^GT[ACGT]AC
259 GTAAC
MboII 233 GAAGA GAAGA(8/7)
340 GAAGA
MspI 7 CCGG C^CGG
420 CCGG
MvaI 14 CCTGG CC^[AT]GG
602 CCAGG
NlaIII 615 CATG CATG^
651 CATG
ScrFI 14 CCTGG CC^[ACGT]GG
602 CCAGG
TseI 429 GCAGC G^C[AT]GC
666 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI AvaII BanI BseRI BsiYI
BslI BstF5I Cfr13I DdeI DpnII
FokI MboI MnlI NdeII NlaIV
Sau3AI Sau96I SfaNI Tsp509I